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Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes.
Gorr IH
,
Reis A
,
Boos D
,
Wühr M
,
Madgwick S
,
Jones KT
,
Stemmann O
.
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Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.
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