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Translational efficiency of poliovirus mRNA: mapping inhibitory cis-acting elements within the 5' noncoding region.
Pelletier J
,
Kaplan G
,
Racaniello VR
,
Sonenberg N
.
Abstract
Poliovirus mRNA contains a long 5' noncoding region of about 750 nucleotides (the exact number varies among the three virus serotypes), which contains several AUG codons upstream of the major initiator AUG. Unlike most eucaryotic mRNAs, poliovirus does not contain a m7GpppX (where X is any nucleotide) cap structure at its 5' end and is translated by a cap-independent mechanism. To study the manner by which poliovirus mRNA is expressed, we examined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of the mRNA. In this paper we report striking translation system-specific differences in the ability of the altered mRNAs to be translated. The results suggest the existence of an inhibitory cis-acting element(s) within the 5' noncoding region of poliovirus (between nucleotides 70 and 381) which restricts mRNA translation in reticulocyte lysate, wheat germ extract, and Xenopus oocytes, but not in HeLa cell extracts. In addition, we show that HeLa cell extracts contain a trans-acting factor(s) that overcomes this restriction.
Blair,
Functional identity of a mouse ascites and a rabbit reticulocyte initiation factor required for natural mRNA translation.
1977, Pubmed
Blair,
Functional identity of a mouse ascites and a rabbit reticulocyte initiation factor required for natural mRNA translation.
1977,
Pubmed
Both,
Methylation-dependent translation of viral messenger RNAs in vitro.
1975,
Pubmed
Brown,
Initiation factor preparations from poliovirus-infected cells restrict translation in reticulocyte lysates.
1980,
Pubmed
Brown,
Translation of poliovirus RNA in vitro: changes in cleavage pattern and initiation sites by ribosomal salt wash.
1979,
Pubmed
Daniels-McQueen,
Unusual requirements for optimum translation of polio viral RNA in vitro.
1983,
Pubmed
Dorner,
In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate.
1984,
Pubmed
Flanegan,
Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.
1977,
Pubmed
Furuichi,
5'-Terminal structure and mRNA stability.
1977,
Pubmed
,
Xenbase
Hewlett,
5'-terminal structure of poliovirus polyribosomal RNA is pUp.
1976,
Pubmed
HOLLAND,
Receptor affinities as major determinants of enterovirus tissue tropisms in humans.
1961,
Pubmed
Jen,
Shutoff of HeLa cell protein synthesis by encephalomyocarditis virus and poliovirus: a comparative study.
1980,
Pubmed
Katz,
Role of the avian retrovirus mRNA leader in expression: evidence for novel translational control.
1986,
Pubmed
Kozak,
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
1983,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
La Monica,
Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing.
1986,
Pubmed
Lee,
Inactivation of cap-binding proteins accompanies the shut-off of host protein synthesis by poliovirus.
1982,
Pubmed
Lee,
A protein covalently linked to poliovirus genome RNA.
1977,
Pubmed
McClain,
Ribosomal binding sites on poliovirus RNA.
1981,
Pubmed
Nicklin,
Poliovirus polypeptide precursors: expression in vitro and processing by exogenous 3C and 2A proteinases.
1987,
Pubmed
Nobis,
Production of a monoclonal antibody against an epitope on HeLa cells that is the functional poliovirus binding site.
1985,
Pubmed
Nomoto,
The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
1976,
Pubmed
Okada,
Poliovirus infection of established human blood cell lines: relationship between the differentiation stage and susceptibility of cell killing.
1987,
Pubmed
Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed
Pelletier,
Insertion mutagenesis to increase secondary structure within the 5' noncoding region of a eukaryotic mRNA reduces translational efficiency.
1985,
Pubmed
Pelletier,
Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.
1988,
Pubmed
Phillips,
Modulation of the expression of poliovirus proteins in reticulocyte lysates.
1986,
Pubmed
Ray,
ATP-dependent unwinding of messenger RNA structure by eukaryotic initiation factors.
1985,
Pubmed
Rose,
Inhibition of translation by poliovirus: inactivation of a specific initiation factor.
1978,
Pubmed
Shih,
Cell-free synthesis and processing of the proteins of poliovirus.
1978,
Pubmed
Sonenberg,
Regulation of translation by poliovirus.
1987,
Pubmed
Weber,
Inhibition of HeLa cell messenger RNA translation by 7-methylguanosine 5'-monophosphate.
1976,
Pubmed