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EMBO J
1990 Jun 01;96:1859-66. doi: 10.1002/j.1460-2075.1990.tb08311.x.
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Selective photochemical treatment of oestrogen receptor in a Xenopus liver extract destroys hormone binding and transcriptional activation but not DNA binding.
Cridland NA
,
Wright CV
,
McKenzie EA
,
Knowland J
.
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Photochemical excitation of a simple derivative of oestradiol using light in the UV-A range totally, permanently and selectively inactivates the oestrogen receptor protein present in a Xenopus liver extract without affecting its overall size. Inactivation of the binding site proceeds to completion with simple, first-order kinetics. Inactivation is prevented by excess oestradiol but not by non-oestrogenic steroids. Using an in vitro transcription system, we show that the treatment eliminates transcription of vitellogenin genes, which are normally oestrogen-responsive, but has no effect on the transcription of albumin genes, which are not. Native receptor binds to the two imperfectly palindromic sequences in the vitellogenin B2 gene which together constitute an oestrogen-response unit. Its affinity for one sequence is greater than its affinity for the other, suggesting that a compulsory binding order operates when receptor interacts with the B2 gene. Photoinactivated receptor still binds to both sequences, but with reduced affinity. We also discuss our findings in the context of the current concern over the effects of UV-A on human tissues.
Everett,
Penetration of epidermis by ultraviolet rays.
1966, Pubmed
Everett,
Penetration of epidermis by ultraviolet rays.
1966,
Pubmed
Germond,
Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.
1983,
Pubmed
,
Xenbase
Katzenellenbogen,
Chemical probes of estrogen action: affinity labeling.
1976,
Pubmed
Katzenellenbogen,
Photoreactivity of some light-sensitive estrogen derivatives. Use of an exchange assay to determine their photointeraction with the rat uterine estrogen binding protein.
1974,
Pubmed
Kazmaier,
Post-transcriptional regulation of albumin gene expression in Xenopus liver.
1985,
Pubmed
,
Xenbase
Klein-Hitpass,
Specific binding of estrogen receptor to the estrogen response element.
1989,
Pubmed
,
Xenbase
Klein-Hitpass,
Synergism of closely adjacent estrogen-responsive elements increases their regulatory potential.
1988,
Pubmed
,
Xenbase
Knuppen,
Synthesis of [2H8] estradiol, [2H7] estrone, [2H6] 2-hydroxyestrone and [2H6] 4-hydroxyestrone as internal standards for selected ion monitoring.
1982,
Pubmed
Kumar,
Localisation of the oestradiol-binding and putative DNA-binding domains of the human oestrogen receptor.
1986,
Pubmed
Martinez,
Cooperative binding of estrogen receptor to imperfect estrogen-responsive DNA elements correlates with their synergistic hormone-dependent enhancer activity.
1989,
Pubmed
,
Xenbase
Martyr,
Chemical modification of amino acid residues associated with the delta-4-3-ketosteroid-dependent photoinactivation of delta-5-3-ketosteroid isomerase.
1975,
Pubmed
May,
Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.
1982,
Pubmed
,
Xenbase
Metzger,
The human oestrogen receptor functions in yeast.
1988,
Pubmed
Ogez,
A novel chemical modification of delta 5-3-ketosteroid isomerase occurring during its 3-oxo-4-estren-17 beta-yl acetate-dependent photoinactivation.
1977,
Pubmed
Roza,
UVA hazards in skin associated with the use of tanning equipment.
1989,
Pubmed
ten Heggeler-Bordier,
Electron microscopic visualization of protein-DNA interactions at the estrogen responsive element and in the first intron of the Xenopus laevis vitellogenin gene.
1987,
Pubmed
,
Xenbase
Tora,
The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties.
1989,
Pubmed
Tsai,
Cooperative binding of steroid hormone receptors contributes to transcriptional synergism at target enhancer elements.
1989,
Pubmed
Tyrrell,
Singlet oxygen involvement in the inactivation of cultured human fibroblasts by UVA (334 nm, 365 nm) and near-visible (405 nm) radiations.
1989,
Pubmed
Wahli,
Evolution and expression of vitellogenin genes.
1988,
Pubmed
Walker,
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
1984,
Pubmed
,
Xenbase
Wangh,
Synthesis of vitellogenin in cultures of male and female frog liver regulated by estradiol treatment in vitro.
1975,
Pubmed
,
Xenbase
Waterman,
A single domain of the estrogen receptor confers deoxyribonucleic acid binding and transcriptional activation of the rat prolactin gene.
1988,
Pubmed
Webster,
The transcriptional activation function located in the hormone-binding domain of the human oestrogen receptor is not encoded in a single exon.
1989,
Pubmed
Westley,
An estrogen receptor from Xenopus laevis liver possibly connected with vitellogenin synthesis.
1978,
Pubmed
,
Xenbase
Westley,
Effects of antiestrogens on the estrogen-regulated pS2 RNA and the 52- and 160-kilodalton proteins in MCF7 cells and two tamoxifen-resistant sublines.
1984,
Pubmed
Wright,
Partial purification of estradiol receptor from Xenopus laevis liver and levels of receptor in relation to estradiol concentration.
1983,
Pubmed
,
Xenbase
