Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Accumulation of the isolated carboxy-terminal domain of histone H1 in the Xenopus oocytenucleus.
Dingwall C
,
Allan J
.
Abstract
Histone H1 accumulates in the nucleus after injection into the cytoplasm of Xenopus oocytes. A proteolytic fragment of 89 amino acids encompassing the carboxy-terminal domain also accumulates in the nucleus. Lysine, alanine and proline compose 84% of this domain. Accumulation is not due solely to the high lysine content since poly-L-lysine does not accumulate in the nucleus when injected into the cytoplasm of Xenopus oocytes. Proteolytic fragments encompassing other domains of the molecule are degraded in the oocyte after injection. In these instances degradation is more rapid in the cytoplasm than in the nucleus giving the false impression of accumulation in the nucleus, an artefact which is likely to confuse other studies of protein migration. Susceptibility to rapid degradation is a dominant feature, thus the globular domain destabilises the contiguous carboxy-terminal domain. The properties of the carboxy-terminal domain of H1 and the possible involvement of the amino acids lysine, proline and alanine in migration are discussed and compared with those of a domain that specifies migration of nucleoplasmin into the oocytenucleus.
Bigelow,
The selective degradation of injected proteins occurs principally in the cytosol rather than in lysosomes.
1981, Pubmed
Bigelow,
The selective degradation of injected proteins occurs principally in the cytosol rather than in lysosomes.
1981,
Pubmed
Bolton,
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.
1973,
Pubmed
Bonner,
Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.
1975,
Pubmed
,
Xenbase
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase
Böhm,
Precise elimination of the N-terminal domain of histone H1.
1982,
Pubmed
Capecchi,
Selective degradation of abnormal proteins in mammalian tissue culture cells.
1974,
Pubmed
Chapman,
Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The isolation of the globular and non-globular regions of the histone H1 molecule.
1976,
Pubmed
Colman,
Fate of secretory proteins trapped in oocytes of Xenopus laevis by disruption of the cytoskeleton or by imbalanced subunit synthesis.
1981,
Pubmed
,
Xenbase
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase
Gurdon,
Nuclear transplantation and the control of gene activity in animal development.
1970,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Hall,
Targeting of E. coli beta-galactosidase to the nucleus in yeast.
1984,
Pubmed
Hartman,
Beta poly(L-lysine): a model system for biological self-assembly.
1974,
Pubmed
Hartman,
Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The three structural regions of the histone H1 molecule.
1977,
Pubmed
Horowitz,
Cytoplasmic exclusion as a basis for asymmetric nucleocytoplasmic solute distributions.
1976,
Pubmed
Johns,
The electrophoresis of histones in polyacrylamide gel and their quantitative determination.
1967,
Pubmed
Kleinschmidt,
Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Lane,
Sequestration and turnover of guinea-pig milk proteins and chicken ovalbumin in Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Laskey,
Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
1977,
Pubmed
Laskey,
Chromatin assembly and transcription in eggs and oocytes of Xenopus laevis.
1978,
Pubmed
,
Xenbase
McGarry,
Intracellular distribution and degradation of immunoglobulin G and immunoglobulin G fragments injected into HeLa cells.
1983,
Pubmed
Paine,
Nucleocytoplasmic exchange of macromolecules.
1972,
Pubmed
Paine,
Nucleocytoplasmic movement of fluorescent tracers microinjected into living salivary gland cells.
1975,
Pubmed
Snell,
Kinetics and thermodynamics of the helix leads to transconformation of poly(L-lysine) and L-leucine copolymers. A compensation phenomenon.
1973,
Pubmed
Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase
van Dongen,
The accumulation of the maternal pool of histone H1A during oogenesis in Xenopus laevis.
1983,
Pubmed
,
Xenbase
