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EMBO J
1984 Dec 20;313:3295-301. doi: 10.1002/j.1460-2075.1984.tb02293.x.
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Human U2 and U1 RNA genes use similar transcription signals.
Westin G
,
Lund E
,
Murphy JT
,
Pettersson U
,
Dahlberg JE
.
Abstract
We have analyzed the requirements for human U2 RNA transcription by injection of cloned U2/6 RNA genes into nuclei of Xenopus laevis oocytes. Two forms of human U2 RNAs accumulate, a major species corresponding to mature-sized U2 RNA and a minor species corresponding to a 3'-extended precursor. This RNA polymerase II transcription requires only 258 and 94 bp of 5'- and 3'-flanking region sequences, respectively. Efficient U2 RNA synthesis depends on a promoter element located between positions -258 and -198. This region contains a 12-bp direct repeat which strongly resembles a comparable upstream promoter element of the human U1 RNA genes. Sequences between -258 and -198 also confer on the U2 RNA template the ability to complete with co-injected U1 RNA templates for a snRNA gene-specific transcription factor(s). Transcription of U2 RNA is reduced off templates containing an active RNA polymerase III transcription unit, presumably because of relaxation or sequestration of the DNA. In vitro transcription of the U2 RNA gene, like that of the U1 RNA gene, is initiated upstream of the point corresponding to the 5' end of in vivo synthesized RNA.
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Beck,
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Bolivar,
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
1977,
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Busch,
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Elder,
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1981,
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Eliceiri,
Small RNAs in the nucleus and cytoplasm of HeLa cells.
1976,
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Hammarström,
Genes and pseudogenes for human U2 RNA. Implications for the mechanism of pseudogene formation.
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Harland,
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,
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Lund,
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Manser,
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Marzluff,
Isolation and characterization of two linked mouse U1b small nuclear RNA genes.
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Mattaj,
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,
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Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
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Miller,
Template structural requirements for transcription in vivo by RNA polymerase II.
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,
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Murphy,
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,
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Nilsson,
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Nohga,
Comparison of RNase T1 fingerprints of U1, U2, and U3 small nuclear RNA's of HeLa cells, human normal fibroblasts, and Novikoff hepatoma cells.
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Nojima,
Genes and pseudogenes for mouse U1 and U2 small nuclear RNAs.
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,
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Pan,
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Reddy,
Primary and secondary structure of U2 snRNA.
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Roop,
Structure and expression of a chicken gene coding for U1 RNA.
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Sanger,
A two-dimensional fractionation procedure for radioactive nucleotides.
1965,
Pubmed
Silberklang,
Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
1979,
Pubmed
Skuzeski,
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
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,
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Tani,
Molecular cloning and characterization of a gene for rat U2 small nuclear RNA.
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Van Arsdell,
Human genes for U2 small nuclear RNA are tandemly repeated.
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Structural analysis of gene loci for rat U1 small nuclear RNA.
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Weiher,
Multiple point mutations affecting the simian virus 40 enhancer.
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Westin,
Human DNA sequences complementary to the small nuclear RNA U2.
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Westin,
Clustered genes for human U2 RNA.
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Zeller,
Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families.
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,
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