Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The 10 kb Drosophila virilis 28S rDNA intervening sequence is flanked by a direct repeat of 14 base pairs of coding sequence.
Rae PM
,
Kohorn BD
,
Wade RP
.
Abstract
Most repeat units of rDNA in Drosophila virilis are interrupted in the 28S rRNA coding region by an intervening sequence about 10 kb in length; uninterrupted repeats have a length of about 11 kb. We have sequenced the coding/intervening sequence junctions and flanking regions in two independent clones of interrupted rDNA, and the corresponding 28S rRNA coding region in a clone of uninterrupted rDNA. The intervening sequence is terminated at both ends by a direct repeat of a fourteen nucleotide sequence that is present once in the corresponding region of an intact gene. This is a phenomenon associated with transposable elements in other eukaryotes and in prokaryotes, and the Drosophila rDNA intervening sequence is discussed in this context. We have compared more than 200 nucleotides of the D. virilis 28S rRNA gene with sequences of homologous regions of rDNA in Tetrahymena pigmentosa (Wild and Sommer, 1980) and Xenopus laevis (Gourse and Gerbi, 1980): There is 93% sequence homology among the diverse species, so that the rDNA region in question (about two-thirds of the way into the 28S rRNA coding sequence) has been very highly conserved in eukaryote evolution. The intervening sequence in T. pigmentosa is at a site 79 nucleotides upstream from the insertion site of the Drosophila intervening sequence.
Barnett,
A 9.6 kb intervening sequence in D. virilis rDNA, and sequence homology in rDNA interruptions of diverse species of Drosophila and other diptera.
1979, Pubmed
Barnett,
A 9.6 kb intervening sequence in D. virilis rDNA, and sequence homology in rDNA interruptions of diverse species of Drosophila and other diptera.
1979,
Pubmed
Beckingham,
The ribosomal DNA of Calliphora erythrocephala; and analysis of hybrid plasmids containing ribosomal DNA.
1980,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Cameron,
Evidence for transposition of dispersed repetitive DNA families in yeast.
1979,
Pubmed
Campbell,
Unique sequence arrangement of ribosomal genes in the palindromic rDNA molecule of Physarum polycephalum.
1979,
Pubmed
Chooi,
The occurrence of long transcription units among the X and Y ribosomal genes of Drosophila melanogaster: transcription of insertion sequences.
1979,
Pubmed
Dawid,
Sequences homologous to ribosomal insertions occur in the Drosophila genome outside the nucleolus organizer.
1977,
Pubmed
Dawid,
Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments.
1978,
Pubmed
Din,
The intervening sequence in the 26S rRNA coding region of T. thermophila is transcribed within the largest stable precursor for rRNA.
1979,
Pubmed
Din,
Extrachromosomal ribosomal RNA genes in Tetrahymena: structure and evolution.
1979,
Pubmed
Finnegan,
Repeated gene families in Drosophila melanogaster.
1978,
Pubmed
Gubler,
The gene for the 26 S rRNA in Physarum contains two insertions.
1979,
Pubmed
Jolly,
Nuclear RNA transcripts from Drosophila melanogaster ribosomal RNA genes containing introns.
1980,
Pubmed
Kleckner,
Translocatable elements in procaryotes.
1977,
Pubmed
Long,
Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.
1979,
Pubmed
Long,
Expression of ribosomal DNA insertions in Drosophila melanogaster.
1979,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Pellegrini,
Sequence arrangement of the rDNA of Drosophila melanogaster.
1977,
Pubmed
Potter,
Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila.
1979,
Pubmed
Smith,
A simple method for DNA restriction site mapping.
1976,
Pubmed
Smith,
Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
1979,
Pubmed
Strobel,
Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila.
1979,
Pubmed
Wellauer,
The structural organization of ribosomal DNA in Drosophila melanogaster.
1977,
Pubmed
Wellauer,
Ribosomal DNA in Drosophila melanogaster. II. Heteroduplex mapping of cloned and uncloned rDNA.
1978,
Pubmed
White,
R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.
1977,
Pubmed
Wild,
An intervening sequence in the gene coding for 25S ribosomal RNA of Tetrahymena pigmentosa.
1979,
Pubmed
Wild,
Sequence of a ribosomal RNA gene intron from Tetrahymena.
1980,
Pubmed
Zaug,
In vitro splicing of the ribosomal RNA precursor in nuclei of Tetrahymena.
1980,
Pubmed
