Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
An upstream transcription factor, USF (MLTF), facilitates the formation of preinitiation complexes during in vitro chromatin assembly.
Workman JL
,
Roeder RG
,
Kingston RE
.
Abstract
During in vitro chromatin assembly the formation of transcription complexes is in direct competition with the assembly of promoter sequences into nucleosomes. Under these conditions the fold stimulation of transcription by an upstream transcription factor (USF) was greater than that observed in the absence of nucleosome assembly. Function of USF during nucleosome assembly required the simultaneous presence of the TATA box binding protein TFIID. Unlike TFIID, USF alone was unable to prevent repression of the promoter during nucleosome assembly. Furthermore, USF displayed reduced or no transcriptional stimulatory activity when added to previously assembled minichromosomes. Under conditions of nucleosome assembly, USF increased the number of assembled minichromosomes which contained stable preinitiation complexes. Subsequent to assembly, the rate at which preformed complexes initiated transcription appeared to be independent of the presence of USF. Thus USF potentiated the subsequent transcriptional activity of the promoter indirectly, apparently by increasing the rate or stability of TFIID binding. This activity resulted in the promoter becoming resistant to nucleosome mediated repression. These observations suggest that some ubiquitous upstream factors, e.g. USF, may play an important role in establishing the transcriptional potential of cellular genes during chromatin assembly.
Abmayr,
The pseudorabies immediate early protein stimulates in vitro transcription by facilitating TFIID: promoter interactions.
1988, Pubmed
Abmayr,
The pseudorabies immediate early protein stimulates in vitro transcription by facilitating TFIID: promoter interactions.
1988,
Pubmed
Almer,
Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements.
1986,
Pubmed
Buratowski,
Five intermediate complexes in transcription initiation by RNA polymerase II.
1989,
Pubmed
Cai,
Transcription initiation by RNA polymerase II in vitro. Properties of preinitiation, initiation, and elongation complexes.
1987,
Pubmed
Carcamo,
Factors involved in specific transcription by mammalian RNA polymerase II. Role of factors IID and MLTF in transcription from the adenovirus major late and IVa2 promoters.
1989,
Pubmed
Carthew,
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
1985,
Pubmed
Chodosh,
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
1986,
Pubmed
Clark-Adams,
Changes in histone gene dosage alter transcription in yeast.
1988,
Pubmed
Cordingley,
Steroid-dependent interaction of transcription factors with the inducible promoter of mouse mammary tumor virus in vivo.
1987,
Pubmed
Cromlish,
Transcriptionally active immediate-early protein of pseudorabies virus binds to specific sites on class II gene promoters.
1989,
Pubmed
Davison,
Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences.
1983,
Pubmed
Elgin,
The formation and function of DNase I hypersensitive sites in the process of gene activation.
1988,
Pubmed
Fire,
Interactions between RNA polymerase II, factors, and template leading to accurate transcription.
1984,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Greene,
Multiple basal elements of a human hsp70 promoter function differently in human and rodent cell lines.
1987,
Pubmed
Gross,
Nuclease hypersensitive sites in chromatin.
1988,
Pubmed
Han,
Depletion of histone H4 and nucleosomes activates the PHO5 gene in Saccharomyces cerevisiae.
1988,
Pubmed
Han,
Nucleosome loss activates yeast downstream promoters in vivo.
1988,
Pubmed
Hawley,
Separation and partial characterization of three functional steps in transcription initiation by human RNA polymerase II.
1985,
Pubmed
Hawley,
Functional steps in transcription initiation and reinitiation from the major late promoter in a HeLa nuclear extract.
1987,
Pubmed
Hen,
Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.
1982,
Pubmed
Kim,
Effects of histone H4 depletion on the cell cycle and transcription of Saccharomyces cerevisiae.
1988,
Pubmed
Knezetic,
Assembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates.
1988,
Pubmed
Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase
Ladiges,
The canine major histocompatibility complex. Supertypic specificities defined by the primed lymphocyte test (PLT).
1984,
Pubmed
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase
Laskey,
Nucleosome assembly.
1980,
Pubmed
,
Xenbase
Lorch,
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
1987,
Pubmed
Matsui,
Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
1980,
Pubmed
Matsui,
Transcription of adenovirus 2 major late and peptide IX genes under conditions of in vitro nucleosome assembly.
1987,
Pubmed
Miyamoto,
Stimulation of in vitro transcription by the upstream element of the adenovirus-2 major late promoter involves a specific factor.
1984,
Pubmed
Miyamoto,
Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter.
1985,
Pubmed
Moncollin,
Purification of a factor specific for the upstream element of the adenovirus-2 major late promoter.
1986,
Pubmed
Mueller,
Constitutive and metal-inducible protein:DNA interactions at the mouse metallothionein I promoter examined by in vivo and in vitro footprinting.
1988,
Pubmed
Pederson,
Core particle, fiber, and transcriptionally active chromatin structure.
1986,
Pubmed
Perlmann,
Specific glucocorticoid receptor binding to DNA reconstituted in a nucleosome.
1988,
Pubmed
Razvi,
A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA.
1983,
Pubmed
,
Xenbase
Reinberg,
Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site.
1987,
Pubmed
Richard-Foy,
Sequence-specific positioning of nucleosomes over the steroid-inducible MMTV promoter.
1987,
Pubmed
Rougvie,
The RNA polymerase II molecule at the 5' end of the uninduced hsp70 gene of D. melanogaster is transcriptionally engaged.
1988,
Pubmed
Saltzman,
Promoter specificity and modulation of RNA polymerase II transcription.
1989,
Pubmed
Samuels,
Separation and characterization of factors mediating accurate transcription by RNA polymerase II.
1982,
Pubmed
Sawadogo,
Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei.
1988,
Pubmed
Sawadogo,
Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
1985,
Pubmed
Sawadogo,
Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
1985,
Pubmed
Sawadogo,
Multiple forms of the human gene-specific transcription factor USF. II. DNA binding properties and transcriptional activity of the purified HeLa USF.
1988,
Pubmed
Taylor,
Factor substitution in a human HSP70 gene promoter: TATA-dependent and TATA-independent interactions.
1990,
Pubmed
Thomas,
Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter.
1988,
Pubmed
Van Dyke,
Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
1988,
Pubmed
Van Dyke,
Stability of transcription complexes on class II genes.
1989,
Pubmed
Weintraub,
Assembly and propagation of repressed and depressed chromosomal states.
1985,
Pubmed
Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
,
Xenbase
Workman,
Transcriptional regulation by the immediate early protein of pseudorabies virus during in vitro nucleosome assembly.
1988,
Pubmed
,
Xenbase
Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase
Wu,
Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.
,
Pubmed
Yu,
Generation and functional analyses for base-substitution mutants of the adenovirus 2 major late promoter.
1984,
Pubmed
