Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1984 Nov 01;311:2445-52. doi: 10.1002/j.1460-2075.1984.tb02154.x.
Show Gene links
Show Anatomy links
Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells.
Laski FA
,
Belagaje R
,
Hudziak RM
,
Capecchi MR
,
Norton GP
,
Palese P
,
RajBhandary UL
,
Sharp PA
.
???displayArticle.abstract???
We have used site-specific mutagenesis to change the anticodon of a Xenopus laevis tyrosine tRNA gene so that it would recognize ochre codons. This tRNA gene is expressed when amplified in monkey cells as part of a SV40 recombinant and efficiently suppresses termination at both the ochre codon separating the adenovirus 2 hexon gene from a 23-kd downstream gene and the ochre codon at the end of the NS1 gene of influenza virus A/Tex/1/68. Termination at an amber codon of a NS1 gene of another influenza virus strain was not suppressed by the (Su+) ochre gene suggesting that in mammalian cells amber codons are not recognized by ochre suppressor tRNAs. Finally, microinjection into mammalian cells of both (Su+) ochre tRNA genes and selectible genes containing ochre nonsense mutations gives rise to colonies under selective conditions. We conclude that it should be possible to isolate a wide assortment of mammalian cell lines with ochre suppressor activity.
Akusjärvi,
The sequence of the 3' non-coding region of the hexon mRNA discloses a novel adenovirus gene.
1981, Pubmed
Akusjärvi,
The sequence of the 3' non-coding region of the hexon mRNA discloses a novel adenovirus gene.
1981,
Pubmed
Baez,
Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains.
1980,
Pubmed
Bossi,
The influence of codon context on genetic code translation.
1980,
Pubmed
Bossi,
Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message.
1983,
Pubmed
Capecchi,
The isolation of a suppressible nonsense mutant in mammalian cells.
1977,
Pubmed
Cepko,
Immunoprecipitation with two-dimensional pools as a hybridoma screening technique: production and characterization of monoclonal antibodies against adenovirus 2 proteins.
1981,
Pubmed
Chang,
beta 0 thalassemia, a nonsense mutation in man.
1979,
Pubmed
Cremer,
In vitro suppression of UAG and UGA mutants in the thymidine kinase gene of herpes simplex virus.
1979,
Pubmed
Crick,
Codon--anticodon pairing: the wobble hypothesis.
1966,
Pubmed
Fiers,
Complete nucleotide sequence of SV40 DNA.
1978,
Pubmed
Gesteland,
Identification of amber and ochre mutants of the human virus Ad2+ND1.
1977,
Pubmed
Gilmore,
Amino acid replacements resulting from super-suppression of nonsense mutants of iso-1-cytochrome c from yeast.
1971,
Pubmed
Grodzicker,
Conditional lethal mutants of adenovirus type 2-simian virus 40 hybrids. II. Ad2+ND1 host-range mutants that synthesize fragments of the Ad2+ND1 30K protein.
1976,
Pubmed
Horowitz,
Expression of chimeric genes in the early region of SV40.
1983,
Pubmed
Hudziak,
Establishment of mammalian cell lines containing multiple nonsense mutations and functional suppressor tRNA genes.
1982,
Pubmed
,
Xenbase
Kohli,
Usage of the three termination codons: compilation and analysis of the known eukaryotic and prokaryotic translation termination sequences.
1981,
Pubmed
,
Xenbase
Krystal,
Sequential mutations in the NS genes of influenza virus field strains.
1983,
Pubmed
Kudo,
Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.
1981,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Laski,
An amber suppressor tRNA gene derived by site-specific mutagenesis: cloning and function in mammalian cells.
1982,
Pubmed
,
Xenbase
Laski,
Expression of a X. laevis tRNATyr gene in mammalian cells.
1982,
Pubmed
,
Xenbase
Laski,
Characterization of tRNA precursor splicing in mammalian extracts.
1983,
Pubmed
,
Xenbase
Miller,
Effects of surrounding sequence on the suppression of nonsense codons.
1983,
Pubmed
Müller,
Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.
1980,
Pubmed
,
Xenbase
Narang,
Improved phosphotriester method for the synthesis of gene fragments.
1979,
Pubmed
Parvin,
Nonsense mutations affecting the lengths of the NS1 nonstructural proteins of influenza A virus isolates.
1983,
Pubmed
Rawlins,
Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis.
1980,
Pubmed
Summers,
Functional suppression in mammalian cells of nonsense mutations in the herpes simplex virus thymidine kinase gene by suppressor tRNA genes.
1983,
Pubmed
Weber,
Genetic analysis of adenovirus type 2 III. Temperature sensitivity of processing viral proteins.
1976,
Pubmed
Young,
Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli.
1983,
Pubmed
Young,
Measurement of suppressor transfer RNA activity.
1983,
Pubmed
