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XB-ART-4588
Neuron 2003 Sep 25;401:15-23. doi: 10.1016/s0896-6273(03)00570-1.
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Charybdotoxin binding in the I(Ks) pore demonstrates two MinK subunits in each channel complex.

Chen H , Kim LA , Rajan S , Xu S , Goldstein SA .


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I(Ks) voltage-gated K(+) channels contain four pore-forming KCNQ1 subunits and MinK accessory subunits in a number that has been controversial. Here, I(Ks) channels assembled naturally by monomer subunits are compared to those with linked subunits that force defined stoichiometries. Two strategies that exploit charybdotoxin (CTX)-sensitive subunit variants are applied. First, CTX on rate, off rate, and equilibrium affinity are found to be the same for channels of monomers and those with a fixed 2:4 MinK:KCNQ1 valence. Second, 3H-CTX and an antibody are used to directly quantify channels and MinK subunits, respectively, showing 1.97 +/- 0.07 MinK per I(Ks) channel. Additional MinK subunits do not enter channels of monomeric subunits or those with fixed 2:4 valence. We conclude that two MinK subunits are necessary, sufficient, and the norm in I(Ks) channels. This stoichiometry is expected for other K(+) channels that contain MinK or MinK-related peptides (MiRPs).

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Species referenced: Xenopus
Genes referenced: kcne1 kcnq1 mink1 vsig1