XB-ART-7386
Science
2002 Mar 29;2955564:2452-6. doi: 10.1126/science.1068798.
Show Gene links
Show Anatomy links
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
???displayArticle.abstract???
The small guanosine triphosphatase Ran is loaded with guanosine triphosphate (GTP) by the chromatin-bound guanine nucleotide exchange factor RCC1 and releases import cargoes in the nucleus during interphase. In mitosis, Ran-GTP promotes spindle assembly around chromosomes by locally discharging cargoes that regulate microtubule dynamics and organization. We used fluorescence resonance energy transfer-based biosensors to visualize gradients of Ran-GTP and liberated cargoes around chromosomes in mitotic Xenopus egg extracts. Both gradients were required to assemble and maintain spindle structure. During interphase, Ran-GTP was highly enriched in the nucleoplasm, and a steep concentration difference between nuclear and cytoplasmic Ran-GTP was established, providing evidence for a Ran-GTP gradient surrounding chromosomes throughout the cell cycle.
???displayArticle.pubmedLink??? 11923538
???displayArticle.link??? Science
Species referenced: Xenopus
Genes referenced: ran rcc1