Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1990 Dec 01;913:4399-407. doi: 10.1002/j.1460-2075.1990.tb07890.x.
Show Gene links
Show Anatomy links
Molecular basis of altered excitability in Shaker mutants of Drosophila melanogaster.
Lichtinghagen R
,
Stocker M
,
Wittka R
,
Boheim G
,
Stühmer W
,
Ferrus A
,
Pongs O
.
???displayArticle.abstract???
Mutations in the Shaker (Sh) locus of Drosophila melanogaster have differing effects on action potential duration and repolarization in neurons as well as on A-type K+ channels (IA) in muscle. The molecular basis of three exemplary Sh alleles (ShKS133, ShE62 and Sh5) has been identified. They are point mutations in the Sh transcription unit expressing aberrant voltage-gated A-type K+ channels. Replicas of each mutation have been introduced by in vitro mutagenesis into Sh cDNA. The expression of in vitro transcribed mutant Sh cRNA in Xenopus laevis oocytes reproduced the specific phenotypic traits of each Sh allele. The lack of IA in ShKS133 is due to a missense mutation within a sequence motif occurring in all hitherto characterized voltage-gated K+ channel forming proteins. The reduction of IA in ShE62 is due to a mutation in an AG acceptor site. The intervening sequence between exons 19 and 20 is not spliced in ShE62 RNA. As a consequence, ShE62 flies do not contain the full complement of Sh K+ forming proteins. Finally, the Sh5 mutation leads to an altered voltage dependence of K+ channel activation and inactivation as well as to an accelerated rate of recovery from inactivation. This is due to a missense mutation altering the amino acid sequence of the proposed transmembrane segment S5 of the Sh K+ channels. Segment S5 is located adjacently to the presumed voltage sensor of voltage-gated ion channels. The results explain the altered properties of excitable cells in Sh mutants and provide a general model for the possible role of A-type K+ channels in modulating action potential profiles.
Abel,
Gene regulation. Action of leucine zippers.
1989, Pubmed
Abel,
Gene regulation. Action of leucine zippers.
1989,
Pubmed
Abrams,
Is contiguity detection in classical conditioning a system or a cellular property? Learning in Aplysia suggests a possible molecular site.
1988,
Pubmed
Aebi,
Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA.
1986,
Pubmed
Barbas,
Antibodies against Drosophila potassium channels identify membrane proteins across species.
1989,
Pubmed
Butler,
A family of putative potassium channel genes in Drosophila.
1989,
Pubmed
Crow,
Cellular and molecular analysis of associative learning and memory in Hermissenda.
1988,
Pubmed
Ferrús,
Genetic analysis of the Shaker gene complex of Drosophila melanogaster.
1990,
Pubmed
Frech,
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
1989,
Pubmed
,
Xenbase
Gisselmann,
The interference of truncated with normal potassium channel subunits leads to abnormal behaviour in transgenic Drosophila melanogaster.
1989,
Pubmed
Haugland,
A voltage-clamp analysis of gene-dosage effects of the Shaker locus on larval muscle potassium currents in Drosophila.
1990,
Pubmed
Iverson,
A-type potassium channels expressed from Shaker locus cDNA.
1988,
Pubmed
,
Xenbase
Kamb,
Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity.
1988,
Pubmed
Kamb,
Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel.
1987,
Pubmed
Kramer,
The gapped duplex DNA approach to oligonucleotide-directed mutation construction.
1984,
Pubmed
Landschulz,
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
1988,
Pubmed
MacKinnon,
Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor.
1989,
Pubmed
,
Xenbase
Mount,
A catalogue of splice junction sequences.
1982,
Pubmed
Pongs,
Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.
1988,
Pubmed
Reed,
The organization of 3' splice-site sequences in mammalian introns.
1989,
Pubmed
Reed,
Intron sequences involved in lariat formation during pre-mRNA splicing.
1985,
Pubmed
Saiki,
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
1985,
Pubmed
Salkoff,
Genetic and voltage-clamp analysis of a Drosophila potassium channel.
1983,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schwarz,
Multiple potassium-channel components are produced by alternative splicing at the Shaker locus in Drosophila.
1988,
Pubmed
Solc,
Single-channel and genetic analyses reveal two distinct A-type potassium channels in Drosophila.
1987,
Pubmed
Steller,
A transposable P vector that confers selectable G418 resistance to Drosophila larvae.
1985,
Pubmed
Stühmer,
Potassium channels expressed from rat brain cDNA have delayed rectifier properties.
1988,
Pubmed
,
Xenbase
Stühmer,
Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
1989,
Pubmed
,
Xenbase
Stühmer,
Patch clamp characterization of sodium channels expressed from rat brain cDNA.
1987,
Pubmed
,
Xenbase
Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
Pubmed
,
Xenbase
Tanouye,
Action potentials in normal and Shaker mutant Drosophila.
1985,
Pubmed
Tanouye,
Abnormal action potentials associated with the Shaker complex locus of Drosophila.
1981,
Pubmed
Timpe,
Gene dosage and complementation analysis of the Shaker locus in Drosophila.
1987,
Pubmed
Timpe,
Four cDNA clones from the Shaker locus of Drosophila induce kinetically distinct A-type potassium currents in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Timpe,
Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Wu,
Voltage clamp analysis of membrane currents in larval muscle fibers of Drosophila: alteration of potassium currents in Shaker mutants.
1985,
Pubmed
Yokoyama,
Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells. Primary structure and functional expression from cDNAs.
1989,
Pubmed
,
Xenbase
Zagotta,
Alterations in activation gating of single Shaker A-type potassium channels by the Sh5 mutation.
1990,
Pubmed