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Point mutational analysis of the Xenopus laevis 5S gene promoter.
Pieler T
,
Appel B
,
Oei SL
,
Mentzel H
,
Erdmann VA
.
Abstract
We have introduced C to T transitions into GC and CG base pairs of the Xenopus laevis somatic 5S gene coding region and its 5' flank in order to analyse their effects on transcription activity and regulation. These studies allow us to differentiate between the two promoter elements and their spacer, within the internal control region. Mutations within the 5' element which dissent from the corresponding tRNA consensus sequence reduce transcription activity substantially without significantly affecting transcription factor (TF) III A binding. Mutations in the spacer region have no pronounced effect on transcription. The 3' promoter element is found to extend to position 97, since mutations in this region interfere with transcription activity. This may be, at least partially, attributable to a reduced competition strength for TF III A.
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978, Pubmed,
Xenbase
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase
Ciliberto,
Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
1983,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Erdmann,
Collection of published 5S and 5.8S ribosomal RNA sequences.
1984,
Pubmed
Folk,
A detailed mutational analysis of the eucaryotic tRNAmet1 gene promoter.
1983,
Pubmed
,
Xenbase
Ford,
Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Galli,
Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
1981,
Pubmed
,
Xenbase
Gargiulo,
Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors.
1984,
Pubmed
,
Xenbase
Hipskind,
5'-flanking sequences that inhibit in vitro transcription of a xenopus laevis tRNA gene.
1983,
Pubmed
,
Xenbase
Hofstetter,
A split promoter for a eucaryotic tRNA gene.
1981,
Pubmed
,
Xenbase
Ladiges,
The canine major histocompatibility complex. Supertypic specificities defined by the primed lymphocyte test (PLT).
1984,
Pubmed
Messing,
New M13 vectors for cloning.
1983,
Pubmed
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Pieler,
Three-dimensional structural model of eubacterial 5S RNA that has functional implications.
1982,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sakonju,
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
1981,
Pubmed
,
Xenbase
Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schlissel,
The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
1984,
Pubmed
,
Xenbase
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Sharp,
Internal control regions for transcription of eukaryotic tRNA genes.
1981,
Pubmed
,
Xenbase
Shastry,
Multiple factors involved in the transcription of class III genes in Xenopus laevis.
1982,
Pubmed
,
Xenbase
Shortle,
Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.
1978,
Pubmed
Wakefield,
Cytoplasmic regulation of 5S RNA genes in nuclear-transplant embryos.
1983,
Pubmed
,
Xenbase
Wormington,
A quantitative assay for Xenopus 5S RNA gene transcription in vitro.
1981,
Pubmed
,
Xenbase
