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XB-ART-27451
J Virol 1988 Jul 01;627:2219-27. doi: 10.1128/JVI.62.7.2219-2227.1988.
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Translational efficiency of poliovirus mRNA: mapping inhibitory cis-acting elements within the 5' noncoding region.

Pelletier J , Kaplan G , Racaniello VR , Sonenberg N .


Abstract
Poliovirus mRNA contains a long 5' noncoding region of about 750 nucleotides (the exact number varies among the three virus serotypes), which contains several AUG codons upstream of the major initiator AUG. Unlike most eucaryotic mRNAs, poliovirus does not contain a m7GpppX (where X is any nucleotide) cap structure at its 5' end and is translated by a cap-independent mechanism. To study the manner by which poliovirus mRNA is expressed, we examined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of the mRNA. In this paper we report striking translation system-specific differences in the ability of the altered mRNAs to be translated. The results suggest the existence of an inhibitory cis-acting element(s) within the 5' noncoding region of poliovirus (between nucleotides 70 and 381) which restricts mRNA translation in reticulocyte lysate, wheat germ extract, and Xenopus oocytes, but not in HeLa cell extracts. In addition, we show that HeLa cell extracts contain a trans-acting factor(s) that overcomes this restriction.

PubMed ID: 2836606
PMC ID: PMC253356
Article link: J Virol
Grant support: [+]


References [+] :
Blair, Functional identity of a mouse ascites and a rabbit reticulocyte initiation factor required for natural mRNA translation. 1977, Pubmed