Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Alternative splicing of SV40 early pre-mRNA in vitro.
van Santen VL
,
Spritz RA
.
Abstract
Simian virus 40 (SV40) early pre-mRNA is spliced using either of two alternative 5' splice sites and a common 3' splice site to produce two mRNAs that encode the T and t antigens. We have studied alternative splicing of SV40 early pre-mRNA in vitro using a HeLa cell nuclear extract. Synthetic SV40 early transcripts are processed to T and t antigen mRNAs in vitro. As in SV40-infected cells in vivo, cleavage at the T antigen 5' splice site is more efficient than cleavage at the t antigen 5' splice site in vitro, although both of these 5' splice sites are utilized relatively inefficiently in vitro. The ratio of cleavage at the T and t antigen 5' splice sites is not changed significantly by a number of alterations in the conditions under which the in vitro splicing reactions are carried out.
Alwine,
Control of simian virus 40 gene expression at the levels of RNA synthesis and processing: thermally induced changes in the ratio of the simian virus 40 early mRNA's and proteins.
1980, Pubmed
Alwine,
Control of simian virus 40 gene expression at the levels of RNA synthesis and processing: thermally induced changes in the ratio of the simian virus 40 early mRNA's and proteins.
1980,
Pubmed
Berger,
Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes.
1979,
Pubmed
Black,
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.
1985,
Pubmed
Deutscher,
Novel deletion mutants that enhance a distant upstream 5' splice in the E3 transcription unit of adenovirus 2.
1985,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Fradin,
Splicing pathways of SV40 mRNAs in X. laevis oocytes differ in their requirements for snRNPs.
1984,
Pubmed
,
Xenbase
Fromm,
Deletion mapping of DNA regions required for SV40 early region promoter function in vivo.
1982,
Pubmed
Ghosh,
Unspliced functional late 19S mRNAs containing intervening sequences are produced by a late leader mutant of simian virus 40.
1981,
Pubmed
Ghosh,
Simian virus 40 early mRNA's contain multiple 5' termini upstream and downstream from a Hogness-Goldberg sequence; a shift in 5' termini during the lytic cycle is mediated by large T antigen.
1981,
Pubmed
Gluzman,
SV40-transformed simian cells support the replication of early SV40 mutants.
1981,
Pubmed
Hansen,
T antigen repression of SV40 early transcription from two promoters.
1981,
Pubmed
Hardy,
Cofactor requirements of splicing of purified messenger RNA precursors.
,
Pubmed
Hernandez,
Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts.
1983,
Pubmed
Khoury,
New chimeric splice junction in adenovirus type 2-simian virus 40 hybrid viral mRNA.
1980,
Pubmed
Khoury,
Processing and expression of early SV40 mRNA: a role for RNA conformation in splicing.
1979,
Pubmed
Krainer,
Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.
1984,
Pubmed
Lang,
The two intervening sequences of human beta- and gamma-globin pre-mRNAs are excised in a preferred temporal order in vitro.
1985,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Mohr,
Application of ribonuclease T1 to the synthesis of oligoribonucleotides of defined base sequence.
1969,
Pubmed
Noble,
In vitro splicing of simian virus 40 early pre mRNA.
1986,
Pubmed
Padgett,
Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.
1984,
Pubmed
Padgett,
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
1983,
Pubmed
Reddy,
Simian virus 40 early mRNA's. I. Genomic localization of 3' and 5' termini and two major splices in mRNA from transformed and lytically infected cells.
1979,
Pubmed
Ross,
A precursor of globin messenger RNA.
1976,
Pubmed
Ruskin,
Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.
1984,
Pubmed
Solnick,
Alternative splicing caused by RNA secondary structure.
1985,
Pubmed
Somasekhar,
Exon mutations that affect the choice of splice sites used in processing the SV40 late transcripts.
1985,
Pubmed
White,
Simian virus 40-rabbit beta-globin recombinants lacking late mRNA splice sites express cytoplasmic RNAs with altered structures.
1982,
Pubmed
Wickens,
Role of the conserved AAUAAA sequence: four AAUAAA point mutants prevent messenger RNA 3' end formation.
1984,
Pubmed
Yang,
A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.
1981,
Pubmed
