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Abstract
The orderly progression of eukaryotic cells from interphase to mitosis requires the close coordination of various nuclear and cytoplasmic events. Studies from our laboratory and others on animal cells indicate that two activities, one present mainly in mitotic cells and the other exclusively in G1-phase cells, play a pivotal role in the regulation of initiation and completion of mitosis, respectively. The purpose of this study was to investigate whether these activities are expressed in the slime mold Physarum polycephalum in which all the nuclei traverse the cell cycle in natural synchrony. Extracts were prepared from plasmodia in various phases of the cell cycle and tested for their ability to induce germinal vesicle breakdown and chromosome condensation after microinjection into Xenopus laevis oocytes. We found that extract of cells at 10-20 min before metaphase consistently induced germinal vesicle breakdown in oocytes. Preliminary characterization, including purification on a DNA-cellulose affinity column, indicated that the mitotic factors from Physarum were functionally very similar to HeLa mitotic factors. We also identified a number of mitosis-specific antigens in extracts from Physarum plasmodia, similar to those of HeLa cells, using the mitosis-specific monoclonal antibodies MPM-2 and MPM-7. Interestingly, we also observed an activity in Physarum at 45 min after metaphase (i.e., in early S phase since it has no G1) that is usually present in HeLa cells only during the G1 phase of the cell cycle. These are the first studies to show that maturation-promoting factor activity is present in Physarum during mitosis and is replaced by the G1 factor (or anti-maturation-promoting factor) activity in a postmitotic stage. A comparative study of these factors in this slime mold and in mammalian cells would be extremely valuable in further understanding their function in the regulation of eukaryotic cell cycle and their evolutionary relationship to one another.
Adlakha,
Localization of mitotic factors on metaphase chromosomes.
1982, Pubmed,
Xenbase
Adlakha,
Localization of mitotic factors on metaphase chromosomes.
1982,
Pubmed
,
Xenbase
Adlakha,
Chromosome-bound mitotic factors: release by endonucleases.
1982,
Pubmed
,
Xenbase
Adlakha,
Molecular mechanisms of the chromosome condensation and decondensation cycle in mammalian cells.
1986,
Pubmed
Adlakha,
Evidence for the presence of inhibitors of mitotic factors during G1 period in mammalian cells.
1983,
Pubmed
,
Xenbase
Adlakha,
Inactivation of mitotic factors by ultraviolet irradiation of HeLa cells in mitosis.
1984,
Pubmed
,
Xenbase
Adlakha,
Partial purification and characterization of mitotic factors from HeLa cells.
1985,
Pubmed
,
Xenbase
Al-Bader,
G2 phase-specific proteins of HeLa cells.
1978,
Pubmed
Baserga,
The cell cycle.
1981,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Burke,
A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
1986,
Pubmed
Davis,
Monoclonal antibodies to mitotic cells.
1983,
Pubmed
,
Xenbase
Drury,
Method for the preparation of active maturation promoting factor (MPF) from in vitro matured oocytes of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
Ford,
Maturation promoting factor and cell cycle regulation.
1985,
Pubmed
,
Xenbase
Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed
Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase
Halleck,
Characterization of protein kinases in mitotic and meiotic cell extracts.
1984,
Pubmed
,
Xenbase
Halleck,
Injected mitotic extracts induce condensation of interphase chromatin.
1984,
Pubmed
,
Xenbase
Johnson,
Mammalian cell fusion: induction of premature chromosome condensation in interphase nuclei.
1970,
Pubmed
Kai,
Molecular cloning of a human gene that regulates chromosome condensation and is essential for cell proliferation.
1986,
Pubmed
Karsenti,
Interconversion of metaphase and interphase microtubule arrays, as studied by the injection of centrosomes and nuclei into Xenopus eggs.
1984,
Pubmed
,
Xenbase
Karsenti,
Phosphorylation changes associated with the early cell cycle in Xenopus eggs.
1987,
Pubmed
,
Xenbase
Kishimoto,
Generality of the action of various maturation-promoting factors.
1982,
Pubmed
,
Xenbase
Laffler,
The tubulin and histone genes of Physarum polycephalum: models for cell cycle-regulated gene expression.
1986,
Pubmed
Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
,
Pubmed
Lohka,
Metaphase protein phosphorylation in Xenopus laevis eggs.
1987,
Pubmed
,
Xenbase
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase
Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase
MITTERMAYER,
THE EFFECT OF ACTINOMYCIN D ON THE TIMING OF MITOSIS IN PHYSARUM POLYCEPHALUM.
1965,
Pubmed
Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed
Masui,
Oocyte maturation.
1979,
Pubmed
Mazia,
The cell cycle.
1974,
Pubmed
Miake-Lye,
Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Miake-Lye,
Induction of early mitotic events in a cell-free system.
1985,
Pubmed
,
Xenbase
Murray,
Cell biology. Cyclins in meiosis and mitosis.
,
Pubmed
,
Xenbase
Murray,
Cell cycle control. A cycle is a cycle is a cycle.
,
Pubmed
Nelkin,
Protein factor(s) from mitotic CHO cells induce meiotic maturation in Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase
Newport,
Regulation of the cell cycle during early Xenopus development.
1984,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase
Newport,
Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes.
1987,
Pubmed
,
Xenbase
Nguyen-Gia,
Partial purification of the maturation-promoting factor MPF from unfertilized eggs of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Pardee,
Animal cell cycle.
1978,
Pubmed
RAO,
HELA CELLS: EFFECTS OF TEMPERATURE ON THE LIFE CYCLE.
1965,
Pubmed
Rao,
Mitotic synchrony in mammalian cells treated with nitrous oxide at high pressure.
1968,
Pubmed
Rosenthal,
Selective translation of mRNA controls the pattern of protein synthesis during early development of the surf clam, Spisula solidissima.
1980,
Pubmed
Russell,
Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog.
1987,
Pubmed
Russell,
cdc25+ functions as an inducer in the mitotic control of fission yeast.
1986,
Pubmed
Sahasrabuddhe,
Phosphorylation of non-histone proteins associated with mitosis in HeLa cells.
1984,
Pubmed
,
Xenbase
Smith,
The interaction of steroids with Rana pipiens Oocytes in the induction of maturation.
1971,
Pubmed
Sunkara,
Mitotic factors from mammalian cells induce germinal vesicle breakdown and chromosome condensation in amphibian oocytes.
1979,
Pubmed
,
Xenbase
Sunkara,
Mitotic factors from mammalian cells: a preliminary characterization.
1979,
Pubmed
,
Xenbase
Suprynowicz,
A fractionated cell-free system for analysis of prophase nuclear disassembly.
1986,
Pubmed
Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Wasserman,
A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization.
1976,
Pubmed
,
Xenbase
Weintraub,
[Demonstration of maturation promoting factor activity in Saccharomyces cerevisiae].
1982,
Pubmed
,
Xenbase
Woodland,
Changes in the polysome content of developing Xenopus laevis embryos.
1974,
Pubmed
,
Xenbase
Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Wu,
Partial purification and characterization of the maturation-promoting factor from eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Zierler,
Stockpiling of DNA polymerases during oogenesis and embryogenesis in the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase