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Development 1999 Aug 01;12615:3371-80. doi: 10.1242/dev.126.15.3371.
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Post-transcriptional regulation of Xwnt-8 expression is required for normal myogenesis during vertebrate embryonic development.

Tian Q , Nakayama T , Dixon MP , Christian JL .

The Xenopus Wnt-8 gene is transiently expressed in ventral and lateral mesoderm during gastrulation and plays a critical role in patterning these tissues. In the current study, we show that the spatial and temporal pattern of expression of endogenous Xwnt-8 is regulated, in part, at a post-transcriptional level. We have identified a novel sequence element in the 3' untranslated region of the Xwnt-8 RNA that controls the polyadenylation status of reporter and endogenous Xwnt-8 RNAs, directs rapid RNA degradation beginning precisely at the early gastrula stage, and represses translation of transcripts throughout development. Expression of endogenous Xwnt-8 is normally downregulated within lateral (presomitic) mesoderm following gastrulation. We demonstrate that rapid degradation of Xwnt-8 transcripts, mediated by these regulatory elements in the 3' untranslated region, is essential to this process and that downregulation is required to prevent overcommitment of somitic cells to a myogenic fate. These studies demonstrate a role for post-transcriptional regulation of zygotic gene expression in vertebrate embryonic patterning.

PubMed ID: 10393116
Article link: Development
Grant support: [+]

Species referenced: Xenopus
Genes referenced: acta4 actc1 actl6a hoxa9 hoxb9 hoxc9 myc ncam1 nog snai2 twist1 uts2r vim vim.2 wnt8a

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