Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1997 May 13;9410:5432-7. doi: 10.1073/pnas.94.10.5432.
Show Gene links
Show Anatomy links
Peptidyl prolyl cis-trans isomerase activity of cyclophilin A in functional homo-oligomeric receptor expression.
Helekar SA
,
Patrick J
.
Abstract
The functional expression of homo-oligomeric alpha7 neuronal nicotinic and type 3 serotonin receptors is dependent on the activity of a cyclophilin. In this paper we demonstrate that the mechanism of cyclophilin action during functional homo-oligomeric receptor expression in Xenopus oocytes is distinct from the calcineurin-dependent immunosuppressive mechanism by showing that a nonimmunosuppressive analog of cyclosporin A (CsA), SDZ 211-811, reduces functional receptor expression to the same extent as CsA. The cytoplasmic subtype of cyclophilin, cyclophilin A (CyPA), appears to be required for functional receptor expression. This is because overexpression of CyPA and a CyPA mutant that is deficient in CsA binding activity reverses CsA-induced reduction in functional receptor expression. The mechanism of action of CyPA is likely to involve its prolyl isomerase activity because a mutant CyPA with a single amino acid substitution (arginine 55 to alanine) that is predicted to produce a 1000-fold attenuation in isomerase activity fails to reverse the cyclosporin A effect. Our data also suggest that CyPA does not form a stable complex with receptor subunits.
Baker,
The cyclophilin homolog NinaA functions as a chaperone, forming a stable complex in vivo with its protein target rhodopsin.
1994, Pubmed
Baker,
The cyclophilin homolog NinaA functions as a chaperone, forming a stable complex in vivo with its protein target rhodopsin.
1994,
Pubmed
Billich,
Mode of action of SDZ NIM 811, a nonimmunosuppressive cyclosporin A analog with activity against human immunodeficiency virus (HIV) type 1: interference with HIV protein-cyclophilin A interactions.
1995,
Pubmed
Brillantes,
Stabilization of calcium release channel (ryanodine receptor) function by FK506-binding protein.
1994,
Pubmed
Cameron,
Immunophilin FK506 binding protein associated with inositol 1,4,5-trisphosphate receptor modulates calcium flux.
1995,
Pubmed
Fischer,
The mechanism of protein folding. Implications of in vitro refolding models for de novo protein folding and translocation in the cell.
1990,
Pubmed
Fischer,
[Determination of enzymatic catalysis for the cis-trans-isomerization of peptide binding in proline-containing peptides].
1984,
Pubmed
Franke,
Specific incorporation of cyclophilin A into HIV-1 virions.
1994,
Pubmed
Freskgård,
Isomerase and chaperone activity of prolyl isomerase in the folding of carbonic anhydrase.
1992,
Pubmed
Fruman,
Immunophilins in protein folding and immunosuppression.
1994,
Pubmed
Gething,
Protein folding in the cell.
1992,
Pubmed
Harding,
A receptor for the immunosuppressant FK506 is a cis-trans peptidyl-prolyl isomerase.
1989,
Pubmed
Helekar,
Prolyl isomerase requirement for the expression of functional homo-oligomeric ligand-gated ion channels.
1994,
Pubmed
,
Xenbase
Horton,
Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.
1990,
Pubmed
Iwai,
Molecular cloning of a complementary DNA to rat cyclophilin-like protein mRNA.
1990,
Pubmed
Johnson,
A novel chaperone complex for steroid receptors involving heat shock proteins, immunophilins, and p23.
1994,
Pubmed
Kern,
Reassessment of the putative chaperone function of prolyl-cis/trans-isomerases.
1994,
Pubmed
Kern,
A kinetic analysis of the folding of human carbonic anhydrase II and its catalysis by cyclophilin.
1995,
Pubmed
Kofron,
Determination of kinetic constants for peptidyl prolyl cis-trans isomerases by an improved spectrophotometric assay.
1991,
Pubmed
Lang,
Catalysis of protein folding by prolyl isomerase.
,
Pubmed
Lang,
Protein-disulphide isomerase and prolyl isomerase act differently and independently as catalysts of protein folding.
1988,
Pubmed
Lin,
Catalysis of proline isomerization during protein-folding reactions.
1988,
Pubmed
Liu,
Human and Escherichia coli cyclophilins: sensitivity to inhibition by the immunosuppressant cyclosporin A correlates with a specific tryptophan residue.
1991,
Pubmed
Liu,
Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes.
1991,
Pubmed
Lodish,
Cyclosporin A inhibits an initial step in folding of transferrin within the endoplasmic reticulum.
1991,
Pubmed
Matouschek,
Cyclophilin catalyzes protein folding in yeast mitochondria.
1995,
Pubmed
Price,
Human cyclophilin B: a second cyclophilin gene encodes a peptidyl-prolyl isomerase with a signal sequence.
1991,
Pubmed
Schmid,
Prolyl isomerases: role in protein folding.
1993,
Pubmed
Schneuwly,
Drosophila ninaA gene encodes an eye-specific cyclophilin (cyclosporine A binding protein).
1989,
Pubmed
Schreiber,
Immunophilin-sensitive protein phosphatase action in cell signaling pathways.
1992,
Pubmed
Shieh,
The ninaA gene required for visual transduction in Drosophila encodes a homologue of cyclosporin A-binding protein.
1989,
Pubmed
Steinmann,
Cyclosporin A slows collagen triple-helix formation in vivo: indirect evidence for a physiologic role of peptidyl-prolyl cis-trans-isomerase.
1991,
Pubmed
Thali,
Functional association of cyclophilin A with HIV-1 virions.
1994,
Pubmed
Timerman,
The calcium release channel of sarcoplasmic reticulum is modulated by FK-506-binding protein. Dissociation and reconstitution of FKBP-12 to the calcium release channel of skeletal muscle sarcoplasmic reticulum.
1993,
Pubmed
Zydowsky,
Active site mutants of human cyclophilin A separate peptidyl-prolyl isomerase activity from cyclosporin A binding and calcineurin inhibition.
1992,
Pubmed
