Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Abstract
Embryos of Xenopus laevis were selected prior to the onset of innervation and were raised for 2 days in the anaesthetic tricaine methanesulphonate (200 micrograms/ml). The gross development of these tricaine-reared animals appeared normal despite the absence of spontaneous motor activity and the lack of motor responses to prodding with a pin. Motor activity quickly appeared when the anaesthetic was withdrawn. Intracellular recording from the myotomes of intact, tricaine-maintained animals failed to reveal any spontaneous muscle action potentials. Synaptic potentials increased in frequency and amplitude upon withdrawing tricaine, but resting potentials remained unchanged. Cholinesterase activity, detected histochemically, was observed at the ends of the myotomes, the main site of innervation. The intensity of the histochemical reaction product at these sites appeared to be about as great in the myotomes of tricaine-reared animals as in control myotomes. Miniature end-plate currents (m.e.p.c.s), examined by focal external recording, declined with a time constant of 2.9 +/- 0.2 ms (mean +/- S.E. of mean) in the myotomes of tricaine-reared animals (stages 40-41). The time constants in the myotomes of control animals were 1.8 +/- 0.1 ms at stages 40-41 and 8.7 +/- 0.7 ms at stages 24-26 (shortly after the onset of innervation). The anticholinesterase neostigmine doubled m.e.p.c. time constants in the myotomes of tricaine-reared animals as well as in control myotomes at stages 40-41. It is concluded that motor activity is not required for the in vivo development of physiological levels of synaptic cholinesterase in Xenopus myotomal muscle.
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Armstrong,
Muscle activity and the loss of electrical coupling between striated muscle cells in Xenopus embryos.
1983,
Pubmed
,
Xenbase
Betz,
Evolution of cholinergic proteins in developing slow and fast skeletal muscles in chick embryo.
1980,
Pubmed
Blackshaw,
Low resistance junctions between mesoderm cells during development of trunk muscles.
1976,
Pubmed
,
Xenbase
Blackshaw,
Onset of acetylcholine sensitivity and endplate activity in developing myotome muscles of Xenopus.
1976,
Pubmed
,
Xenbase
Chow,
Developmental changes in the distribution of acetylcholine receptors in the myotomes of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Cohen,
The development of neuromuscular connexions in the presence of D-tubocurarine.
1972,
Pubmed
Cohen,
Development of an amphibian neuromuscular junction in vivo and in culture.
1980,
Pubmed
,
Xenbase
DEL CASTILLO,
Localization of active spots within the neuromuscular junction of the frog.
1956,
Pubmed
Eldridge,
Alterations in cat skeletal neuromuscular junctions following prolonged inactivity.
1981,
Pubmed
Frazier,
Tricaine (MS-222): effects on ionic conductances of squid axon membranes.
1975,
Pubmed
Giacobini,
Effects of a snake alpha-neurotoxin on the development of innervated skeletal muscles in chick embryo.
1973,
Pubmed
Gordon,
Possible mechanisms determining synapse formation in developing skeletal muscles of the chick.
1974,
Pubmed
Guth,
Quantitative changes in cholinesterase activity of denervated sole plates following implantation of nerve into muscle.
1966,
Pubmed
Harris,
Embryonic growth and innervation of rat skeletal muscles. II. Neural regulation of muscle cholinesterase.
1981,
Pubmed
Jansen,
Re-innervation of rat skeleton muscle in the presence of alpha-bungarotoxin.
1975,
Pubmed
KARNOVSKY,
THE LOCALIZATION OF CHOLINESTERASE ACTIVITY IN RAT CARDIAC MUSCLE BY ELECTRON MICROSCOPY.
1964,
Pubmed
KATZ,
THE MEASUREMENT OF SYNAPTIC DELAY, AND THE TIME COURSE OF ACETYLCHOLINE RELEASE AT THE NEUROMUSCULAR JUNCTION.
1965,
Pubmed
Katz,
The binding of acetylcholine to receptors and its removal from the synaptic cleft.
1973,
Pubmed
Kullberg,
Development of the myotomal neuromuscular junction in Xenopus laevis: an electrophysiological and fine-structural study.
1977,
Pubmed
,
Xenbase
Kullberg,
Contribution of cholinesterase to developmental decreases in the time course of synaptic potentials at an amphibian neuromuscular junction.
1980,
Pubmed
,
Xenbase
Lømo,
Control of junctional acetylcholinesterase by neural and muscular influences in the rat.
1980,
Pubmed
Moody-Corbett,
Cholinesterase localization at sites of nerve contact on embryonic amphibian muscle cells in culture.
1982,
Pubmed
,
Xenbase
Moody-Corbett,
Localization of cholinesterase at sites of high acetylcholine receptor density on embryonic amphibian muscle cells cultured without nerve.
1981,
Pubmed
,
Xenbase
Obata,
Development of neuromuscular transmission in culture with a variety of neurons and in the presence of cholinergic substances and tetrodotoxin.
1977,
Pubmed
Ohr,
Tricaine methanesulfonate--1. pH and its effects on anesthetic potency.
1976,
Pubmed
Oppenheim,
Embryonic behavior, hatching and neuromuscular development in the chick following a transient reduction of spontaneous motility and sensory input by neuromuscular blocking agents.
1978,
Pubmed
Rieger,
Spontaneous contractile activity and the presence of the 16 S form of acetylcholinesterase in rat muscle cells in culture: reversible suppressive action of tetrodotoxin.
1980,
Pubmed
Rubin,
Regulation of acetylcholinesterase appearance at neuromuscular junctions in vitro.
1980,
Pubmed
Steinbach,
Role of muscle activity in nerve-muscle interaction in vitro.
1974,
Pubmed
Steinbach,
Neuromuscular junctions and alpha-bungarotoxin-binding sites in denervated and contralateral cat skeletal muscles.
1981,
Pubmed
Walicke,
On the role of Ca2+ in the transmitter choice made by cultured sympathetic neurons.
1981,
Pubmed
Weinberg,
Junctional form of acetylcholinesterase restored at nerve-free endplates.
1979,
Pubmed
Weldon,
Development of synaptic ultrastructure at neuromuscular contacts in an amphibian cell culture system.
1979,
Pubmed
,
Xenbase
