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We describe a method for the synthesis of microgram quantities of eucaryotic messenger RNAs. Injection into the cytoplasm of frog oocytes and addition to wheat germ extracts show that these synthetic RNAs function efficiently as messenger RNAs. We confirm that a 5' cap on the mRNA is essential for translation in injected oocytes and show that most of the 3' flanking region, including the poly A tail, can be deleted without the abolition of protein synthesis. The method of mRNA synthesis involves in vitro transcription of cDNAs which have been cloned into SP6 vectors (described in the accompanying paper). This method enables one to produce large amounts of mRNA and consequently protein from any cDNA clone.
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974, Pubmed,
Xenbase
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974,
Pubmed
,
Xenbase
Butler,
Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.
1982,
Pubmed
Cavalieri,
Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.
1977,
Pubmed
,
Xenbase
Colman,
Export of proteins from oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Darnell,
Transcription units for mRNA production in eukaryotic cells and their DNA viruses.
1979,
Pubmed
Goldberg,
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
1980,
Pubmed
Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Gurdon,
Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages.
1973,
Pubmed
,
Xenbase
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Huez,
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.
1974,
Pubmed
,
Xenbase
Huez,
Readenylation of polyadenylate-free globin messenger RNA restores its stability in vivo.
1975,
Pubmed
,
Xenbase
Krainer,
Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.
1984,
Pubmed
Krieg,
Formation of the 3' end of histone mRNA by post-transcriptional processing.
,
Pubmed
,
Xenbase
Kronenberg,
The 3' noncoding region of beta-globin mRNA is not essential for in vitro translation.
1979,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Laskey,
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
Pubmed
Lenk,
A cytoskeletal structure with associated polyribosomes obtained from HeLa cells.
1977,
Pubmed
Lockard,
Requirement for 7-methylguanosine in translation of globin mRNA in vivo.
1978,
Pubmed
,
Xenbase
Martin,
Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions.
1975,
Pubmed
Monroy,
Purification of mRNA guanylyltransferase from vaccinia virions.
1978,
Pubmed
Paterson,
Efficient translation of prokaryotic mRNAs in a eukaryotic cell-free system requires addition of a cap structure.
1979,
Pubmed
Patient,
The complete nucleotide sequence of the major adult beta globin gene of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Perry,
Processing of RNA.
1976,
Pubmed
Richter,
The mechanism for increased protein synthesis during Xenopus oocyte maturation.
1982,
Pubmed
,
Xenbase
Richter,
Differential capacity for translation and lack of competition between mRNAs that segregate to free and membrane-bound polysomes.
1981,
Pubmed
,
Xenbase
Roberts,
Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system.
1975,
Pubmed
Ruskin,
Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.
1984,
Pubmed
Taniguchi,
Human leukocyte and fibroblast interferons are structurally related.
1980,
Pubmed
Williams,
The nucleotide sequence of the major beta-globin mRNA from Xenopus laevis.
1980,
Pubmed
,
Xenbase
Yost,
A stop transfer sequence confers predictable transmembrane orientation to a previously secreted protein in cell-free systems.
1983,
Pubmed