Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The Fanconi anemia protein FANCM is controlled by FANCD2 and the ATR/ATM pathways.
Sobeck A
,
Stone S
,
Landais I
,
de Graaf B
,
Hoatlin ME
.
???displayArticle.abstract???
Genomic stability requires a functional Fanconi anemia (FA) pathway composed of an upstream "core complex" (FA proteins A/B/C/E/F/G/L/M) that mediates monoubiquitination of the downstream targets FANCD2 and FANCI. Unique among FA core complex members, FANCM has processing activities toward replication-associated DNA structures, suggesting a vital role for FANCM during replication. Using Xenopus egg extracts, we analyzed the functions of FANCM in replication and the DNA damage response. xFANCM binds chromatin in a replication-dependent manner and is phosphorylated in response to DNA damage structures. Chromatin binding and DNA damage-induced phosphorylation of xFANCM are mediated in part by the downstream FA pathway protein FANCD2. Moreover, phosphorylation and chromatin recruitment of FANCM is regulated by two mayor players in the DNA damage response: the cell cycle checkpoint kinases ATR and ATM. Our results indicate that functions of FANCM are controlled by FA- and non-FA pathways in the DNA damage response.
Andreassen,
ATR couples FANCD2 monoubiquitination to the DNA-damage response.
2004, Pubmed
Andreassen,
ATR couples FANCD2 monoubiquitination to the DNA-damage response.
2004,
Pubmed
Ball,
ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation.
2005,
Pubmed
Ball,
ATRIP oligomerization is required for ATR-dependent checkpoint signaling.
2005,
Pubmed
Bomgarden,
A novel protein activity mediates DNA binding of an ATR-ATRIP complex.
2004,
Pubmed
Centurion,
Damage-resistant DNA synthesis in Fanconi anemia cells treated with a DNA cross-linking agent.
2000,
Pubmed
Cimprich,
Probing ATR activation with model DNA templates.
2007,
Pubmed
,
Xenbase
Collis,
FANCM and FAAP24 function in ATR-mediated checkpoint signaling independently of the Fanconi anemia core complex.
2008,
Pubmed
Cortez,
ATR and ATRIP: partners in checkpoint signaling.
2001,
Pubmed
Costanzo,
Mre11 protein complex prevents double-strand break accumulation during chromosomal DNA replication.
2001,
Pubmed
,
Xenbase
Costanzo,
Mre11 assembles linear DNA fragments into DNA damage signaling complexes.
2004,
Pubmed
,
Xenbase
D'Andrea,
The Fanconi anaemia/BRCA pathway.
2003,
Pubmed
Freie,
A role for the Fanconi anemia C protein in maintaining the DNA damage-induced G2 checkpoint.
2004,
Pubmed
Garcia-Higuera,
Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway.
2001,
Pubmed
Gari,
The Fanconi anemia protein FANCM can promote branch migration of Holliday junctions and replication forks.
2008,
Pubmed
Gregory,
Regulation of the Fanconi anemia pathway by monoubiquitination.
2003,
Pubmed
Guervilly,
Loss of CHK1 function impedes DNA damage-induced FANCD2 monoubiquitination but normalizes the abnormal G2 arrest in Fanconi anemia.
2008,
Pubmed
Hickson,
Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM.
2004,
Pubmed
Ho,
Phosphorylation of FANCD2 on two novel sites is required for mitomycin C resistance.
2006,
Pubmed
Kee,
Regulated degradation of FANCM in the Fanconi anemia pathway during mitosis.
2009,
Pubmed
Kim,
Phosphorylation of Chk1 by ATM- and Rad3-related (ATR) in Xenopus egg extracts requires binding of ATRIP to ATR but not the stable DNA-binding or coiled-coil domains of ATRIP.
2005,
Pubmed
,
Xenbase
Kim,
Cell cycle-dependent chromatin loading of the Fanconi anemia core complex by FANCM/FAAP24.
2008,
Pubmed
Lavin,
ATM and the Mre11 complex combine to recognize and signal DNA double-strand breaks.
2007,
Pubmed
Lee,
Activation and regulation of ATM kinase activity in response to DNA double-strand breaks.
2007,
Pubmed
Lupardus,
A requirement for replication in activation of the ATR-dependent DNA damage checkpoint.
2002,
Pubmed
,
Xenbase
Lupardus,
Phosphorylation of Xenopus Rad1 and Hus1 defines a readout for ATR activation that is independent of Claspin and the Rad9 carboxy terminus.
2006,
Pubmed
,
Xenbase
Lupardus,
Analyzing the ATR-mediated checkpoint using Xenopus egg extracts.
2007,
Pubmed
,
Xenbase
MacDougall,
The structural determinants of checkpoint activation.
2007,
Pubmed
,
Xenbase
Matsuoka,
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
2007,
Pubmed
Meetei,
A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M.
2005,
Pubmed
Mosedale,
The vertebrate Hef ortholog is a component of the Fanconi anemia tumor-suppressor pathway.
2005,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Pichierri,
The DNA crosslink-induced S-phase checkpoint depends on ATR-CHK1 and ATR-NBS1-FANCD2 pathways.
2004,
Pubmed
Rosselli,
The Fanconi anemia pathway and the DNA interstrand cross-links repair.
2003,
Pubmed
Sala-Trepat,
Arrest of S-phase progression is impaired in Fanconi anemia cells.
2000,
Pubmed
Shechter,
ATR and ATM regulate the timing of DNA replication origin firing.
2004,
Pubmed
,
Xenbase
Shechter,
ATM and ATR check in on origins: a dynamic model for origin selection and activation.
2005,
Pubmed
,
Xenbase
Smogorzewska,
Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair.
2007,
Pubmed
Sobeck,
Fanconi anemia proteins are required to prevent accumulation of replication-associated DNA double-strand breaks.
2006,
Pubmed
,
Xenbase
Sobeck,
DNA structure-induced recruitment and activation of the Fanconi anemia pathway protein FANCD2.
2007,
Pubmed
,
Xenbase
Taniguchi,
Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways.
2002,
Pubmed
Taniguchi,
S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51.
2002,
Pubmed
Wang,
Chk1-mediated phosphorylation of FANCE is required for the Fanconi anemia/BRCA pathway.
2007,
Pubmed
Wang,
Emergence of a DNA-damage response network consisting of Fanconi anaemia and BRCA proteins.
2007,
Pubmed
Xue,
FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair.
2008,
Pubmed
Yoo,
Site-specific phosphorylation of a checkpoint mediator protein controls its responses to different DNA structures.
2006,
Pubmed
,
Xenbase
Zhu,
An ATR- and BRCA1-mediated Fanconi anemia pathway is required for activating the G2/M checkpoint and DNA damage repair upon rereplication.
2006,
Pubmed
Zou,
Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes.
2003,
Pubmed