Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Dev Cell 2009 Apr 01;164:517-27. doi: 10.1016/j.devcel.2009.02.007.
Show Gene links Show Anatomy links

The miR-430/427/302 family controls mesendodermal fate specification via species-specific target selection.

Rosa A , Spagnoli FM , Brivanlou AH .

The role of microRNAs in embryonic cell fate specification is largely unknown. In vertebrates, the miR-430/427/302 family shows a unique expression signature and is exclusively expressed during early embryogenesis. Here, we comparatively address the embryonic function of miR-302 in human embryonic stem cells (hESCs) and its ortholog miR-427 in Xenopus laevis. Interestingly, we found that this miRNA family displays species-specific target selection among ligands of the Nodal pathway, with a striking conservation of the inhibitors, Lefties, but differential targeting of the activators, Nodals. The Nodal pathway plays a crucial role in germ layer specification. Accordingly, by gain and loss of function experiments in hESCs, we show that miR-302 promotes the mesendodermal lineage at the expense of neuroectoderm formation. Similarly, depletion of miR-427 in Xenopus embryos hinders the organizer formation and leads to severe dorsal mesodermal patterning defects. These findings suggest a crucial role for the miR-430/427/302 family in vertebrate embryogenesis by controlling germ layer specification.

PubMed ID: 19386261
Article link: Dev Cell

Species referenced: Xenopus laevis
Genes referenced: chrd gsc lefty nodal nodal1 odc1 tbxt
Morpholinos: lefty MO5

Article Images: [+] show captions
References :
Ketting, Semiconserved regulation of mesendoderm differentiation by microRNAs. 2009, Pubmed, Xenbase