XB-ART-14412
Methods Enzymol
1998 Jan 01;293:504-29. doi: 10.1016/s0076-6879(98)93031-2.
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In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system.
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A general method for the incorporation of unnatural amino acids into ion channels and membrane receptors using a Xenopus oocyte expression system has been described. A large number of unnatural amino acids have been incorporated into the nAChR, GIRK, and Shaker K+ channels. Continuing efforts focus on incorporating unnatural amino acids that differ substantially from the natural amino acids, for example, residues that include fluorophores. In addition, we are addressing the feasibility of incorporating unnatural amino acids into ion channels and membrane receptors in mammalian cells.
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Species referenced: Xenopus laevis
Genes referenced: kcnj3