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A cell-free cytoplasmic preparation from activated Rana pipiens eggs could induce in demembranated Xenopus laevis sperm nuclei morphological changes similar to those seen during pronuclear formation in intact eggs. The condensed sperm chromatin underwent an initial rapid, but limited, dispersion. A nuclear envelope formed around the dispersed chromatin and the nuclei enlarged. The subcellular distribution of the components required for these changes was examined by separating the preparations into soluble (cytosol) and particulate fractions by centrifugation at 150,000 g for 2 h. Sperm chromatin was incubated with the cytosol or with the particulate material after it had been resuspended in either the cytosol, heat-treated (60 or 100 degrees C) cytosol or buffer. We found that the limited dispersion of chromatin occurred in each of these ooplasmic fractions, but not in the buffer alone. Nuclear envelope assembly required the presence of both untreated cytosol and particulate material. Ultrastructural examination of the sperm chromatin during incubation in the preparations showed that membrane vesicles of approximately 200 nm in diameter, found in the particulate fraction, flattened and fused together to contribute the membranous components of the nuclear envelope. The enlargement of the sperm nuclei occurred only after the nuclear envelope formed. The pronuclei formed in the cell-free preparations were able to incorporate [3H]dTTP into DNA. This incorporation was inhibited by aphidicolin, suggesting that the DNA synthesis by the pronuclei was dependent on DNA polymerase-alpha. When sperm chromatin was incubated greater than 3 h, the chromatin of the pronuclei often recondensed to form structures resembling mitotic chromosomes within the nuclear envelope. Therefore, it appeared that these ooplasmic preparations could induce, in vitro, nuclear changes resembling those seen during the first cell cycle in the zygote.
Anderson,
Changing rates of DNA and RNA synthesis in Drosophila embryos.
1981, Pubmed
Anderson,
Changing rates of DNA and RNA synthesis in Drosophila embryos.
1981,
Pubmed
Arms,
Cytonucleoproteins in cleaving eggs of Xenopus laevis.
1968,
Pubmed
,
Xenbase
Barry,
Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs.
1972,
Pubmed
,
Xenbase
Benbow,
Cytoplasmic control of nuclear DNA synthesis during early development of Xenopus laevis: a cell-free assay.
1975,
Pubmed
,
Xenbase
Carroll,
Changes in the histones of the sea urchin Stronglylocentrotus purpuratus at fertilization.
1979,
Pubmed
Chai,
Ultrastructural changes in the nuclear envelope during mitosis of Chinese hamster cells: a proposed mechanism of nuclear envelope reformation.
1974,
Pubmed
Das,
Cytochemical studies on the protamine-type protein transition in sperm nuclei after fertilization and the early embryonic histones of Urechis caupo.
1975,
Pubmed
Ecklund,
Mouse sperm basic nuclear protein. Electrophoretic characterization and fate after fertilization.
1975,
Pubmed
Franke,
Structure, biochemistry, and functions of the nuclear envelope.
1974,
Pubmed
Gerace,
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
1978,
Pubmed
Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed
Graham,
The regulation of DNA synthesis and mitosis in multinucleate frog eggs.
1966,
Pubmed
Gulyas,
The rabbit zygote. 3. Formation of the blastomere nucleus.
1972,
Pubmed
Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase
HINEGARDNER,
THE DNA SYNTHETIC PERIOD DURING EARLY DEVELOPMENT OF THE SEA URCHIN EGG.
1964,
Pubmed
Hoffner,
The acquisition of egg cytoplasmic non-histone proteins by nuclei during nuclear reprogramming.
1977,
Pubmed
Huchon,
Germinal vesicle breakdown in the Xenopus laevis oocyte: description of a transient microtubular structure.
1981,
Pubmed
,
Xenbase
Ikegami,
Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha.
1978,
Pubmed
Johnson,
Nucleo-cytoplasmic interactions in the acheivement of nuclear synchrony in DNA synthesis and mitosis in multinucleate cells.
1971,
Pubmed
Jost,
Nuclear lamina assembly, synthesis and disaggregation during the cell cycle in synchronized HeLa cells.
1981,
Pubmed
Katagiri,
Spermatozoan response to the toad egg matured after removal of germinal vesicle.
1976,
Pubmed
Kunkle,
Nuclear protein changes in the maternally and paternally derived chromatin at fertilization.
1978,
Pubmed
Leonard,
Induction of DNA synthesis in amphibian erythroid nuclei in Rana eggs following conditioning in meiotic oocytes.
1982,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lohka,
The germinal vesicle material required for sperm pronuclear formation is located in the soluble fraction of egg cytoplasm.
1983,
Pubmed
,
Xenbase
Longo,
Fertilization: a comparative ultrastructural review.
1973,
Pubmed
Longo,
Derivation of the membrane comprising the male pronuclear envelope in inseminated sea urchin eggs.
1976,
Pubmed
Longo,
Transformations of sperm nuclei upon insemination.
1978,
Pubmed
Longo,
The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata.
1968,
Pubmed
Longo,
An ultrastructural analysis of mitosis and cytokinesis in the zygote of the sea urchin, Arbacia punctulata.
1972,
Pubmed
Masui,
Oscillatory activity of maturation promoting factor (MPF) in extracts of Rana pipiens eggs.
1982,
Pubmed
Masui,
Relative roles of the pituitary, follicle cells, and progesterone in the induction of oocyte maturation in Rana pipiens.
1967,
Pubmed
Maul,
Nuclear pore complexes. Elimination and reconstruction during mitosis.
1977,
Pubmed
Maul,
The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.
1977,
Pubmed
Merriam,
Movement of cytoplasmic proteins into nuclei induced to enlarge and initiate DNA or RNA synthesis.
1969,
Pubmed
Meyerhof,
Ca and Mg control of cytostatic factors from Rana pipiens oocytes which cause metaphase and cleavage arrest.
1977,
Pubmed
Poccia,
Transitions in histone variants of the male pronucleus following fertilization and evidence for a maternal store of cleavage-stage histones in the sera urchin egg.
1981,
Pubmed
Rodman,
Turnover of basic chromosomal proteins in fertilized eggs: a cytoimmunochemical study of events in vivo.
1981,
Pubmed
Skoblina,
Role of karyoplasm in the emergence of capacity of egg cytoplasm to induce DNA synthesis in transplanted sperm nuclei.
1976,
Pubmed
Szollosi,
The nuclear envelope: its breakdown and fate in mammalian oogonia and oocytes.
1972,
Pubmed
Yanagimachi,
Electron microscope studies of sperm incorporation into the golden hamster egg.
1970,
Pubmed
Ziegler,
Control of chromosome behavior in amphibian oocytes. I. The activity of maturing oocytes inducing chromosome condensation in transplanted brain nuclei.
1973,
Pubmed