Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Wilehm Roux Arch Dev Biol
1981 Mar 01;1902:97-102. doi: 10.1007/BF00848402.
Show Gene links
Show Anatomy links
Cloning of cDNA sequences derived from poly(A)+ nuclear RNA ofXenopus laevis at different developmental stages: Evidence for stage specific regulation.
Knöchel W
,
Bladauski D
.
???displayArticle.abstract???
Nuclear poly(A)+ RNA was isolated from gastrula and early tadpole stages ofXenopus laevis, transcribed into cDNA and integrated as double stranded cDNA by the G-C joining method into the Pstcleavage site of plasmid pBR 322. After cloning inE. coli strain HB 101 the clone libraries were hybridized to32P labelled cDNA derived from nuclear poly(A)+ RNA of the two different developmental stages. About 20% of the clones gave a positive hybridization signal thus representing RNA molecules of high and medium abundance. From these clones, some individual clones were identified containing sequences which are not present at the oocyte and gastrula stages but which are transcribed at the early tadpole stage of embryonic development.
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972, Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Cohen,
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
1972,
Pubmed
Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed
Dworkin,
Construction of a cloned library of expressed embryonic gene sequences from Xenopus laevis.
1980,
Pubmed
,
Xenbase
Dworkin,
Use of a cloned library for the study of abundant poly(A)+RNA during Xenopus laevis development.
1980,
Pubmed
,
Xenbase
Galau,
Structural gene sets active in embryos and adult tissues of the sea urchin.
1976,
Pubmed
Gergen,
Filter replicas and permanent collections of recombinant DNA plasmids.
1979,
Pubmed
Grundmann,
Sequences coding for proteins expressed in liver and for globin in poly(A)+ and poly(A)- RNA fractions from nuclei and cytoplasm of chicken immature red blood cells.
1978,
Pubmed
Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed
Holtfreter,
[Not Available].
1931,
Pubmed
Jacob,
Characterization of cloned cDNA sequences derived from Xenopus laevis poly A(+) oocyte RNA.
1980,
Pubmed
,
Xenbase
Kleene,
Similarity of hnRNA sequences in blastula and pluteus stage sea urchin embryos.
1977,
Pubmed
Knöchel,
Cleavage sites of restriction endonucleases EcoRI, BamHI and HindIII on chicken embryo rDNA.
1979,
Pubmed
Knöchel,
A comparison of sequence complexity of nuclear and polysomal poly(A)+ RNA from different developmental stages ofXenopus laevis.
1980,
Pubmed
,
Xenbase
Perlman,
Analysis of Xenopus laevis ovary and somatic cell polyadenylated RNA by molecular hybridization.
1978,
Pubmed
,
Xenbase
Philippsen,
Cloning of the yeast ribosomal DNA repeat unit in SstI and HindIII lambda vectors using genetic and physical size selections.
1978,
Pubmed
Rougeon,
Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
1975,
Pubmed
Rowekamp,
Isolation of developmentally regulated genes from Dictyostelium.
1980,
Pubmed
Roychoudhury,
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
1976,
Pubmed
Sagata,
A study on the steady-state population of poly(A)+RNA during early development of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Shepherd,
Post-transcriptional control of messenger RNA diversity in frog embryos.
1979,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Van Ness,
Sequence complexity of cDNA transcribed from a diverse mRNA population.
1980,
Pubmed
Warner,
Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA.
1966,
Pubmed
Wensink,
A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
1974,
Pubmed
Williams,
Changes in the abundance of polyadenylated RNA during slime mould development measured using cloned molecular hybridization probes.
1979,
Pubmed
Wold,
Sea urchin embryo mRNA sequences expressed in the nuclear RNA of adult tissues.
1978,
Pubmed