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Shaker potassium channel gating. II: Transitions in the activation pathway.
Zagotta WN
,
Hoshi T
,
Dittman J
,
Aldrich RW
.
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Voltage-dependent gating behavior of Shaker potassium channels without N-type inactivation (ShB delta 6-46) expressed in Xenopus oocytes was studied. The voltage dependence of the steady-state open probability indicated that the activation process involves the movement of the equivalent of 12-16 electronic charges across the membrane. The sigmoidal kinetics of the activation process, which is maintained at depolarized voltages up to at least +100 mV indicate the presence of at least five sequential conformational changes before opening. The voltage dependence of the gating charge movement suggested that each elementary transition involves 3.5 electronic charges. The voltage dependence of the forward opening rate, as estimated by the single-channel first latency distribution, the final phase of the macroscopic ionic current activation, the ionic current reactivation and the ON gating current time course, showed movement of the equivalent of 0.3 to 0.5 electronic charges were associated with a large number of the activation transitions. The equivalent charge movement of 1.1 electronic charges was associated with the closing conformational change. The results were generally consistent with models involving a number of independent and identical transitions with a major exception that the first closing transition is slower than expected as indicated by tail current and OFF gating charge measurements.
Almers,
Gating currents and charge movements in excitable membranes.
1978, Pubmed
Almers,
Gating currents and charge movements in excitable membranes.
1978,
Pubmed
Andersen,
Molecular determinants of channel function.
1992,
Pubmed
Armstrong,
Charge movement associated with the opening and closing of the activation gates of the Na channels.
1974,
Pubmed
Armstrong,
Sodium channels and gating currents.
1981,
Pubmed
Ascher,
Life time and elementary conductance of the channels mediating the excitatory effects of acetylcholine in Aplysia neurones.
1978,
Pubmed
Bezanilla,
Molecular basis of gating charge immobilization in Shaker potassium channels.
1991,
Pubmed
,
Xenbase
COLE,
Potassium ion current in the squid giant axon: dynamic characteristic.
1960,
Pubmed
Cahalan,
A voltage-gated potassium channel in human T lymphocytes.
1985,
Pubmed
Durell,
Atomic scale structure and functional models of voltage-gated potassium channels.
1992,
Pubmed
Gautam,
Alteration of potassium channel gating: molecular analysis of the Drosophila Sh5 mutation.
1990,
Pubmed
,
Xenbase
Gilly,
Gating current and potassium channels in the giant axon of the squid.
1980,
Pubmed
Gilly,
Divalent cations and the activation kinetics of potassium channels in squid giant axons.
1982,
Pubmed
Greenblatt,
The structure of the voltage-sensitive sodium channel. Inferences derived from computer-aided analysis of the Electrophorus electricus channel primary structure.
1985,
Pubmed
Guy,
Molecular model of the action potential sodium channel.
1986,
Pubmed
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
Hill,
On the theory of ion transport across the nerve membrane. II. Potassium ion kinetics and cooperativity (with x = 4).
1971,
Pubmed
Hill,
On the theory of ion transport across the nerve membrane. 3. Potassium ion kinetics and cooperativity (with x=4,6,9).
1971,
Pubmed
Hoshi,
Shaker potassium channel gating. I: Transitions near the open state.
1994,
Pubmed
,
Xenbase
Hurst,
Cooperative interactions among subunits of a voltage-dependent potassium channel. Evidence from expression of concatenated cDNAs.
1992,
Pubmed
,
Xenbase
Iverson,
A-type potassium channels expressed from Shaker locus cDNA.
1988,
Pubmed
,
Xenbase
Kamb,
Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity.
1988,
Pubmed
Koren,
Gating mechanism of a cloned potassium channel expressed in frog oocytes and mammalian cells.
1990,
Pubmed
,
Xenbase
Lichtinghagen,
Molecular basis of altered excitability in Shaker mutants of Drosophila melanogaster.
1990,
Pubmed
,
Xenbase
Liman,
Voltage-sensing residues in the S4 region of a mammalian K+ channel.
1991,
Pubmed
,
Xenbase
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Lopez,
Hydrophobic substitution mutations in the S4 sequence alter voltage-dependent gating in Shaker K+ channels.
1991,
Pubmed
,
Xenbase
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
Marchais,
Interaction of permeant ions with channels activated by acetylcholine in Aplysia neurones.
1979,
Pubmed
Matteson,
External monovalent cations that impede the closing of K channels.
1986,
Pubmed
McCormack,
A role for hydrophobic residues in the voltage-dependent gating of Shaker K+ channels.
1991,
Pubmed
,
Xenbase
Noda,
Expression of functional sodium channels from cloned cDNA.
,
Pubmed
,
Xenbase
Oxford,
Some kinetic and steady-state properties of sodium channels after removal of inactivation.
1981,
Pubmed
Papazian,
Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence.
1991,
Pubmed
,
Xenbase
Perozo,
Gating currents in Shaker K+ channels. Implications for activation and inactivation models.
1992,
Pubmed
,
Xenbase
Pongs,
Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.
1988,
Pubmed
Rojas,
On the relation between displacement currents and activation of the sodium conductance in the squid giant axon.
1975,
Pubmed
Sala,
Voltage-dependent slowing of K channel closing kinetics by Rb+.
1991,
Pubmed
Schoppa,
The size of gating charge in wild-type and mutant Shaker potassium channels.
1992,
Pubmed
Shapiro,
Selectivity and gating of the type L potassium channel in mouse lymphocytes.
1991,
Pubmed
Shapiro,
Permeant ion effects on the gating kinetics of the type L potassium channel in mouse lymphocytes.
1991,
Pubmed
Spruce,
Rubidium ions and the gating of delayed rectifier potassium channels of frog skeletal muscle.
1989,
Pubmed
Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
Pubmed
,
Xenbase
Stühmer,
Gating currents of inactivating and non-inactivating potassium channels expressed in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Swenson,
K+ channels close more slowly in the presence of external K+ and Rb+.
1981,
Pubmed
Taglialatela,
Novel voltage clamp to record small, fast currents from ion channels expressed in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Taglialatela,
Gating currents from a delayed rectifier K+ channel with altered pore structure and function.
1992,
Pubmed
,
Xenbase
Taylor,
Sodium and gating current time shifts resulting from changes in initial conditions.
1983,
Pubmed
Tempel,
Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila.
1987,
Pubmed
Timpe,
Four cDNA clones from the Shaker locus of Drosophila induce kinetically distinct A-type potassium currents in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Tytgat,
Evidence for cooperative interactions in potassium channel gating.
1992,
Pubmed
,
Xenbase
Vandenberg,
A sodium channel gating model based on single channel, macroscopic ionic, and gating currents in the squid giant axon.
1991,
Pubmed
White,
Activation of squid axon K+ channels. Ionic and gating current studies.
1985,
Pubmed
Zagotta,
Voltage-dependent gating of Shaker A-type potassium channels in Drosophila muscle.
1990,
Pubmed
Zagotta,
Alterations in activation gating of single Shaker A-type potassium channels by the Sh5 mutation.
1990,
Pubmed
Zagotta,
Shaker potassium channel gating. III: Evaluation of kinetic models for activation.
1994,
Pubmed
,
Xenbase