Differentiation 1985 Jan 01;301:29-34. doi: 10.1111/j.1432-0436.1985.tb00509.x.

Anti-immunoglobulin M induces both B-lymphocyte proliferation and differentiation in Xenopus laevis.

Abstract
Anti-IgM induced the proliferation of spleen lymphocytes of the amphibian Xenopus laevis as determined by 3H-thymidine uptake. The responding cells were B lymphocytes, since lymphocyte populations enriched in surface-Ig-positive cells exhibited an increased proliferative response, and spleen cells from larvally thymectomized animals still responded to anti-IgM. Immunofluorescence analysis and gel electrophoresis of biosynthetically labeled Ig polypeptides revealed that lymphoblasts induced by anti-IgM differentiated into plasmablasts that synthesized and secreted mainly IgM and small amounts of IgY. The in vitro differentiation of B lymphocytes also occurred in spleen cells obtained from thymectomized animals. These findings are in contrast with those obtained in mammals and suggest that the differentiation of B lymphocytes in X. laevis is subject to different regulatory mechanisms.

PubMed ID: 3879229
Article link: Differentiation


Species referenced: Xenopus laevis
Genes referenced: ighx