Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
In vivo aminoacylation of human and Xenopus suppressor tRNAs constructed by site-specific mutagenesis.
Ho YS
,
Kan YW
.
Abstract
Amber suppressor tRNA genes were constructed by site-specific mutagenesis of the anticodons of human lysine-inserting tRNA (tRNA(Lys)) and glutamine-inserting tRNA (tRNA(Gln)) genes, and a Xenopus laevis tyrosine-inserting tRNA (tRNA(Tyr)) gene. As previous in vitro studies in prokaryotes have shown that substitution of nucleotides in the anticodon region can profoundly affect tRNA aminoacylation, it is important to determine whether the mutation affects aminoacylation of these eukaryotic tRNAs. We present a method for quantitating the tRNA aminoacylation in vivo in mammalian cells, and we have determined that the suppressor tRNA(Tyr) is fully aminoacylated and suppressor tRNA(Lys) and tRNA(Gln) are aminoacylated 40-50% and 80%, respectively. This in vivo method of estimating aminoacylation may be applied to other mutations in the tRNA genes.
Alwine,
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
1979, Pubmed
Alwine,
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
1979,
Pubmed
Bare,
Aminoacylation of anticodon loop substituted yeast tyrosine transfer RNA.
1985,
Pubmed
Bruce,
Enzymatic replacement of the anticodon of yeast phenylalanine transfer ribonucleic acid.
1982,
Pubmed
Bruce,
Specific interaction of anticodon loop residues with yeast phenylalanyl-tRNA synthetase.
1982,
Pubmed
Capone,
Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene.
1985,
Pubmed
Carbon,
Genetically altered tRNA-Gly subspecies in E. coli.
1969,
Pubmed
Carbon,
Genetically and chemically derived missense suppressor transfer RNA's with altered enzymic aminoacylation rates.
1968,
Pubmed
Carbon,
Glycine transfer RNA of Escherichia coli. II. Impaired GGA-recognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation.
1970,
Pubmed
Celis,
Suppressor su+7 inserts tryptophan in addition to glutamine.
1976,
Pubmed
Chang,
beta 0 thalassemia, a nonsense mutation in man.
1979,
Pubmed
Chang,
Suppression of the nonsense mutation in homozygous beta 0 thalassaemia.
1979,
Pubmed
Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed
Diamond,
Structure and properties of a bovine liver UGA suppressor serine tRNA with a tryptophan anticodon.
1981,
Pubmed
Geller,
A UGA termination suppression tRNATrp active in rabbit reticulocytes.
1980,
Pubmed
Gillam,
Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum ologodeoxyribonucleotide length.
1979,
Pubmed
Gillam,
Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: II. In vitro selection of mutant DNA.
1979,
Pubmed
Hatfield,
Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA.
1982,
Pubmed
Hatfield,
Characterization and nucleotide sequence of a chicken gene encoding an opal suppressor tRNA and its flanking DNA segments.
1983,
Pubmed
Hill,
Three different missense suppressor mutations affecting the tRNA GGG Gly species of Escherichia coli.
1974,
Pubmed
Hudziak,
Establishment of mammalian cell lines containing multiple nonsense mutations and functional suppressor tRNA genes.
1982,
Pubmed
,
Xenbase
Kisselev,
The role of the anticodon in recognition of tRNA by aminoacyl-tRNA synthetases.
1985,
Pubmed
Laski,
An amber suppressor tRNA gene derived by site-specific mutagenesis: cloning and function in mammalian cells.
1982,
Pubmed
,
Xenbase
Laski,
Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells.
1984,
Pubmed
,
Xenbase
Miller,
Genetic studies of the lac repressor. IX. Generation of altered proteins by the suppression of nonsence mutations.
1979,
Pubmed
O'Neill,
A human opal suppressor tRNA gene and pseudogene.
1985,
Pubmed
Rawlins,
Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis.
1980,
Pubmed
Roberts,
Molecular mechanism for missense suppression in E. coli.
1974,
Pubmed
Roberts,
Nucleotide sequence studies of normal and genetically altered glycine transfer ribonucleic acids from Escherichia coli.
1975,
Pubmed
Schulman,
Anticodon loop size and sequence requirements for recognition of formylmethionine tRNA by methionyl-tRNA synthetase.
1983,
Pubmed
Summers,
Functional suppression in mammalian cells of nonsense mutations in the herpes simplex virus thymidine kinase gene by suppressor tRNA genes.
1983,
Pubmed
Temple,
Construction of a functional human suppressor tRNA gene: an approach to gene therapy for beta-thalassaemia.
1982,
Pubmed
Yaniv,
A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG.
1974,
Pubmed
Young,
Measurement of suppressor transfer RNA activity.
1983,
Pubmed
