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Transgenic Res
2012 Dec 01;216:1265-77. doi: 10.1007/s11248-012-9600-8.
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Members of rice plasma membrane intrinsic proteins subfamily are involved in arsenite permeability and tolerance in plants.
Mosa KA
,
Kumar K
,
Chhikara S
,
Mcdermott J
,
Liu Z
,
Musante C
,
White JC
,
Dhankher OP
.
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Rice accumulates high level of arsenic (As) in its edible parts and thus plays an important role in the transfer of As into the food chain. However, the mechanisms of As uptake and its detoxification in rice are not well understood. Recently, members of the Nodulin 26-like intrinsic protein (NIP) subfamily of plant aquaporins were shown to transport arsenite in rice and Arabidopsis. Here we report that members of the rice plasma membrane intrinsic protein (PIP) subfamily are also involved in As tolerance and transport. Based on the homology search with the mammalian AQP9 and yeast Fps1 arsenite transporters, we identified and cloned five rice PIP gene subfamily members. qRT-PCR analysis of PIPs in rice root and shoot tissues revealed a significant down regulation of transcripts encoding OsPIP1;2, OsPIP1;3, OsPIP2;4, OsPIP2;6, and OsPIP2;7 in response to arsenite treatment. Heterologous expression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Xenopus laevis oocytes significantly increased the uptake of arsenite. Overexpression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Arabidopsis yielded enhanced arsenite tolerance and higher biomass accumulation. Further, these transgenic plants showed no significant accumulation of As in shoot and root tissues in long term uptake assays. Whereas, short duration exposure to arsenite caused both active influx and efflux of As in the roots. The data suggests a bidirectional arsenite permeability of rice PIPs in plants. These rice PIPs genes will be highly useful for engineering important food and biofuel crops for enhanced crop productivity on contaminated soils without increasing the accumulation of toxic As in the biomass or edible tissues.
Abedin,
Arsenic accumulation and metabolism in rice (Oryza sativa L.).
2002, Pubmed
Abedin,
Arsenic accumulation and metabolism in rice (Oryza sativa L.).
2002,
Pubmed
Aharon,
Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress.
2003,
Pubmed
Alexandersson,
Transcriptional regulation of aquaporins in accessions of Arabidopsis in response to drought stress.
2010,
Pubmed
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Bechtold,
In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.
1998,
Pubmed
Bentley,
Microbial methylation of metalloids: arsenic, antimony, and bismuth.
2002,
Pubmed
Bienert,
A subgroup of plant aquaporins facilitate the bi-directional diffusion of As(OH)3 and Sb(OH)3 across membranes.
2008,
Pubmed
Chaumont,
Regulation of plant aquaporin activity.
2005,
Pubmed
Dallagnol,
Defective active silicon uptake affects some components of rice resistance to brown spot.
2009,
Pubmed
Dhankher,
Arsenic metabolism in plants: an inside story.
2005,
Pubmed
Dhankher,
Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma-glutamylcysteine synthetase expression.
2002,
Pubmed
Fitzpatrick,
The involvement of aquaglyceroporins in transport of boron in barley roots.
2009,
Pubmed
Guo,
Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family.
2006,
Pubmed
IARC Working Group on the Evaluation of Carcinogenic Risks to Humans,
Some drinking-water disinfectants and contaminants, including arsenic. Monographs on chloramine, chloral and chloral hydrate, dichloroacetic acid, trichloroacetic acid and 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone.
2004,
Pubmed
Isayenkov,
The Arabidopsis thaliana aquaglyceroporin AtNIP7;1 is a pathway for arsenite uptake.
2008,
Pubmed
Jang,
An expression analysis of a gene family encoding plasma membrane aquaporins in response to abiotic stresses in Arabidopsis thaliana.
2004,
Pubmed
Jang,
Transgenic Arabidopsis and tobacco plants overexpressing an aquaporin respond differently to various abiotic stresses.
2007,
Pubmed
Johanson,
The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants.
2001,
Pubmed
Johansson,
The role of aquaporins in cellular and whole plant water balance.
2000,
Pubmed
,
Xenbase
Juhasz,
Toxicity issues associated with geogenic arsenic in the groundwater-soil-plant-human continuum.
2003,
Pubmed
Kamiya,
NIP1;1, an aquaporin homolog, determines the arsenite sensitivity of Arabidopsis thaliana.
2009,
Pubmed
,
Xenbase
Li,
Characterization of OsPIP2;7, a water channel protein in rice.
2008,
Pubmed
,
Xenbase
Li,
The rice aquaporin Lsi1 mediates uptake of methylated arsenic species.
2009,
Pubmed
,
Xenbase
Lian,
The role of aquaporin RWC3 in drought avoidance in rice.
2004,
Pubmed
,
Xenbase
Liu,
Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9.
2002,
Pubmed
,
Xenbase
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Ma,
Transporters of arsenite in rice and their role in arsenic accumulation in rice grain.
2008,
Pubmed
,
Xenbase
Ma,
Silicon uptake and accumulation in higher plants.
2006,
Pubmed
Ma,
A silicon transporter in rice.
2006,
Pubmed
Meharg,
Geographical variation in total and inorganic arsenic content of polished (white) rice.
2009,
Pubmed
Meharg,
Arsenic in rice--understanding a new disaster for South-East Asia.
2004,
Pubmed
Meharg,
Arsenic contamination of Bangladesh paddy field soils: implications for rice contribution to arsenic consumption.
2003,
Pubmed
Meng,
As(III) and Sb(III) uptake by GlpF and efflux by ArsB in Escherichia coli.
2004,
Pubmed
Miwa,
Homeostasis of the structurally important micronutrients, B and Si.
2009,
Pubmed
Rosen,
Biochemistry of arsenic detoxification.
2002,
Pubmed
Sakurai,
Identification of 33 rice aquaporin genes and analysis of their expression and function.
2005,
Pubmed
Thompson,
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
1997,
Pubmed
Weig,
The major intrinsic protein family of Arabidopsis has 23 members that form three distinct groups with functional aquaporins in each group.
1997,
Pubmed
,
Xenbase
Williams,
Greatly enhanced arsenic shoot assimilation in rice leads to elevated grain levels compared to wheat and barley.
2007,
Pubmed
Wysocki,
The glycerol channel Fps1p mediates the uptake of arsenite and antimonite in Saccharomyces cerevisiae.
2001,
Pubmed
Yu,
Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery.
2006,
Pubmed
Zhao,
Arsenic as a food chain contaminant: mechanisms of plant uptake and metabolism and mitigation strategies.
2010,
Pubmed
Zhao,
Arsenic uptake and metabolism in plants.
2009,
Pubmed
Zhao,
The role of the rice aquaporin Lsi1 in arsenite efflux from roots.
2010,
Pubmed
,
Xenbase
