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The role of the beta 4-subunit in determining the kinetic properties of rat neuronal nicotinic acetylcholine alpha 3-receptors.
Papke RL
,
Heinemann SF
.
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1. Single-channel currents were recorded from Xenopus oocytes which had been injected with complementary RNAs (cRNAs) for the neuronal nicotinic acetylcholine receptor subunits alpha 3 and beta 4. The co-expression of alpha 3 and beta 4 gave rise to three different channel types with conductances of 22, 18 and 13 pS. 2. The activity arising from the expression of these two subunits was compared with that observed when the alpha 3 subunit was co-expressed with an alternative beta-subunit, beta 2. The alpha 3 beta 4-receptors differed from alpha 3 beta 2-receptors in conductance, open times and most notably, in burst kinetics. The association of the beta 4-subunit with the alpha 3-subunit results in receptors which have a high probability of re-opening after closing, yielding protracted bursts of activity not observed when the alpha 3-subunit is associated with the beta 2-subunit. 3. All three alpha 3 beta 4-channel types had similar burst kinetics. However, the 13 pS conductance channels showed an additional long-lived open state to varying degrees when clusters of activity within an individual record were examined.
Blount,
Molecular basis of the two nonequivalent ligand binding sites of the muscle nicotinic acetylcholine receptor.
1989, Pubmed
Blount,
Molecular basis of the two nonequivalent ligand binding sites of the muscle nicotinic acetylcholine receptor.
1989,
Pubmed
Bormann,
Three types of acetylcholine-induced single channel currents in clonal rat pheochromocytoma cells.
1983,
Pubmed
Boulter,
Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family.
1987,
Pubmed
,
Xenbase
Boulter,
Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor alpha-subunit.
,
Pubmed
Clapham,
Substance P reduces acetylcholine-induced currents in isolated bovine chromaffin cells.
1984,
Pubmed
Colquhoun,
Fast events in single-channel currents activated by acetylcholine and its analogues at the frog muscle end-plate.
1985,
Pubmed
Connolly,
Structure-function relationships in nicotinic acetylcholine receptors.
1989,
Pubmed
Couturier,
A neuronal nicotinic acetylcholine receptor subunit (alpha 7) is developmentally regulated and forms a homo-oligomeric channel blocked by alpha-BTX.
1990,
Pubmed
,
Xenbase
Couturier,
Alpha 5, alpha 3, and non-alpha 3. Three clustered avian genes encoding neuronal nicotinic acetylcholine receptor-related subunits.
1990,
Pubmed
,
Xenbase
Deneris,
Primary structure and expression of beta 2: a novel subunit of neuronal nicotinic acetylcholine receptors.
1988,
Pubmed
,
Xenbase
Duvoisin,
The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: beta 4.
1989,
Pubmed
,
Xenbase
Fenwick,
A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine.
1982,
Pubmed
Goldman,
Members of a nicotinic acetylcholine receptor gene family are expressed in different regions of the mammalian central nervous system.
1987,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Jaramillo,
Kinetic differences between embryonic- and adult-type acetylcholine receptors in rat myotubes.
1988,
Pubmed
Labarca,
The occurrence of long openings in the purified cholinergic receptor channel increases with acetylcholine concentration.
1985,
Pubmed
Lo,
Influence of the gamma subunit and expression system on acetylcholine receptor gating.
1990,
Pubmed
,
Xenbase
Luetje,
Both alpha- and beta-subunits contribute to the agonist sensitivity of neuronal nicotinic acetylcholine receptors.
1991,
Pubmed
,
Xenbase
Moss,
Functional properties and developmental regulation of nicotinic acetylcholine receptors on embryonic chicken sympathetic neurons.
1989,
Pubmed
Papke,
Single-channel currents of rat neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Papke,
Mechanisms of noncompetitive inhibition of acetylcholine-induced single-channel currents.
1989,
Pubmed
Pedersen,
d-Tubocurarine binding sites are located at alpha-gamma and alpha-delta subunit interfaces of the nicotinic acetylcholine receptor.
1990,
Pubmed
Sigworth,
Data transformations for improved display and fitting of single-channel dwell time histograms.
1987,
Pubmed
Sine,
Activation of acetylcholine receptors on clonal mammalian BC3H-1 cells by low concentrations of agonist.
1986,
Pubmed
Sine,
Acetylcholine receptor activation by a site-selective ligand: nature of brief open and closed states in BC3H-1 cells.
1986,
Pubmed
Sine,
Activation of a nicotinic acetylcholine receptor.
1984,
Pubmed
Takeda,
A patch-clamp study of the partial agonist actions of tubocurarine on rat myotubes.
1984,
Pubmed
Wada,
Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor.
1988,
Pubmed
,
Xenbase
Wada,
Distribution of alpha 2, alpha 3, alpha 4, and beta 2 neuronal nicotinic receptor subunit mRNAs in the central nervous system: a hybridization histochemical study in the rat.
1989,
Pubmed
,
Xenbase
