Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Does 3'-terminal poly(A) stabilize human fibroblast interferon mRNA in oocytes of Xenopus laevis?
Sehgal PB
,
Soreq H
,
Tamm I
.
???displayArticle.abstract???
Polynucleotide phosphorylase (polyribonucleotide:orthophosphate nucleotidyltransferase, EC 2.7.7.8) purified from Escherichia coli was used enzymatically to deadenylate polyadenylated human fibroblast interferon mRNA preparations obtained from human diploid fibroblasts (FS-4 strain) induced by poly(I)-poly(C) (20 microgram/ml) in the presence of cycloheximide (50 microgram/ml, 4 hr). Both the polyadenylated and the deadenylated interferon mRNA preparations were translated into biologically active human interferon when injected into oocytes of Xenopus laevis. In the oocytes the functional stability of deadenylated interferon mRNA was indistinguishable from that of polyadenylated interferon mRNA.
Armstrong,
Semi-micro, dye-binding assay for rabbit interferon.
1971, Pubmed
Armstrong,
Semi-micro, dye-binding assay for rabbit interferon.
1971,
Pubmed
Cavalieri,
Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.
1977,
Pubmed
,
Xenbase
EAGLE,
Amino acid metabolism in mammalian cell cultures.
1959,
Pubmed
Grosfeld,
Membrane associated cytoplasmic mRNA in Artemia salina; functional and physical changes during development.
1977,
Pubmed
Havell,
Production of high-titered interferon in cultures of human diploid cells.
1972,
Pubmed
Huez,
Functional stabilisation of HeLa cell histone messenger RNAs injected into Xenopus oocytes by 3'-OH polyadenylation.
1978,
Pubmed
,
Xenbase
Huez,
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.
1974,
Pubmed
,
Xenbase
Huez,
Readenylation of polyadenylate-free globin messenger RNA restores its stability in vivo.
1975,
Pubmed
,
Xenbase
Jelinek,
Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNA.
1973,
Pubmed
Marbaix,
Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.
1975,
Pubmed
,
Xenbase
Peacock,
Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
1968,
Pubmed
Sehgal,
Interferon messenger RNA content of human fibroblasts during induction, shutoff, and superinduction of interferon production.
1977,
Pubmed
,
Xenbase
Sehgal,
The inhibition by DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) of hnRNA and mRNA production in HeLa cells.
1976,
Pubmed
Sehgal,
Superinduction of human fibroblast interferon production: further evidence for increased stability of interferon mRNA.
1978,
Pubmed
Sehgal,
Enhancement of human interferon production by neutral red and chloroquine: analysis of inhibition of protein degradation and macromolecular synthesis.
1975,
Pubmed
Sehgal,
Human interferon production: superinduction by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole.
1975,
Pubmed
Soreq,
Purification and characterization of polynucleotide phosphorylase from Escherichia coli. Probe for the analysis of 3' sequences of RNA.
1977,
Pubmed
Soreq,
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.
1974,
Pubmed
Tamm,
Halobenzimidazole ribosides and RNA synthesis of cells and viruses.
1978,
Pubmed
Tamm,
Definition of subclasses of nucleoplasmic RNA.
1977,
Pubmed
Tamm,
Action of dichlorobenzimidazole riboside on RNA synthesis in L-929 and HeLa cells.
1976,
Pubmed
Watanabe,
Regulation of transcription of the Reovirus genome.
1968,
Pubmed
