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Methods Mol Biol
2011 Jan 01;714:71-82. doi: 10.1007/978-1-61779-005-8_5.
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Visualization of mRNA localization in Xenopus oocytes.
Gagnon JA
,
Mowry KL
.
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Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution.
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