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Receptive range analysis of a mouse odorant receptor subfamily.
Li J
,
Haddad R
,
Santos V
,
Bavan S
,
Luetje CW
.
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Mammals deploy a large array of odorant receptors (ORs) to detect and distinguish a vast number of odorant molecules. ORs vary widely in the type of odorant structures recognized and in the breadth of molecular receptive range (MRR), with some ORs recognizing a small group of closely related molecules and other ORs recognizing a wide range of structures. While closely related ORs have been shown to have similar MRRs, the functional relationships among less closely related ORs are unclear. We screened a small group of ORs with a diverse odorant panel to identify a new odorant-OR pairing (unsaturated aldehydes and MOR263-3). We then extensively screened MOR263-3 and a series of additional MORs related to MOR263-3 in various ways. MORs related by phylogenetic analysis (several other members of the MOR263 subfamily) had MRRs that overlapped with the MRR of MOR263-3, even with amino acid identity as low as 48% (MOR263-2). MOR171-17, predicted to be functionally related to MOR263-3 by an alternative bioinformatic analysis, but with only 39% amino acid identity, had a distinct odorant specificity. Our results support the use of phylogenetic analysis to predict functional relationships among ORs with relatively low amino acid identity. We screened a small group of mouse odorant receptors (MORs) with a diverse odorant panel to identify a new odorant-OR pairing (unsaturated aldehydes and MOR263-3), then extensively screened a series of additional MORs related to MOR263-3 in various ways. MORs related by phylogenetic analysis had odorant specificities that overlapped with that of MOR263-3, but MOR171-17, predicted to be functionally related to MOR263-3 by an alternative bioinformatic analysis, had a distinct odorant specificity.
Abaffy,
Functional analysis of a mammalian odorant receptor subfamily.
2006, Pubmed,
Xenbase
Abaffy,
Functional analysis of a mammalian odorant receptor subfamily.
2006,
Pubmed
,
Xenbase
Araneda,
The molecular receptive range of an odorant receptor.
2000,
Pubmed
Bakalyar,
Identification of a specialized adenylyl cyclase that may mediate odorant detection.
1990,
Pubmed
Bozza,
Odorant receptor expression defines functional units in the mouse olfactory system.
2002,
Pubmed
Buck,
A novel multigene family may encode odorant receptors: a molecular basis for odor recognition.
1991,
Pubmed
Feinstein,
A contextual model for axonal sorting into glomeruli in the mouse olfactory system.
2004,
Pubmed
Godfrey,
The mouse olfactory receptor gene family.
2004,
Pubmed
Grosmaitre,
SR1, a mouse odorant receptor with an unusually broad response profile.
2009,
Pubmed
Haddad,
A metric for odorant comparison.
2008,
Pubmed
Haddad,
Global features of neural activity in the olfactory system form a parallel code that predicts olfactory behavior and perception.
2010,
Pubmed
Jones,
Golf: an olfactory neuron specific-G protein involved in odorant signal transduction.
1989,
Pubmed
Kajiya,
Molecular bases of odor discrimination: Reconstitution of olfactory receptors that recognize overlapping sets of odorants.
2001,
Pubmed
Krautwurst,
Identification of ligands for olfactory receptors by functional expression of a receptor library.
1998,
Pubmed
Li,
A broadly tuned mouse odorant receptor that detects nitrotoluenes.
2012,
Pubmed
Lowe,
Nonlinear amplification by calcium-dependent chloride channels in olfactory receptor cells.
1993,
Pubmed
Ma,
High-affinity activators of cystic fibrosis transmembrane conductance regulator (CFTR) chloride conductance identified by high-throughput screening.
2002,
Pubmed
Malnic,
Combinatorial receptor codes for odors.
1999,
Pubmed
Man,
Prediction of the odorant binding site of olfactory receptor proteins by human-mouse comparisons.
2004,
Pubmed
Man,
A framework for exploring functional variability in olfactory receptor genes.
2007,
Pubmed
Mombaerts,
Genes and ligands for odorant, vomeronasal and taste receptors.
2004,
Pubmed
Mori,
The olfactory bulb: coding and processing of odor molecule information.
1999,
Pubmed
Nakamura,
A cyclic nucleotide-gated conductance in olfactory receptor cilia.
,
Pubmed
Nara,
A large-scale analysis of odor coding in the olfactory epithelium.
2011,
Pubmed
Oka,
Olfactory receptor antagonism between odorants.
2004,
Pubmed
Oka,
Odorant receptor map in the mouse olfactory bulb: in vivo sensitivity and specificity of receptor-defined glomeruli.
2006,
Pubmed
Peterlin,
The importance of odorant conformation to the binding and activation of a representative olfactory receptor.
2008,
Pubmed
Reed,
After the holy grail: establishing a molecular basis for Mammalian olfaction.
2004,
Pubmed
Repicky,
Molecular receptive range variation among mouse odorant receptors for aliphatic carboxylic acids.
2009,
Pubmed
,
Xenbase
Saito,
RTP family members induce functional expression of mammalian odorant receptors.
2004,
Pubmed
Saito,
Odor coding by a Mammalian receptor repertoire.
2009,
Pubmed
Schmiedeberg,
Structural determinants of odorant recognition by the human olfactory receptors OR1A1 and OR1A2.
2007,
Pubmed
Schultz,
Pharmacology of CFTR chloride channel activity.
1999,
Pubmed
Serizawa,
Negative feedback regulation ensures the one receptor-one olfactory neuron rule in mouse.
2003,
Pubmed
Touhara,
Functional identification and reconstitution of an odorant receptor in single olfactory neurons.
1999,
Pubmed
Uezono,
Receptors that couple to 2 classes of G proteins increase cAMP and activate CFTR expressed in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Von Dannecker,
Ric-8B promotes functional expression of odorant receptors.
2006,
Pubmed
Wetzel,
Specificity and sensitivity of a human olfactory receptor functionally expressed in human embryonic kidney 293 cells and Xenopus Laevis oocytes.
1999,
Pubmed
,
Xenbase
Young,
Different evolutionary processes shaped the mouse and human olfactory receptor gene families.
2002,
Pubmed
Zhang,
High-throughput microarray detection of olfactory receptor gene expression in the mouse.
2004,
Pubmed
Zhang,
The olfactory receptor gene superfamily of the mouse.
2002,
Pubmed
Zhuang,
Synergism of accessory factors in functional expression of mammalian odorant receptors.
2007,
Pubmed
