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Effects of estradiol-17beta in the male Xenopus laevis: isolation and translation of cytoplasmic messenger RNA populations.
Wiggins TL
,
Tucciarone LM
,
Lanclos KD
.
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Total Xenopus liver cytoplasmic RNA isolated following long-term estrogen administration (14 days) was fractioned using Sepharose 4B chromatography. One of the Sepharose 4B peaks was shown to contain RNA with a molecular weight reported for vitellogenin mRNA (approximately 34S). The presence of estrogen-induced vitellogenin mRNA in the peak 5 RNA was determined by translation of the RNA in the oocyte and analysis of the oocyte translational products by immunoprecipitation with anti-vitellogenin. Sepharose 4B peaks 2 and 3 were also observed to contain estrogen induced mRNA populations sedimenting between 9--18S. These findings suggest that Sepharose 4B chromatography might prove useful in separating different mRNA populations following estrogen-induced gene activation.
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976, Pubmed,
Xenbase
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976,
Pubmed
,
Xenbase
Clemens,
Sequential changes in the protein synthetic activity of male Xenopus laevis liver following induction of egg-yolk proteins by Estradiol-17 beta.
1975,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Lanclos,
Translation of hormone-induced messenger RNA in amphibian oocytes: I. Induction by estrogen of messenger RNA encoded for vitellogenic protein in the liver of the male African clawed toad (Xenopus laevis).
1975,
Pubmed
,
Xenbase
O'Malley,
Female steroid hormones and target cell nuclei.
1974,
Pubmed
Palmiter,
Modulation of ovalbumin synthesis by estradiol-17 beta and actinomycin D as studied in explants of chick oviduct in culture.
1971,
Pubmed
Shapiro,
In vitro translation and estradiol-17beta induction of Xenopus laevis vitellogenin messenger RNA.
1976,
Pubmed
,
Xenbase
STEELE,
EFFECTS OF THIOACETAMIDE ON THE COMPOSITION AND BIOSYNTHESIS OF NUCLEOLAR AND NUCLEAR RIBONUCLEIC ACID IN RAT LIVER.
1965,
Pubmed
Tata,
The expression of the vitellogenin gene.
1976,
Pubmed
,
Xenbase
Wallace,
The induced synthesis and transport of yolk proteins and their accumulation by the oocyte in Xenopus laevis.
1968,
Pubmed
,
Xenbase
Woo,
Use of Sepharose 4B for preparative scale fractionation of eukaryotic messenger RNA's.
1974,
Pubmed
