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J Membr Biol
2013 Dec 01;24612:949-58. doi: 10.1007/s00232-013-9598-8.
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Hypotonic regulation of mouse epithelial sodium channel in Xenopus laevis oocytes.
Galizia L
,
Marino GI
,
Ojea A
,
Kotsias BA
.
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The regulation of the epithelial Na⁺ channel (ENaC) during cell swelling is relevant in cellular processes in which cell volume changes occur, i.e., migration, proliferation and cell absorption. Its sensitivity to hypotonically induced swelling was investigated in the Xenopus oocyte expression system with the injection of the three subunits of mouse ENaC. We used voltage-clamp techniques to study the amiloride-sensitive Na⁺ currents (INa(amil)) and video microscopic methodologies to assess oocyte volume changes. Under conditions of mild swelling (25 % reduced hypotonicity) inward current amplitude decreased rapidly over 1.5 min. In contrast, there was no change in current amplitude of H₂O-injected oocytes to the osmotic insult. INa(amil) kinetics analysis revealed a decrease in the slower inactivation time constant during the hypotonic stimuli. Currents from ENaC-injected oocytes were not sensitive to external Cl⁻ reduction. Neither short- nor long-term cytochalasin D treatment affected the observed response. Oocytes expressing a DEG mutant β-ENaC subunit (β-S518K) with an open probability of 1 had reduced INa(amil) hypotonic response compared to oocytes injected with wild-type ENaC subunits. Finally, during the hypotonic response ENaC-injected oocytes did not show a cell volume difference compared with water-injected oocytes. On this basis we suggest that hypotonicity-dependent ENaC inhibition is principally mediated through an effect on open probability of channels in the membrane.
Achard,
Stretch modulates amiloride sensitivity and cation selectivity of sodium channels in human B lymphocytes.
1996, Pubmed
Achard,
Stretch modulates amiloride sensitivity and cation selectivity of sodium channels in human B lymphocytes.
1996,
Pubmed
Amuzescu,
Zinc is a voltage-dependent blocker of native and heterologously expressed epithelial Na+ channels.
2003,
Pubmed
,
Xenbase
Assef,
ENaC channels in oocytes from Xenopus laevis and their regulation by xShroom1 protein.
2011,
Pubmed
,
Xenbase
Awayda,
Regulation of the epithelial Na+ channel by membrane tension.
1998,
Pubmed
,
Xenbase
Awayda,
A cloned renal epithelial Na+ channel protein displays stretch activation in planar lipid bilayers.
1995,
Pubmed
,
Xenbase
Böhmer,
The epithelial Na(+) channel (ENaC) is related to the hypertonicity-induced Na(+) conductance in rat hepatocytes.
2001,
Pubmed
,
Xenbase
Bondarava,
alpha-ENaC is a functional element of the hypertonicity-induced cation channel in HepG2 cells and it mediates proliferation.
2009,
Pubmed
Cantiello,
Actin filaments regulate epithelial Na+ channel activity.
1991,
Pubmed
Carattino,
Epithelial Na+ channels are activated by laminar shear stress.
2004,
Pubmed
,
Xenbase
Chifflet,
A possible role for membrane depolarization in epithelial wound healing.
2005,
Pubmed
Del Mónaco,
Cell migration in BeWo cells and the role of epithelial sodium channels.
2009,
Pubmed
Dorr,
Simple and inexpensive hardware and software method to measure volume changes in Xenopus oocytes expressing aquaporins.
2007,
Pubmed
,
Xenbase
Drummond,
Degenerin/epithelial Na+ channel proteins: components of a vascular mechanosensor.
2004,
Pubmed
Fuller,
Cloning of a bovine renal epithelial Na+ channel subunit.
1995,
Pubmed
,
Xenbase
Galizia,
Functional interaction between AQP2 and TRPV4 in renal cells.
2012,
Pubmed
Grifoni,
ENaC proteins contribute to VSMC migration.
2006,
Pubmed
Grunnet,
Regulation of cloned, Ca2+-activated K+ channels by cell volume changes.
2002,
Pubmed
,
Xenbase
Hermoso,
ClC-3 is a fundamental molecular component of volume-sensitive outwardly rectifying Cl- channels and volume regulation in HeLa cells and Xenopus laevis oocytes.
2002,
Pubmed
,
Xenbase
Hoffmann,
Ion channels involved in cell volume regulation: effects on migration, proliferation, and programmed cell death in non adherent EAT cells and adherent ELA cells.
2011,
Pubmed
Ishikawa,
Electrophysiological characterization of the rat epithelial Na+ channel (rENaC) expressed in MDCK cells. Effects of Na+ and Ca2+.
1998,
Pubmed
Ismailov,
Mechanosensitivity of an epithelial Na+ channel in planar lipid bilayers: release from Ca2+ block.
1997,
Pubmed
Ji,
Osmotic pressure regulates alpha beta gamma-rENaC expressed in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Jiang,
Secondary regulatory volume increase conferred on Xenopus oocytes by expression of AE2 anion exchanger.
1997,
Pubmed
,
Xenbase
Jorgensen,
Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton.
2003,
Pubmed
Kapoor,
Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration.
2009,
Pubmed
Karpushev,
The actin cytoskeleton and small G protein RhoA are not involved in flow-dependent activation of ENaC.
2010,
Pubmed
Karpushev,
Intact cytoskeleton is required for small G protein dependent activation of the epithelial Na+ channel.
2010,
Pubmed
Kelly,
Measurement of elastic properties of Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Kelly,
Control of cell volume in oocytes and eggs from Xenopus laevis.
1995,
Pubmed
,
Xenbase
Laitko,
Membrane tension accelerates rate-limiting voltage-dependent activation and slow inactivation steps in a Shaker channel.
2004,
Pubmed
,
Xenbase
Marino,
The migratory capacity of human trophoblastic BeWo cells: effects of aldosterone and the epithelial sodium channel.
2013,
Pubmed
Mazzochi,
The carboxyl terminus of the alpha-subunit of the amiloride-sensitive epithelial sodium channel binds to F-actin.
2006,
Pubmed
Mills,
Cytoskeletal regulation of membrane transport events.
1994,
Pubmed
Mitchison,
Actin-based cell motility and cell locomotion.
1996,
Pubmed
Morimoto,
Mechanism underlying flow stimulation of sodium absorption in the mammalian collecting duct.
2006,
Pubmed
,
Xenbase
Ozu,
Human AQP1 is a constitutively open channel that closes by a membrane-tension-mediated mechanism.
2013,
Pubmed
,
Xenbase
Palmer,
Gating of Na channels in the rat cortical collecting tubule: effects of voltage and membrane stretch.
1996,
Pubmed
Patel,
Regulation of Kv4.3 voltage-dependent gating kinetics by KChIP2 isoforms.
2004,
Pubmed
,
Xenbase
Plettenberg,
Subunits alpha, beta and gamma of the epithelial Na+ channel (ENaC) are functionally related to the hypertonicity-induced cation channel (HICC) in rat hepatocytes.
2008,
Pubmed
Rooj,
Glioma-specific cation conductance regulates migration and cell cycle progression.
2012,
Pubmed
Schreiber,
Effects of purinergic stimulation, CFTR and osmotic stress on amiloride-sensitive Na+ transport in epithelia and Xenopus oocytes.
2003,
Pubmed
,
Xenbase
Schwab,
Cells move when ions and water flow.
2007,
Pubmed
Schwab,
Functional role of Na+-HCO3- cotransport in migration of transformed renal epithelial cells.
2005,
Pubmed
Segal,
Influence of voltage and extracellular Na(+) on amiloride block and transport kinetics of rat epithelial Na(+) channel expressed in Xenopus oocytes.
2002,
Pubmed
,
Xenbase
Snyder,
Gating induces a conformational change in the outer vestibule of ENaC.
2000,
Pubmed
,
Xenbase
Sparks,
Effects of amiloride on tumor growth and intracellular element content of tumor cells in vivo.
1983,
Pubmed
Ugawa,
Hypotonic stimuli enhance proton-gated currents of acid-sensing ion channel-1b.
2008,
Pubmed
,
Xenbase
Vandorpe,
cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation.
1998,
Pubmed
,
Xenbase
Vila-Carriles,
Surface expression of ASIC2 inhibits the amiloride-sensitive current and migration of glioma cells.
2006,
Pubmed
