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Chromosoma
1988 Jan 01;963:187-96. doi: 10.1007/bf00302357.
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Structural analysis of the mitotic cycle in pre-gastrula Xenopus embryos.
Montag M
,
Spring H
,
Trendelenburg MF
.
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The long-known phenomenon of karyomere (chromosome vesicle) formation at early telophase of the nuclear cycle during early embryogenesis of a wide range of organisms including amphibians (Rubaschkin 1905; for review, see Richards 1917) was investigated in the early cleavage cycles of Xenopus laevis embryos before the mid blastula transition. Embryos were fixed and Epon embedded at successive time intervals and consecutive thick (3 micron) and ultrathin sections cut. Using conventional light microscopy at low magnification as well as phase and/or interference contrast video microscopy at high magnification, a substantial amount of information could be obtained from the analysis of optical sections in thick-sectioned material. In addition, details of the ultrastructural organization could be analysed from corresponding ultrathin sections by electron microscopy. The light microscopic analysis of serial thick sections allowed precise determination of the arrangement and sizes of telophase karyomere structures during the embryonic nuclear division cycle. It was found that small, widely spaced 1st order karyomeres fuse to larger (2nd order) karyomeres which then progressively exhibit lateral fusion of neighbouring karyomeres. The final coalescence of adjacent karyomeres marks the onset of the reorganization of the typical interphase nuclear structure. The data are discussed with regard to the occurrence of karyomeres during the embryonic nuclear cycle of arthropods, dipteran insects, and echinoderms as well as recent progress in the use of Xenopus egg extracts for in vitro assembly of nuclear structures around protein-free DNA.
Allen,
New observations on cell architecture and dynamics by video-enhanced contrast optical microscopy.
1985, Pubmed
Allen,
New observations on cell architecture and dynamics by video-enhanced contrast optical microscopy.
1985,
Pubmed
Allen,
Video-enhanced microscopy with a computer frame memory.
1983,
Pubmed
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Callan,
The lampbrush chromosomes of Xenopus laevis: preparation, identification, and distribution of 5S DNA sequences.
1987,
Pubmed
,
Xenbase
Dawid,
Gene expression in Xenopus embryogenesis.
1985,
Pubmed
,
Xenbase
Emanuelsson,
Karyomeres in early cleavage embryos of ophryotrocha labronica lagreca and bacci.
1973,
Pubmed
Fisher,
Disassembly and reassembly of nuclei in cell-free systems.
1987,
Pubmed
Foe,
Reversible chromosome condensation induced in Drosophila embryos by anoxia: visualization of interphase nuclear organization.
1985,
Pubmed
Franke,
The nuclear envelope and the architecture of the nuclear periphery.
1981,
Pubmed
Fux,
Chromosome elimination in Heteropeza pygmaea. II. Ultrastructure of the spindle apparatus.
1974,
Pubmed
Hausen,
Distribution of nuclear proteins during maturation of the Xenopus oocyte.
1985,
Pubmed
,
Xenbase
Ito,
Ultrastructure and 3H-thymidine incorporation by chromosome vesicles in sea urchin embryos.
1981,
Pubmed
Kalt,
A study of fixation of early amphibian embryos for electron microscopy.
1971,
Pubmed
,
Xenbase
Longo,
An ultrastructural analysis of mitosis and cytokinesis in the zygote of the sea urchin, Arbacia punctulata.
1972,
Pubmed
Maul,
Nuclear pore complexes. Elimination and reconstruction during mitosis.
1977,
Pubmed
Newmeyer,
In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.
1986,
Pubmed
,
Xenbase
Newmeyer,
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
1986,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase
Signoret,
[Determination, by tritiated thymidine incorporation, of the stages of cellular cycle in the axolotl germ in synchronous period of segmentation].
1974,
Pubmed
Stafstrom,
Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophila embryo.
1984,
Pubmed
Trendelenburg,
DNA injections into Xenopus embryos: fate of injected DNA in relation to formation of embryonic nuclei.
1986,
Pubmed
,
Xenbase
Tröster,
Structural organization of an active, chromosomal nucleolar organizer region (NOR) identified by light microscopy, and subsequent TEM and STEM electron microscopy.
1985,
Pubmed
Tymowska,
Karyotype analysis of Xenopus muelleri (Peters) and Xenopus laevis (Daudin), Pipidae.
1972,
Pubmed
,
Xenbase
Welter,
Nuclear reformation following metaphase in HeLa S3 cells: three-dimensional visualization of chromatid rearrangements.
1985,
Pubmed
Zatsepina,
Nuclear envelope formation around metaphase chromosomes: chromosome decondensation and nuclear envelope reconstitution during mitosis.
1982,
Pubmed
