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The secondary structure of the 7SL RNA in the signal recognition particle: functional implications.
Zwieb C
.
Abstract
The secondary structure of the 7SL RNA in the signal recognition particle was determined by applying both a theoretical and an experimental approach. The compensatory base change approach was taken comparing the published sequences of human, Drosophila and Xenopus 7SL RNA's. The deduced secondary structure was confirmed by post-labeling of an RNase V1-nicked dog SRP with P32-pCp and RNA-ligase and analysis of the labeled RNA-fragments by non-denaturing/denaturing 2D polyacrylamide gel electrophoresis. Two interesting features in the secondary structure were revealed: Firstly, bases at positions 122 to 127 of the human 7SL RNA are not only able to pair with bases at positions 167 to 170, but also with a single-stranded region of the bases at positions 223 to 228, suggesting an alternative base pairing scheme for the 7SL RNA in all three organisms. In agreement with this finding, four different conformations were identified after transcription of the 7SL RNA from the genomic human clone. The involvement of the particular basepairing interaction postulated was confirmed by the analysis of a 7SL RNA deletion mutant (Sma1-409). Secondly, a significant sequence homology of the paired bases at positions 236 to 255 and 104 to 109 in 7SL RNA with bases in 5S ribosomal RNA at the positions 84 to 110 was noticed, suggesting that 5S ribosomal and 7SL RNA interact with the same target during protein biosynthesis. These findings are summarized by proposing a mechanism for the translational arrest of protein synthesis by the signal recognition particle using specific sequences and an alternative configuration in the 7SL RNA.
Andrews,
Structure of the signal recognition particle by electron microscopy.
1985, Pubmed
Andrews,
Structure of the signal recognition particle by electron microscopy.
1985,
Pubmed
Ansorge,
System for DNA sequencing with resolution of up to 600 base pairs.
1984,
Pubmed
Brimacombe,
The structure of ribosomal RNA and its organization relative to ribosomal protein.
1983,
Pubmed
Cech,
Secondary structure of the Tetrahymena ribosomal RNA intervening sequence: structural homology with fungal mitochondrial intervening sequences.
1983,
Pubmed
Clark,
Xenopus laevis 28S ribosomal RNA: a secondary structure model and its evolutionary and functional implications.
1984,
Pubmed
,
Xenbase
Dagert,
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
1979,
Pubmed
Dahlberg,
Specific binding of tRNAMet to 23S rRNA of Escherichia coli.
1978,
Pubmed
Dingman,
Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis.
1968,
Pubmed
Donis-Keller,
Mapping adenines, guanines, and pyrimidines in RNA.
1977,
Pubmed
Erdmann,
Collection of published 5S and 5.8S ribosomal RNA sequences.
1984,
Pubmed
Fox,
5S RNA secondary structure.
1975,
Pubmed
,
Xenbase
Gilmore,
Protein translocation across the endoplasmic reticulum. I. Detection in the microsomal membrane of a receptor for the signal recognition particle.
1982,
Pubmed
Glotz,
Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes.
1981,
Pubmed
Guerrier-Takada,
Catalytic activity of an RNA molecule prepared by transcription in vitro.
1984,
Pubmed
Gundelfinger,
The organization of the 7SL RNA in the signal recognition particle.
1983,
Pubmed
Gundelfinger,
Structure and evolution of the 7SL RNA component of the signal recognition particle.
1984,
Pubmed
Jelinek,
Repetitive sequences in eukaryotic DNA and their expression.
1982,
Pubmed
Maizel,
Enhanced graphic matrix analysis of nucleic acid and protein sequences.
1981,
Pubmed
Meyer,
Secretory protein translocation across membranes-the role of the "docking protein'.
1982,
Pubmed
Meyer,
A membrane component essential for vectorial translocation of nascent proteins across the endoplasmic reticulum: requirements for its extraction and reassociation with the membrane.
1980,
Pubmed
Noller,
Topography of 16S RNA in 30S ribosomal subunits. Nucleotide sequences and location of sites of reaction with kethoxal.
1974,
Pubmed
Ross,
Experimental determination of interacting sequences in ribosomal RNA.
1979,
Pubmed
Ullu,
Human genes and pseudogenes for the 7SL RNA component of signal recognition particle.
1984,
Pubmed
Ullu,
Alu sequences are processed 7SL RNA genes.
,
Pubmed
,
Xenbase
Ullu,
Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an alu sequence.
1982,
Pubmed
Vassilenko,
Topography of 16 S RNA in 30 S subunits and 70 S ribosomes accessibility to cobra venom ribonuclease.
1981,
Pubmed
Volckaert,
Micro thin-layer techniques for rapid sequence analysis of 32P-labeled RNA: double digestion and pancreatic ribonuclease analyses.
1977,
Pubmed
Walter,
Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum.
1982,
Pubmed
Walter,
Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.
1981,
Pubmed
Walter,
Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein.
1981,
Pubmed
Walter,
Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.
1980,
Pubmed
Walter,
Protein translocation across the endoplasmic reticulum.
1984,
Pubmed
Warren,
Protein transfer across microsomal membranes reassembled from separated membrane components.
1978,
Pubmed
Zieve,
Small RNA species of the HeLa cell: metabolism and subcellular localization.
1976,
Pubmed
Zweib,
Structural and functional analysis of Escherichia coli ribosomes containing small deletions around position 1760 in the 23S ribosomal RNA.
1984,
Pubmed
