XB-ART-41515
J Am Chem Soc
2010 Jun 23;13224:8228-9. doi: 10.1021/ja906104e.
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Distance determination in proteins inside Xenopus laevis oocytes by double electron-electron resonance experiments.
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DEER (double electron-electron resonance) enables the observation of long-range dipole interactions (1.5-8 nm) between electron spin centers and has become a unique method for structural analysis of site-directed spin-labeled (SDSL) proteins. The method was applied to proteins inside eukaryotic cells, Xenopus laevis oocytes. DEER measurements of the oocytes, into which SDSL-ubiquitin derivates were injected, gave rise to interpretable signals and allowed us to perform in situ analyses of the interspin distances of the proteins.
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Species referenced: Xenopus laevis
Genes referenced: sdsl