Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The hemispheric functional expression of the thyrotropin-releasing-hormone receptor is not determined by the receptors' physical distribution.
Matus-Leibovitch N
,
Nussenzveig DR
,
Gershengorn MC
,
Oron Y
.
???displayArticle.abstract???
The thyrotropin-releasing-hormone receptor (TRH-R) is a member of a family of the G-protein-coupled receptors that share structural similarities and exert their physiological action via the inositol lipid signal-transduction pathway. The TRH-R when expressed in Xenopus oocytes exhibits marked preference of the response (increased chloride conductance) for the animal hemisphere. Whereas the rat TRH-R functional distribution was strongly asymmetric (animal/vegetal ratio = 9.5), the mouse TRH-R exhibited a significantly lower ratio (3.9). Truncation of the last 59 amino acids of the C-terminal region of the mouse TRH-R did not lead to any changes in the functional hemispheric distribution. Despite the polarization of response, receptor number was similar on both hemispheres. Moreover, the apparent half-life of the functional expression of the TRH-R was approx. 4 h on both hemispheres when the expression was inhibited by a specific antisense oligonucleotide. Inhibition of total protein synthesis with cycloheximide affected hemispheric responses mediated by each of the three TRH-Rs tested in a qualitatively different way. These results suggest that an additional, rapidly degraded, protein modulates the functional hemispheric expression of the TRH-Rs.
Bartles,
Plasma membrane protein sorting in epithelial cells: do secretory pathways hold the key?
1988, Pubmed
Bartles,
Plasma membrane protein sorting in epithelial cells: do secretory pathways hold the key?
1988,
Pubmed
Buck,
Characterization of N- and C-terminal deletion mutants of the rat serotonin HT2 receptor in Xenopus laevis oocytes.
1991,
Pubmed
,
Xenbase
Casanova,
Phosphorylation of the polymeric immunoglobulin receptor required for its efficient transcytosis.
1990,
Pubmed
Cavalieri,
Induction and decay of human fibroblast interferon mRNA.
1977,
Pubmed
,
Xenbase
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
de la Peña,
Cloning and expression of the thyrotropin-releasing hormone receptor from GH3 rat anterior pituitary cells.
1992,
Pubmed
,
Xenbase
Farquhar,
Intracellular membrane traffic: pathways, carriers, and sorting devices.
1983,
Pubmed
Kobilka,
Chimeric alpha 2-,beta 2-adrenergic receptors: delineation of domains involved in effector coupling and ligand binding specificity.
1988,
Pubmed
Lechleiter,
Subcellular patterns of calcium release determined by G protein-specific residues of muscarinic receptors.
1991,
Pubmed
,
Xenbase
Lipinsky,
Latency in the inositol lipid transduction pathway: the role of cellular events in responses to thyrotropin-releasing hormone in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Lipinsky,
G alpha 11 and G alpha q guanine nucleotide regulatory proteins differentially modulate the response to thyrotropin-releasing hormone in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Lupu-Meiri,
Hemispheric asymmetry of rapid chloride responses to inositol trisphosphate and calcium in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Lupu-Meiri,
Two types of intrinsic muscarinic responses in Xenopus oocytes. I. Differences in latencies and 45Ca efflux kinetics.
1990,
Pubmed
,
Xenbase
Lupu-Meiri,
Dual regulation by protein kinase C of the muscarinic response in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Matlin,
The sorting of proteins to the plasma membrane in epithelial cells.
1986,
Pubmed
Matus-Leibovitch,
Two types of intrinsic muscarinic responses in Xenopus oocytes. II. Hemispheric asymmetry of responses and receptor distribution.
1990,
Pubmed
,
Xenbase
Matus-Leibovitch,
Kinetics of the functional loss of different muscarinic receptor isoforms in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Matus-Leibovitch,
Differential effects of cytoskeletal agents on hemispheric functional expression of cell membrane receptors in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Nussenzveig,
Agonist-stimulated internalization of the thyrotropin-releasing hormone receptor is dependent on two domains in the receptor carboxyl terminus.
1993,
Pubmed
Oron,
The hemispheric distribution of Torpedo nicotinic receptors expressed in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Oron,
Mechanism of membrane electrical response to thyrotropin-releasing hormone in Xenopus oocytes injected with GH3 pituitary cell messenger ribonucleic acid.
1987,
Pubmed
,
Xenbase
Oron,
Differences in receptor-evoked membrane electrical responses in native and mRNA-injected Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Peter,
The polarized distribution of poly(A+)-mRNA-induced functional ion channels in the Xenopus oocyte plasma membrane is prevented by anticytoskeletal drugs.
1991,
Pubmed
,
Xenbase
Richter,
Adenovirus E1A requires synthesis of a cellular protein to establish a stable transcription complex in injected Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase
Robinson,
Electrical currents through full-grown and maturing Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Rodriguez Boulan,
Asymmetric budding of viruses in epithelial monlayers: a model system for study of epithelial polarity.
1978,
Pubmed
Simons,
Polarized sorting in epithelia.
1990,
Pubmed
Simons,
Cell surface polarity in epithelia.
1985,
Pubmed
Straub,
Receptor number determines latency and amplitude of the thyrotropin-releasing hormone response in Xenopus oocytes injected with pituitary RNA.
1989,
Pubmed
,
Xenbase
Straub,
Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor.
1990,
Pubmed
,
Xenbase
Wollner,
Establishing and maintaining epithelial cell polarity. Roles of protein sorting, delivery and retention.
1992,
Pubmed
Zhao,
Molecular cloning of a complementary deoxyribonucleic acid encoding the thyrotropin-releasing hormone receptor and regulation of its messenger ribonucleic acid in rat GH cells.
1992,
Pubmed
,
Xenbase
