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J Membr Biol
1995 May 01;1452:193-204. doi: 10.1007/bf00237377.
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Cytoskeletal disruption in A6 kidney cells: impact on endo/exocytosis and NaCl transport regulation by antidiuretic hormone.
Verrey F
,
Groscurth P
,
Bolliger U
.
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Antidiuretic hormone (ADH; 2.5 x 10(-8) M vasotocin) produces a stimulation of apical fluid phase endocytosis, protein secretion and NaCl reabsorption in Xenopus laevis A6 distalnephron cell epithelia pretreated with aldosterone (10(-6) M). The increase of NaCl transport is mediated by a sequential opening of apical Cl and Na conductances. The aim of this study was to characterize the actin and tubulin cytoskeleton of A6 cells and to assess the impact of its disruption on baseline and ADH-induced apical vesicular membrane movements and ion transport to test for possible functional links. The microfilament (MF) and microtubule (MT) networks and their disruption were visualized by confocal laser microscopy. Conditions of depolimerization were selected, by cytochalasin D or cold and nocodazole, respectively. MF disruption produced an increase in baseline apical protein secretion (exocytic movements) (plus 18%) and a decrease of its induction by ADH (minus 35%). MF disruption also increased baseline horseradish peroxidase uptake (endocytic movements) (plus 21%), however, without affecting its ADH-induced increase. In the case of MT disruption, the ADH-induced stimulation of both protein secretion and fluid phase endocytosis was decreased by 70 and 44%, respectively. At the ion transport level, MF and MT disruption only insignificantly affected the ADH-induced Cl conductance, while they decreased the ADH-induced stimulation of Na transport (amiloride-sensitive short-circuit current and conductance) by a factor of 2 to 4. In conclusion, both MT and MF disruption decrease ADH-induced apical protein secretion and Na conductance, while the ADH-induced apical Cl conductance is not significantly affected. Taken together the data support the hypothesis that the modulation of Na channel expression by apical vesicular membrane movements plays a role in Na transport expression and its regulation by ADH.
Beron,
Aldosterone induces early activation and late accumulation of Na-K-ATPase at surface of A6 cells.
1994, Pubmed,
Xenbase
Beron,
Aldosterone induces early activation and late accumulation of Na-K-ATPase at surface of A6 cells.
1994,
Pubmed
,
Xenbase
Boll,
Distinct pathways for basolateral targeting of membrane and secretory proteins in polarized epithelial cells.
1991,
Pubmed
Breitfeld,
Effect of nocodazole on vesicular traffic to the apical and basolateral surfaces of polarized MDCK cells.
1990,
Pubmed
Brown,
Endosomal pathways for water channel and proton pump recycling in kidney epithelial cells.
1993,
Pubmed
Canessa,
Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits.
1994,
Pubmed
,
Xenbase
Cantiello,
Actin-binding protein contributes to cell volume regulatory ion channel activation in melanoma cells.
1993,
Pubmed
Chalfant,
Distinct regulation of Na+ reabsorption and Cl- secretion by arginine vasopressin in the amphibian cell line A6.
1993,
Pubmed
,
Xenbase
De Almeida,
Disruption of microtubules alters polarity of basement membrane proteoglycan secretion in epithelial cells.
1991,
Pubmed
Driscoll,
Molecular genetics of cell death in the nematode Caenorhabditis elegans.
1992,
Pubmed
Duchatelle,
Regulation of renal epithelial sodium channels.
1992,
Pubmed
Eilers,
Nocodazole, a microtubule-active drug, interferes with apical protein delivery in cultured intestinal epithelial cells (Caco-2).
1989,
Pubmed
Els,
Activation of epithelial Na channels by hormonal and autoregulatory mechanisms of action.
1991,
Pubmed
Els,
Sodium-dependent regulation of epithelial sodium channel densities in frog skin; a role for the cytoskeleton.
1993,
Pubmed
Fath,
The cytoskeleton in development of epithelial cell polarity.
1993,
Pubmed
Franki,
Effect of cytochalasin D on the actin cytoskeleton of the toad bladder epithelial cell.
1992,
Pubmed
Fuller,
Forskolin- but not ionomycin-evoked Cl- secretion in colonic epithelia depends on intact microtubules.
1994,
Pubmed
Garty,
Molecular properties of epithelial, amiloride-blockable Na+ channels.
1994,
Pubmed
,
Xenbase
Garty,
Characteristics and regulatory mechanisms of the amiloride-blockable Na+ channel.
1988,
Pubmed
Gilbert,
Microtubular organization and its involvement in the biogenetic pathways of plasma membrane proteins in Caco-2 intestinal epithelial cells.
1991,
Pubmed
Gottlieb,
Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells.
1993,
Pubmed
Hansch,
Role of microtubules in the adaptive response to low phosphate of Na/Pi cotransport in opossum kidney cells.
1993,
Pubmed
Kemendy,
Aldosterone alters the open probability of amiloride-blockable sodium channels in A6 epithelia.
1992,
Pubmed
Kleyman,
Arginine vasopressin and forskolin regulate apical cell surface expression of epithelial Na+ channels in A6 cells.
1994,
Pubmed
,
Xenbase
Lieuvin,
Intrinsic microtubule stability in interphase cells.
1994,
Pubmed
Marunaka,
Effects of vasopressin on single Cl- channels in the apical membrane of distal nephron cells (A6).
1993,
Pubmed
Marunaka,
Chloride channels in the apical membrane of a distal nephron A6 cell line.
1990,
Pubmed
Marunaka,
Effects of vasopressin and cAMP on single amiloride-blockable Na channels.
1991,
Pubmed
Masson,
Identification and molecular characterization of E-MAP-115, a novel microtubule-associated protein predominantly expressed in epithelial cells.
1993,
Pubmed
Matthews,
Na-K-2Cl cotransport in intestinal epithelial cells. Influence of chloride efflux and F-actin on regulation of cotransporter activity and bumetanide binding.
1994,
Pubmed
Oh,
Regulation by phosphorylation of purified epithelial Na+ channels in planar lipid bilayers.
1993,
Pubmed
Ojakian,
Antimicrotubule drugs inhibit the polarized insertion of an intracellular glycoprotein pool into the apical membrane of Madin-Darby canine kidney (MDCK) cells.
1992,
Pubmed
Paccolat,
Aldosterone regulation of Na+ transport and Na+-K+-ATPase in A6 cells: role of growth conditions.
1987,
Pubmed
Palmer,
Epithelial Na channels: function and diversity.
1992,
Pubmed
Parczyk,
Microtubules are involved in the secretion of proteins at the apical cell surface of the polarized epithelial cell, Madin-Darby canine kidney.
1989,
Pubmed
Perkins,
Transport properties of toad kidney epithelia in culture.
1981,
Pubmed
,
Xenbase
Prat,
Activation of epithelial Na+ channels by protein kinase A requires actin filaments.
1993,
Pubmed
Rossier,
Epithelial sodium channels.
1994,
Pubmed
Rothe,
Measurement of epithelial electrical characteristics with an automatic voltage clamp device with compensation for solution resistance.
1969,
Pubmed
Schafer,
Regulation of Na+ channels in the cortical collecting duct by AVP and mineralocorticoids.
1992,
Pubmed
Schwiebert,
Actin-based cytoskeleton regulates a chloride channel and cell volume in a renal cortical collecting duct cell line.
1994,
Pubmed
Tousson,
Immunochemical localization of amiloride-sensitive sodium channels in sodium-transporting epithelia.
1989,
Pubmed
Verrey,
Regulation by aldosterone of Na+,K+-ATPase mRNAs, protein synthesis, and sodium transport in cultured kidney cells.
1987,
Pubmed
,
Xenbase
Verrey,
Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.
1994,
Pubmed
,
Xenbase
Verrey,
Polarized membrane movements in A6 kidney cells are regulated by aldosterone and vasopressin/vasotocin.
1993,
Pubmed
,
Xenbase
Watson,
Serum regulates Na+/H+ exchange in Caco-2 cells by a mechanism which is dependent on F-actin.
1992,
Pubmed
Yanase,
Adenosine 3',5'-cyclic monophosphate stimulates chloride secretion in A6 epithelia.
1986,
Pubmed
,
Xenbase
