Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Eur Biophys J
2011 Jun 01;406:737-46. doi: 10.1007/s00249-011-0687-2.
Show Gene links
Show Anatomy links
Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients.
Ozu M
,
Dorr RA
,
Teresa Politi M
,
Parisi M
,
Toriano R
.
???displayArticle.abstract???
This work studies water permeability properties of human aquaporin 1 (hAQP1) expressed in Xenopus laevis oocyte membranes, applying a technique where cellular content is replaced with a known medium, with the possibility of measuring intracellular pressure. Consequences on water transport-produced by well-known anisotonic gradients and by the intracellular effect of probable aquaporin inhibitors-were tested. In this way, the specific intracellular inhibition of hAQP1 by the diuretic drug furosemide was demonstrated. In addition, experiments imposing anisotonic mannitol gradients with a constant ionic strength showed that the relationship between water flux and the applied mannitol gradient deflects from a perfect osmometer response when the gradient is higher than 150 mosmol kg (W) (-1) . These results would indicate that the passage of water molecules through hAQP1 may have a maximum rate. As a whole, this work demonstrates the technical advantage of controlling both intracellular pressure and medium composition in order to study biophysical properties of hAQP1, and contributes information on water channel behavior under osmotic challenges and the discovery of new inhibitors.
Abrami,
Sequence and functional expression of an amphibian water channel, FA-CHIP: a new member of the MIP family.
1994, Pubmed,
Xenbase
Abrami,
Sequence and functional expression of an amphibian water channel, FA-CHIP: a new member of the MIP family.
1994,
Pubmed
,
Xenbase
Beckstein,
Liquid-vapor oscillations of water in hydrophobic nanopores.
2003,
Pubmed
Beitz,
Point mutations in the aromatic/arginine region in aquaporin 1 allow passage of urea, glycerol, ammonia, and protons.
2006,
Pubmed
,
Xenbase
Bitner,
Osmotic properties of internally perfused barnacle muscle cells. I. Isosmotic conditions.
2001,
Pubmed
Chandy,
Comparison of the water transporting properties of MIP and AQP1.
1997,
Pubmed
,
Xenbase
Chiu,
Water and polypeptide conformations in the gramicidin channel. A molecular dynamics study.
1989,
Pubmed
Crane,
Long-range nonanomalous diffusion of quantum dot-labeled aquaporin-1 water channels in the cell plasma membrane.
2008,
Pubmed
de Groot,
The dynamics and energetics of water permeation and proton exclusion in aquaporins.
2005,
Pubmed
de Groot,
Water permeation across biological membranes: mechanism and dynamics of aquaporin-1 and GlpF.
2001,
Pubmed
Dorr,
Simple and inexpensive hardware and software method to measure volume changes in Xenopus oocytes expressing aquaporins.
2007,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Hashido,
Water transport in aquaporins: osmotic permeability matrix analysis of molecular dynamics simulations.
2007,
Pubmed
Hill,
Isolation and characterization of the Xenopus oocyte plasma membrane: a new method for studying activity of water and solute transporters.
2005,
Pubmed
,
Xenbase
Hirano,
Molecular mechanisms of how mercury inhibits water permeation through aquaporin-1: understanding by molecular dynamics simulation.
2010,
Pubmed
Jung,
Molecular structure of the water channel through aquaporin CHIP. The hourglass model.
1994,
Pubmed
,
Xenbase
Kelly,
Measurement of elastic properties of Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Kelly,
Direct measurement of intracellular pressure.
1991,
Pubmed
,
Xenbase
Luby-Phelps,
Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area.
2000,
Pubmed
Meinild,
Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5.
1998,
Pubmed
,
Xenbase
Migliati,
Inhibition of aquaporin-1 and aquaporin-4 water permeability by a derivative of the loop diuretic bumetanide acting at an internal pore-occluding binding site.
2009,
Pubmed
,
Xenbase
Ohshima,
Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense: comparison with radish.
2001,
Pubmed
Ozu,
New method to measure water permeability in emptied-out Xenopus oocytes controlling conditions on both sides of the membrane.
2005,
Pubmed
,
Xenbase
Parisi,
From membrane pores to aquaporins: 50 years measuring water fluxes.
2007,
Pubmed
Pickard,
Modelling the swelling assay for aquaporin expression.
2008,
Pubmed
,
Xenbase
Preston,
Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein.
1992,
Pubmed
,
Xenbase
Soveral,
Membrane tension regulates water transport in yeast.
2008,
Pubmed
Taglialatela,
Novel voltage clamp to record small, fast currents from ion channels expressed in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Törnroth-Horsefield,
Structural insights into eukaryotic aquaporin regulation.
2010,
Pubmed
Wan,
Gating of water channels (aquaporins) in cortical cells of young corn roots by mechanical stimuli (pressure pulses): effects of ABA and of HgCl2.
2004,
Pubmed
Yukutake,
Mercury chloride decreases the water permeability of aquaporin-4-reconstituted proteoliposomes.
2008,
Pubmed
,
Xenbase
Zhang,
Expression of mRNA coding for kidney and red cell water channels in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Zhang,
On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes.
2000,
Pubmed
,
Xenbase
