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Abstract
We isolated and characterized Xenopus laevis c-myc cDNAs from an oocyte-specific library. These cDNA clones encompass 2.35 kilobases of the X. laevis c-myc RNA and contain the entire coding domain of 1,257 nucleotides of the 419-amino acid-long X. laevis c-myc protein. The 2.7-kilobase X. laevis c-myc mRNA is expressed in the oocyte, maintained in the egg, and is present throughout the early cleavage stages of embryogenesis. At the time of transcriptional activation in the embryo the c-myc RNA levels show a significant decline and then reaccumulate continuously throughout the remainder of premorphogenic development. At the early neurula stage of embryogenesis the pattern of c-myc RNA expression is elevated in the mesoderm with respect to the endoderm and ectoderm. In the adult X. laevis the c-myc mRNA is expressed in some (e.g., skin, muscle) but not all differentiated tissues. The X. laevis c-myc protein migrates as a doublet of 61,000- and 64,000-dalton species. Both species are phosphorylated in oocytes and somatic cells, exhibit extremely short half-lives of less than 30 min, and are localized to the nuclear fraction of somatic cells. By contrast, the oocyte protein shows both cytoplasmic and germinal vesicle distribution and appears to be stable.
Abrams,
Nuclear location of the putative transforming protein of avian myelocytomatosis virus.
1982, Pubmed
Abrams,
Nuclear location of the putative transforming protein of avian myelocytomatosis virus.
1982,
Pubmed
Battey,
The human c-myc oncogene: structural consequences of translocation into the IgH locus in Burkitt lymphoma.
1983,
Pubmed
Bernard,
Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours.
1983,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Blanchard,
c-myc gene is transcribed at high rate in G0-arrested fibroblasts and is post-transcriptionally regulated in response to growth factors.
,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Colby,
Identification and nucleotide sequence of a human locus homologous to the v-myc oncogene of avian myelocytomatosis virus MC29.
1983,
Pubmed
Coppola,
Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment.
,
Pubmed
Cory,
Variant (6;15) translocations in murine plasmacytomas involve a chromosome 15 locus at least 72 kb from the c-myc oncogene.
1985,
Pubmed
Coughlin,
c-myc gene expression is stimulated by agents that activate protein kinase C and does not account for the mitogenic effect of PDGF.
1985,
Pubmed
Curran,
Transient induction of c-fos and c-myc in an immediate consequence of growth factor stimulation.
1985,
Pubmed
De Robertis,
Nucleocytoplasmic segregation of proteins and RNAs.
1983,
Pubmed
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Dony,
Post-transcriptional control of myc and p53 expression during differentiation of the embryonal carcinoma cell line F9.
,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Eisenman,
V-myc- and c-myc-encoded proteins are associated with the nuclear matrix.
1985,
Pubmed
Endo,
Transcriptional and posttranscriptional control of c-myc during myogenesis: its mRNA remains inducible in differentiated cells and does not suppress the differentiated phenotype.
1986,
Pubmed
Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase
Fukui,
Role of a ras homolog in the life cycle of Schizosaccharomyces pombe.
1986,
Pubmed
Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase
Gonda,
Expression of myb, myc and fos proto-oncogenes during the differentiation of a murine myeloid leukaemia.
,
Pubmed
Gonda,
Transcripts from the cellular homologs of retroviral oncogenes: distribution among chicken tissues.
1982,
Pubmed
Greenberg,
Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
,
Pubmed
Grosso,
Transcriptional regulation of c-myc during chemically induced differentiation of HL-60 cultures.
1985,
Pubmed
Hann,
Proteins encoded by v-myc and c-myc oncogenes: identification and localization in acute leukemia virus transformants and bursal lymphoma cell lines.
1983,
Pubmed
Hann,
Proteins encoded by the human c-myc oncogene: differential expression in neoplastic cells.
1984,
Pubmed
Hann,
c-myc oncogene protein synthesis is independent of the cell cycle in human and avian cells.
,
Pubmed
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Kelly,
Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.
1983,
Pubmed
Lehrach,
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
1977,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Miake-Lye,
Induction of early mitotic events in a cell-free system.
1985,
Pubmed
,
Xenbase
Newmeyer,
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
1986,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase
Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase
Richter,
The mechanism for increased protein synthesis during Xenopus oocyte maturation.
1982,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Simon,
The nucleotide sequence and the tissue-specific expression of Drosophila c-src.
1985,
Pubmed
Steele,
Two divergent cellular src genes are expressed in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed
Thompson,
Levels of c-myc oncogene mRNA are invariant throughout the cell cycle.
,
Pubmed
Toda,
In yeast, RAS proteins are controlling elements of adenylate cyclase.
1985,
Pubmed
Wasserman,
Protein synthesis during maturation promoting factor- and progesterone-induced maturation in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Watson,
Nucleotide sequence analysis of the chicken c-myc gene reveals homologous and unique coding regions by comparison with the transforming gene of avian myelocytomatosis virus MC29, delta gag-myc.
1983,
Pubmed
Watt,
Nucleotide sequence of cloned cDNA of human c-myc oncogene.
,
Pubmed
Zimmerman,
Differential expression of myc family genes during murine development.
,
Pubmed