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Amiloride-sensitive sodium channel is linked to the cytoskeleton in renal epithelial cells.
Smith PR
,
Saccomani G
,
Joe EH
,
Angelides KJ
,
Benos DJ
.
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Amiloride-sensitive sodium channels are localized to the microvillar domain of apical membranes in sodium-transporting renal epithelial cells. To elucidate the elements that maintain sodium channel distribution at the apical membrane, we searched for specific proteins associating with the channel. Triton X-100 extraction of A6 epithelial cells reveals that sodium channels are associated with detergent-insoluble and assembled cytoskeleton. Indirect immunofluorescence and confocal microscopy show that sodium channels are segregated to the apical microvillar membrane and colocalize with ankyrin, fodrin, and actin. We document by immunoblot analysis that ankyrin and fodrin remain associated with sodium channels after isolation and purification from bovine renal papillae. 125I-labeled ankyrine can be precipitated by anti-sodium-channel antibodies only in the presence of purified bovine sodium-channel complex. Direct binding of 125I-labeled ankyrin shows ankyrin binds to the 150-kDa subunit of the channel. Fluorescence photobleach lateral-diffusion measurements indicate sodium channels are severely restricted in their lateral mobility. We conclude that ankyrin links the amiloride-sensitive sodium channel to the underlying cytoskeleton and this association may sequester sodium channels at apical microvilli and maintain their polarized distribution in renal epithelial cells.
Angelides,
Distribution and lateral mobility of voltage-dependent sodium channels in neurons.
1988, Pubmed
Angelides,
Distribution and lateral mobility of voltage-dependent sodium channels in neurons.
1988,
Pubmed
Bennett,
Proteins involved in membrane--cytoskeleton association in human erythrocytes: spectrin, ankyrin, and band 3.
1983,
Pubmed
Bennett,
Human erythrocyte ankyrin. Purification and properties.
1980,
Pubmed
Bennett,
Spectrin-based membrane skeleton: a multipotential adaptor between plasma membrane and cytoplasm.
1990,
Pubmed
Benos,
The epithelial sodium channel. Subunit number and location of the amiloride binding site.
1987,
Pubmed
Benos,
Purification and characterization of the amiloride-sensitive sodium channel from A6 cultured cells and bovine renal papilla.
1986,
Pubmed
Brown,
Immunocytochemical localization of Na+ channels in rat kidney medulla.
1989,
Pubmed
Coleman,
Functional diversity among spectrin isoforms.
1989,
Pubmed
Davis,
The anion exchanger and Na+K(+)-ATPase interact with distinct sites on ankyrin in in vitro assays.
1990,
Pubmed
Davis,
Brain ankyrin. Purification of a 72,000 Mr spectrin-binding domain.
1984,
Pubmed
Davis,
Diversity in membrane binding sites of ankyrins. Brain ankyrin, erythrocyte ankyrin, and processed erythrocyte ankyrin associate with distinct sites in kidney microsomes.
1989,
Pubmed
Drenckhahn,
Colocalization of band 3 with ankyrin and spectrin at the basal membrane of intercalated cells in the rat kidney.
1985,
Pubmed
Drenckhahn,
Polarized distribution of Mr 210,000 and 190,000 analogs of erythrocyte ankyrin along the plasma membrane of transporting epithelia, neurons and photoreceptors.
1987,
Pubmed
Fey,
Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three-dimensional organization and protein composition.
1984,
Pubmed
Fujimoto,
Immunoelectron microscopy of fodrin in the rat uriniferous and collecting tubular epithelium.
1989,
Pubmed
Gumbiner,
Structure, biochemistry, and assembly of epithelial tight junctions.
1987,
Pubmed
Harris,
Mechanism of cytoskeletal regulation (I): functional differences correlate with antigenic dissimilarity in human brain and erythrocyte spectrin.
1985,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lisanti,
Polarized apical distribution of glycosyl-phosphatidylinositol-anchored proteins in a renal epithelial cell line.
1988,
Pubmed
Lux,
Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins.
1990,
Pubmed
McCloskey,
Protein diffusion in cell membranes: some biological implications.
1984,
Pubmed
Morrow,
Ankyrin links fodrin to the alpha subunit of Na,K-ATPase in Madin-Darby canine kidney cells and in intact renal tubule cells.
1989,
Pubmed
Nelson,
Dynamics of membrane-skeleton (fodrin) organization during development of polarity in Madin-Darby canine kidney epithelial cells.
1986,
Pubmed
Ojakian,
The polarized distribution of an apical cell surface glycoprotein is maintained by interactions with the cytoskeleton of Madin-Darby canine kidney cells.
1988,
Pubmed
Rodman,
Cytoskeletal proteins of the rat kidney proximal tubule brush border.
1986,
Pubmed
Rodriguez-Boulan,
Morphogenesis of the polarized epithelial cell phenotype.
1989,
Pubmed
Salas,
Selective anchoring in the specific plasma membrane domain: a role in epithelial cell polarity.
1988,
Pubmed
Sariban-Sohraby,
Detergent solubilization, functional reconstitution, and partial purification of epithelial amiloride-binding protein.
1986,
Pubmed
Sorscher,
Antibodies against purified epithelial sodium channel protein from bovine renal papilla.
1988,
Pubmed
Srinivasan,
Ankyrin and spectrin associate with voltage-dependent sodium channels in brain.
1988,
Pubmed
Tousson,
Immunochemical localization of amiloride-sensitive sodium channels in sodium-transporting epithelia.
1989,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
