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Nature
2012 Aug 23;4887412:531-4. doi: 10.1038/nature11285.
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NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds.
Nour-Eldin HH
,
Andersen TG
,
Burow M
,
Madsen SR
,
Jørgensen ME
,
Olsen CE
,
Dreyer I
,
Hedrich R
,
Geiger D
,
Halkier BA
.
Abstract
In plants, transport processes are important for the reallocation of defence compounds to protect tissues of high value, as demonstrated in the plant model Arabidopsis, in which the major defence compounds, glucosinolates, are translocated to seeds on maturation. The molecular basis for long-distance transport of glucosinolates and other defence compounds, however, remains unknown. Here we identify and characterize two members of the nitrate/peptide transporter family, GTR1 and GTR2, as high-affinity, proton-dependent glucosinolate-specific transporters. The gtr1 gtr2 double mutant did not accumulate glucosinolates in seeds and had more than tenfold over-accumulation in source tissues such as leaves and silique walls, indicating that both plasma membrane-localized transporters are essential for long-distance transport of glucosinolates. We propose that GTR1 and GTR2 control the loading of glucosinolates from the apoplasm into the phloem. Identification of the glucosinolate transporters has agricultural potential as a means to control allocation of defence compounds in a tissue-specific manner.
Balbi,
Jasmonate signalling network in Arabidopsis thaliana: crucial regulatory nodes and new physiological scenarios.
2008, Pubmed
Balbi,
Jasmonate signalling network in Arabidopsis thaliana: crucial regulatory nodes and new physiological scenarios.
2008,
Pubmed
Bednarek,
A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense.
2009,
Pubmed
Brown,
Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana.
2003,
Pubmed
Chen,
Long-distance phloem transport of glucosinolates in Arabidopsis.
2001,
Pubmed
Ellerbrock,
Contribution of glucosinolate transport to Arabidopsis defense responses.
2007,
Pubmed
Fan,
The Arabidopsis nitrate transporter NRT1.7, expressed in phloem, is responsible for source-to-sink remobilization of nitrate.
2009,
Pubmed
Geu-Flores,
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products.
2007,
Pubmed
Gottwald,
Genetic evidence for the in planta role of phloem-specific plasma membrane sucrose transporters.
2000,
Pubmed
Halkier,
Biology and biochemistry of glucosinolates.
2006,
Pubmed
Koroleva,
Identification of a new glucosinolate-rich cell type in Arabidopsis flower stalk.
2000,
Pubmed
Nour-Eldin,
Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.
2006,
Pubmed
Nørholm,
A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering.
2010,
Pubmed
Townsend,
Reduced terpene levels in cottonseed add food to fiber.
2007,
Pubmed
Vert,
IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth.
2002,
Pubmed
Wu,
Metabolic engineering of natural products in plants; tools of the trade and challenges for the future.
2008,
Pubmed
Zhang,
Metabolite profiling of Arabidopsis seedlings in response to exogenous sinalbin and sulfur deficiency.
2011,
Pubmed
Züst,
Using knockout mutants to reveal the growth costs of defensive traits.
2011,
Pubmed
