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Hydrogen peroxide stimulates the epithelial sodium channel through a phosphatidylinositide 3-kinase-dependent pathway.
Ma HP
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Recent studies indicate that oxidative stress mediates salt-sensitive hypertension. To test the hypothesis that the renal epithelial sodium channel (ENaC) is a target of oxidative stress, patch clamp techniques were used to determine whether ENaC in A6 distalnephron cells is regulated by hydrogen peroxide (H(2)O(2)). In the cell-attached configuration, H(2)O(2) significantly increased ENaC open probability (P(o)) and single-channel current amplitude but not the unit conductance. High concentrations of exogenous H(2)O(2) are required to elevate intracellular H(2)O(2), probably because catalase, the enzyme that promotes the decomposition of H(2)O(2) to H(2)O and O(2), is highly expressed in A6 cells. The effect of H(2)O(2) on ENaC P(o) was enhanced by 3-aminotriazole, a catalase inhibitor, and abolished by overexpression of catalase, indicating that intracellular H(2)O(2) levels are critical to produce the effect. However, H(2)O(2) did not directly activate ENaC in inside-out patches. The effects of H(2)O(2) on ENaC P(o) and amiloride-sensitive Na(+) current were abolished by inhibition of phosphatidylinositide 3-kinase (PI3K). Confocal microscopy data showed that H(2)O(2) elevated phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P(3)) in the apical membrane by stimulating PI3K. Because ENaC is stimulated by PI(3,4,5)P(3), these data suggest that H(2)O(2) stimulates ENaC via PI3K-mediated increases in apical PI(3,4,5)P(3).
Abe,
Effect of sodium delivery on superoxide and nitric oxide in the medullary thick ascending limb.
2006, Pubmed
Abe,
Effect of sodium delivery on superoxide and nitric oxide in the medullary thick ascending limb.
2006,
Pubmed
Avshalumov,
Activation of ATP-sensitive K+ (K(ATP)) channels by H2O2 underlies glutamate-dependent inhibition of striatal dopamine release.
2003,
Pubmed
Bao,
Ceramide mediates inhibition of the renal epithelial sodium channel by tumor necrosis factor-alpha through protein kinase C.
2007,
Pubmed
,
Xenbase
Barlow,
H(2)O(2) opens BK(Ca) channels via the PLA(2)-arachidonic acid signaling cascade in coronary artery smooth muscle.
2000,
Pubmed
Bełtowski,
H2O2 and Src-dependent transactivation of the EGF receptor mediates the stimulatory effect of leptin on renal ERK and Na+, K+-ATPase.
2006,
Pubmed
Bienert,
Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes.
2007,
Pubmed
Blazer-Yost,
Real-time three-dimensional imaging of lipid signal transduction: apical membrane insertion of epithelial Na(+) channels.
2004,
Pubmed
,
Xenbase
Chen,
Increased H(2)O(2) counteracts the vasodilator and natriuretic effects of superoxide dismutation by tempol in renal medulla.
2003,
Pubmed
Cuevas,
Tyrosine phosphorylation of p85 relieves its inhibitory activity on phosphatidylinositol 3-kinase.
2001,
Pubmed
Gamper,
Oxidative modification of M-type K(+) channels as a mechanism of cytoprotective neuronal silencing.
2006,
Pubmed
Giannoni,
Intracellular reactive oxygen species activate Src tyrosine kinase during cell adhesion and anchorage-dependent cell growth.
2005,
Pubmed
Gill,
NADPH oxidases in the kidney.
2006,
Pubmed
Kitiyakara,
Salt intake, oxidative stress, and renal expression of NADPH oxidase and superoxide dismutase.
2003,
Pubmed
Lin,
Hydrogen peroxide-induced Ca2+ mobilization in pulmonary arterial smooth muscle cells.
2007,
Pubmed
Ma,
Regulation of the epithelial sodium channel by phosphatidylinositides: experiments, implications, and speculations.
2007,
Pubmed
Ma,
Anionic phospholipids regulate native and expressed epithelial sodium channel (ENaC).
2002,
Pubmed
,
Xenbase
Martin-Belmonte,
PTEN-mediated apical segregation of phosphoinositides controls epithelial morphogenesis through Cdc42.
2007,
Pubmed
Marunaka,
Effects of vasopressin and cAMP on single amiloride-blockable Na channels.
1991,
Pubmed
Mori,
Enhanced superoxide production in renal outer medulla of Dahl salt-sensitive rats reduces nitric oxide tubular-vascular cross-talk.
2007,
Pubmed
Nisticò,
Neuroprotective effect of hydrogen peroxide on an in vitro model of brain ischaemia.
2008,
Pubmed
Otsu,
Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase.
1991,
Pubmed
Pech,
Pendrin modulates ENaC function by changing luminal HCO3-.
2010,
Pubmed
,
Xenbase
Pochynyuk,
Binding and direct activation of the epithelial Na+ channel (ENaC) by phosphatidylinositides.
2007,
Pubmed
Pochynyuk,
Regulation of the epithelial Na+ channel (ENaC) by phosphatidylinositides.
2006,
Pubmed
Rhee,
Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins.
2005,
Pubmed
Rhee,
Controlled elimination of intracellular H(2)O(2): regulation of peroxiredoxin, catalase, and glutathione peroxidase via post-translational modification.
2005,
Pubmed
Taylor,
Effect of renal medullary H2O2 on salt-induced hypertension and renal injury.
2005,
Pubmed
Taylor,
NADPH oxidase in the renal medulla causes oxidative stress and contributes to salt-sensitive hypertension in Dahl S rats.
2006,
Pubmed
Veal,
Hydrogen peroxide sensing and signaling.
2007,
Pubmed
Wang,
Cyclosporine stimulates the renal epithelial sodium channel by elevating cholesterol.
2009,
Pubmed
,
Xenbase
Warnock,
The epithelial sodium channel in hypertension.
1999,
Pubmed
Warnock,
Liddle syndrome: genetics and mechanisms of Na+ channel defects.
2001,
Pubmed
Williams,
Advances in genetic hypertension.
2007,
Pubmed
Yu,
Aldosterone-induced increases in superoxide production counters nitric oxide inhibition of epithelial Na channel activity in A6 distal nephron cells.
2007,
Pubmed
,
Xenbase
Yu,
Single-channel analysis of functional epithelial sodium channel (ENaC) stability at the apical membrane of A6 distal kidney cells.
2008,
Pubmed
,
Xenbase
Zhang,
Regulation of TRP channel TRPM2 by the tyrosine phosphatase PTPL1.
2007,
Pubmed
Zhang,
Anionic phospholipids differentially regulate the epithelial sodium channel (ENaC) by interacting with alpha, beta, and gamma ENaC subunits.
2010,
Pubmed
Zhang,
Vascular hypertrophy in angiotensin II-induced hypertension is mediated by vascular smooth muscle cell-derived H2O2.
2005,
Pubmed
