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J Cell Biochem
1987 Oct 01;352:129-36. doi: 10.1002/jcb.240350206.
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Expression of coronavirus E1 and rotavirus VP10 membrane proteins from synthetic RNA.
Armstrong J
,
McCrae M
,
Colman A
.
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Some viruses acquire their envelopes by budding through internal membranes of their host cell. We have expressed the cloned cDNA for glycoproteins from two such viruses, the E1 protein of coronavirus, which buds in the Golgi region, and VP10 protein of rotavirus, which assembles in the endoplasmic reticulum. Messenger RNA was prepared from both cDNAs by using SP6 polymerase and either translated in vitro or injected into cultured CV1 cells or Xenopus oocytes. In CV1 cells, the E1 protein was localised to the Golgi region and VP10 protein to the endoplasmic reticulum. In Xenopus oocytes, the E1 protein acquired post-translational modifications indistinguishable from the sialylated, O-linked sugars found on viral protein, while the VP10 protein acquired endoglycosidase-H-sensitive N-linked sugars, consistent with their localisation to the Golgi complex and endoplasmic reticulum, respectively. Thus the two proteins provide models with which to study targeting to each of these intracellular compartments. When the RNAs were expressed in matured, meiotic oocytes, the VP10 protein was modified as before, but the E1 protein was processed to a much lesser extent than in interphase oocytes, consistent with a cessation of vesicular transport during cell division.
Altenburg,
Ultrastructural study of rotavirus replication in cultured cells.
1980, Pubmed
Altenburg,
Ultrastructural study of rotavirus replication in cultured cells.
1980,
Pubmed
Armstrong,
Sequence and topology of a model intracellular membrane protein, E1 glycoprotein, from a coronavirus.
,
Pubmed
Armstrong,
Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
1983,
Pubmed
Baybutt,
The molecular biology of rotaviruses. VII. Detailed structural analysis of gene 10 of bovine rotavirus.
1984,
Pubmed
Bole,
Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas.
1986,
Pubmed
Colman,
Meiotic maturation in Xenopus oocytes: a link between the cessation of protein secretion and the polarized disappearance of Golgi apparati.
1985,
Pubmed
,
Xenbase
DAVID-FERREIRA,
AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF A MOUSE HEPATITIS VIRUS IN TISSUE CULTURE CELLS.
1965,
Pubmed
Drummond,
The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Dubois-Dalcq,
Cell tropism and expression of mouse hepatitis viruses (MHV) in mouse spinal cord cultures.
1982,
Pubmed
Ericson,
Two types of glycoprotein precursors are produced by the simian rotavirus SA11.
1983,
Pubmed
Gething,
Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport.
1986,
Pubmed
Hortsch,
Characterization of secretory protein translocation: ribosome-membrane interaction in endoplasmic reticulum.
1986,
Pubmed
Kabcenell,
Processing of the rough endoplasmic reticulum membrane glycoproteins of rotavirus SA11.
1985,
Pubmed
Kelly,
Pathways of protein secretion in eukaryotes.
1985,
Pubmed
Kornfeld,
Assembly of asparagine-linked oligosaccharides.
1985,
Pubmed
Kreibich,
Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes.
1978,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
McCrae,
Molecular biology of rotaviruses. I. Characterization of basic growth parameters and pattern of macromolecular synthesis.
1981,
Pubmed
Munro,
An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.
1986,
Pubmed
Niemann,
Coronavirus glycoprotein E1, a new type of viral glycoprotein.
1981,
Pubmed
Niemann,
The carbohydrates of mouse hepatitis virus (MHV) A59: structures of the O-glycosidically linked oligosaccharides of glycoprotein E1.
1984,
Pubmed
Niemann,
Post-translational glycosylation of coronavirus glycoprotein E1: inhibition by monensin.
1982,
Pubmed
Palade,
Intracellular aspects of the process of protein synthesis.
1975,
Pubmed
Petrie,
Effects of tunicamycin on rotavirus morphogenesis and infectivity.
1983,
Pubmed
Poruchynsky,
Deletions into an NH2-terminal hydrophobic domain result in secretion of rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein.
1985,
Pubmed
Rottier,
Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase
Rottier,
Assembly in vitro of a spanning membrane protein of the endoplasmic reticulum: the E1 glycoprotein of coronavirus mouse hepatitis virus A59.
1984,
Pubmed
Soler,
Association of viral particles and viral proteins with membranes in SA11-infected cells.
1982,
Pubmed
Tarentino,
Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.
1974,
Pubmed
Tooze,
Replication of coronavirus MHV-A59 in sac- cells: determination of the first site of budding of progeny virions.
1984,
Pubmed
Tooze,
Identification of two epitopes in the carboxyterminal 15 amino acids of the E1 glycoprotein of mouse hepatitis virus A59 by using hybrid proteins.
1986,
Pubmed
