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Functional interaction of syntaxin and SNAP-25 with voltage-sensitive L- and N-type Ca2+ channels.
Wiser O
,
Bennett MK
,
Atlas D
.
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We have used an electrophysiological assay to investigate the functional interaction of syntaxin 1A and SNAP-25 with the class C, L-type, and the class B, N-type, voltage-sensitive calcium channels. Co-expression of syntaxin 1A with the pore-forming subunits of the L- and N-type channels in Xenopus oocytes generates a dramatic inhibition of inward currents (>60%) and modifies the rate of inactivation (tau) and steady-state voltage dependence of inactivation. Syntaxin 1-267, which lacks the transmembrane region (TMR), and syntaxin 2 do not modify channel properties, suggesting that the syntaxin 1A interaction site resides predominantly in the TMR. Co-expression of SNAP-25 significantly modifies the gating properties of L- and N-type channels and displays modest inhibition of current amplitude. Syntaxin 1A and SNAP-25 combined restore the syntaxin-inhibited N-type inward current but not the reduced rate of inactivation. Hence, a distinct interaction of a putative syntaxin 1A-SNAP-25 complex with the channel is apparent, consistent with the formation of a synaptosomal SNAP receptors (SNAREs) complex. The in vivo functional reconstitution: (i) establishes the proximity of the SNAREs to calcium channels; (ii) provides new insight into a potential regulatory role for the two SNAREs in controlling calcium influx through N- and L-type channels; and (iii) may suggest a pivotal role for calcium channels in the secretion process.
Avidor,
Cardiac L-type Ca2+ channel triggers transmitter release in PC12 cells.
1994,
Pubmed
Baumert,
Synaptobrevin: an integral membrane protein of 18,000 daltons present in small synaptic vesicles of rat brain.
1989,
Pubmed
Bennett,
Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones.
1992,
Pubmed
Bennett,
The syntaxin family of vesicular transport receptors.
1993,
Pubmed
Bennett,
The molecular machinery for secretion is conserved from yeast to neurons.
1993,
Pubmed
Bezprozvanny,
Functional impact of syntaxin on gating of N-type and Q-type calcium channels.
1995,
Pubmed
,
Xenbase
Blasi,
Botulinum neurotoxin C1 blocks neurotransmitter release by means of cleaving HPC-1/syntaxin.
1993,
Pubmed
Calakos,
Vesicle-associated membrane protein and synaptophysin are associated on the synaptic vesicle.
1994,
Pubmed
Chapman,
SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils.
1994,
Pubmed
Dubel,
Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel.
1992,
Pubmed
Ellis,
Sequence and expression of mRNAs encoding the alpha 1 and alpha 2 subunits of a DHP-sensitive calcium channel.
1988,
Pubmed
Gilad,
Identification of the alternative spliced form of the alpha 2/delta subunit of voltage sensitive Ca2+ channels expressed in PC12 cells.
1995,
Pubmed
Hayashi,
Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly.
1994,
Pubmed
Horikawa,
A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals.
1993,
Pubmed
Inoue,
Cloning and sequence analysis of cDNA for a neuronal cell membrane antigen, HPC-1.
1992,
Pubmed
Jay,
Structural characterization of the dihydropyridine-sensitive calcium channel alpha 2-subunit and the associated delta peptides.
1991,
Pubmed
Kushima,
Involvement of HPC-1/syntaxin-1A antigen in transmitter release from PC12h cells.
1995,
Pubmed
Leveque,
The synaptic vesicle protein synaptotagmin associates with calcium channels and is a putative Lambert-Eaton myasthenic syndrome antigen.
1992,
Pubmed
Lévêque,
Purification of the N-type calcium channel associated with syntaxin and synaptotagmin. A complex implicated in synaptic vesicle exocytosis.
1994,
Pubmed
McMahon,
Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein.
1993,
Pubmed
Mochida,
Impairment of syntaxin by botulinum neurotoxin C1 or antibodies inhibits acetylcholine release but not Ca2+ channel activity.
1995,
Pubmed
Niemann,
Clostridial neurotoxins: new tools for dissecting exocytosis.
1994,
Pubmed
Olivera,
Calcium channel diversity and neurotransmitter release: the omega-conotoxins and omega-agatoxins.
1994,
Pubmed
Perez-Reyes,
Cloning and expression of a cardiac/brain beta subunit of the L-type calcium channel.
1992,
Pubmed
,
Xenbase
Pevsner,
Specificity and regulation of a synaptic vesicle docking complex.
1994,
Pubmed
Schiavo,
Botulinum neurotoxin type C cleaves a single Lys-Ala bond within the carboxyl-terminal region of syntaxins.
1995,
Pubmed
Schreibmayer,
Voltage clamping of Xenopus laevis oocytes utilizing agarose-cushion electrodes.
1994,
Pubmed
,
Xenbase
Sheng,
Identification of a syntaxin-binding site on N-type calcium channels.
1994,
Pubmed
Sheng,
Calcium-dependent interaction of N-type calcium channels with the synaptic core complex.
1996,
Pubmed
Söllner,
SNAP receptors implicated in vesicle targeting and fusion.
1993,
Pubmed
Söllner,
A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion.
1993,
Pubmed
Trimble,
VAMP-1: a synaptic vesicle-associated integral membrane protein.
1988,
Pubmed
Wei,
Modification of Ca2+ channel activity by deletions at the carboxyl terminus of the cardiac alpha 1 subunit.
1994,
Pubmed
,
Xenbase
Wei,
Heterologous regulation of the cardiac Ca2+ channel alpha 1 subunit by skeletal muscle beta and gamma subunits. Implications for the structure of cardiac L-type Ca2+ channels.
1991,
Pubmed
,
Xenbase
Wiser,
The alpha 2/delta subunit of voltage sensitive Ca2+ channels is a single transmembrane extracellular protein which is involved in regulated secretion.
1996,
Pubmed
,
Xenbase
Yoshida,
HPC-1 is associated with synaptotagmin and omega-conotoxin receptor.
1992,
Pubmed
