XB-ART-26958
J Histochem Cytochem
1989 Feb 01;372:273-6.
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Method for epitope selection of antisera for immunohistology.
Abstract
Polyclonal anti-laminin serum was affinity-purified on paraformaldehyde-fixed laminin on a nitrocellulose filter. The purified antibodies were tested for their specificity in immunohistological stainings on frozen sections of paraformaldehyde-fixed tissue. As compared to the initial polyclonal serum, the purified antibodies increased the specificity of antigen detection, since all background caused by nonspecific reactions was eliminated. This technique promises to be very useful for immunohistological analysis using light and electron microscopy.
PubMed ID: 2463302
Article link: J Histochem Cytochem