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The two functionally distinct amino termini of chicken c-ets-1 products arise from alternative promoter usage.
Crepieux P
,
Leprince D
,
Flourens A
,
Albagli O
,
Ferreira E
,
Stéhelin D
.
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The chicken c-ets-1 locus gives rise to two distinct transcription factors differing by structurally and functionally unrelated N-termini. p54c-ets-1 shows a striking phylogenetic conservation from Xenopus to humans, while p68c-ets-1, the cellular counterpart of the E26-derived v-ets oncogene, is apparently restricted to avian and reptilian species. In the chick embryo, both mRNAs are expressed in a wide array of tissues of mesodermal origin; however, in the embryo and after hatching, p68c-ets-1 is excluded from lymphoid cells where p54c-ets-1 accumulates. In this report, we define the basis of the differential expression of the chicken c-ets-1 products to assess their different potentials as transcription factors. We demonstrate that the two distinct N-termini arise from alternative promoter usage within the chicken c-ets-1 locus. Examination of both promoters reveals that transcription initiates from multiple sites, consistent with the absence of TATA and CAAT elements. Of these two regulatory regions, only the one that initiates the p54c-ets-1 mRNA synthesis is of the G + C-rich type, and its organization is conserved in humans. The avian-specific p68c-ets-1 promoter activity was enhanced by its own product. In addition, we identify numerous potential binding sites for lymphoid-specific transcription factors that might contribute to a tight repressor effect in lymphoid tissues.
Albagli,
Phylogeny of the p68c-ets-1 amino-terminal transactivating domain reveals some highly conserved structural features.
1992, Pubmed
Albagli,
Phylogeny of the p68c-ets-1 amino-terminal transactivating domain reveals some highly conserved structural features.
1992,
Pubmed
Allen,
Functional dissection of the lck proximal promoter.
1992,
Pubmed
Anderson,
A conserved sequence in the T-cell receptor beta-chain promoter region.
1988,
Pubmed
Biggin,
Transcription factors that activate the Ultrabithorax promoter in developmentally staged extracts.
1988,
Pubmed
Dalton,
Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element.
1992,
Pubmed
de Wet,
Firefly luciferase gene: structure and expression in mammalian cells.
1987,
Pubmed
Duterque-Coquillaud,
Cloning and expression of chicken p54c-ets cDNAs: the first p54c-ets coding exon is located into the 40.0 kbp genomic domain unrelated to v-ets.
1988,
Pubmed
Evans,
trans-Activation of a globin promoter in nonerythroid cells.
1991,
Pubmed
Evans,
A promoter of the rat insulin-like growth factor II gene consists of minimal control elements.
1988,
Pubmed
Gegonne,
Multiple domains for the chicken cellular sequences homologous to the v-ets oncogene of the E26 retrovirus.
1987,
Pubmed
Ghysdael,
Identification and preferential expression in thymic and bursal lymphocytes of a c-ets oncogene-encoded Mr 54,000 cytoplasmic protein.
1986,
Pubmed
Gouilleux,
Cooperation between structural elements in hormono-regulated transcription from the mouse mammary tumor virus promoter.
1991,
Pubmed
Gunther,
Sequence-specific DNA binding of the proto-oncoprotein ets-1 defines a transcriptional activator sequence within the long terminal repeat of the Moloney murine sarcoma virus.
1990,
Pubmed
Hannink,
Structure and autoregulation of the c-rel promoter.
1990,
Pubmed
Hannon,
Structure and promoter activity of the gene for the erythroid transcription factor GATA-1.
1991,
Pubmed
Hanvey,
Intramolecular DNA triplexes in supercoiled plasmids.
1988,
Pubmed
Hipskind,
Ets-related protein Elk-1 is homologous to the c-fos regulatory factor p62TCF.
,
Pubmed
Ho,
Sequence-specific binding of human Ets-1 to the T cell receptor alpha gene enhancer.
1990,
Pubmed
Imagawa,
Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP.
1987,
Pubmed
Jorcyk,
The human ETS1 gene: genomic structure, promoter characterization and alternative splicing.
1991,
Pubmed
Karim,
The ETS-domain: a new DNA-binding motif that recognizes a purine-rich core DNA sequence.
1990,
Pubmed
Klemsz,
The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene.
1990,
Pubmed
Ko,
Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer.
1991,
Pubmed
Laudet,
Evolution of the ets gene family.
1993,
Pubmed
Lautenberger,
Genomic dispersal of the ets gene family during metazoan evolution.
1992,
Pubmed
,
Xenbase
Leiden,
Transcriptional regulation during T-cell development: the alpha TCR gene as a molecular model.
1992,
Pubmed
Leprince,
A putative second cell-derived oncogene of the avian leukaemia retrovirus E26.
,
Pubmed
Leprince,
The chicken cellular progenitor of the v-ets oncogene, p68c-ets-1, is a nuclear DNA-binding protein not expressed in lymphoid cells of the spleen.
1990,
Pubmed
Leprince,
Alternative splicing within the chicken c-ets-1 locus: implications for transduction within the E26 retrovirus of the c-ets proto-oncogene.
1988,
Pubmed
Macleod,
The ets gene family.
1992,
Pubmed
Majérus,
Serum, AP-1 and Ets-1 stimulate the human ets-1 promoter.
1992,
Pubmed
Mavrothalassitis,
Molecular and functional characterization of the promoter of ETS2, the human c-ets-2 gene.
1990,
Pubmed
Meeker,
The human Pim-1 gene is selectively transcribed in different hemato-lymphoid cell lines in spite of a G + C-rich housekeeping promoter.
1990,
Pubmed
Nunn,
Tripartite structure of the avian erythroblastosis virus E26 transforming gene.
,
Pubmed
Oka,
Structural and functional analysis of the regulatory sequences of the ets-1 gene.
1991,
Pubmed
Prosser,
A phorbol ester response element within the human T-cell receptor beta-chain enhancer.
1992,
Pubmed
Quéva,
p54c-ets-1 and p68c-ets-1, the two transcription factors encoded by the c-ets-1 locus, are differentially expressed during the development of the chick embryo.
1993,
Pubmed
Rao,
elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sayers,
5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis.
1988,
Pubmed
Schneikert,
Two independent activation domains in c-Ets-1 and c-Ets-2 located in non-conserved sequences of the ets gene family.
1992,
Pubmed
Selden,
Human growth hormone as a reporter gene in regulation studies employing transient gene expression.
1986,
Pubmed
Seth,
The c-ets-1 proto-oncogene has oncogenic activity and is positively autoregulated.
1990,
Pubmed
Smale,
The "initiator" as a transcription control element.
1989,
Pubmed
Thompson,
cis-acting sequences required for inducible interleukin-2 enhancer function bind a novel Ets-related protein, Elf-1.
1992,
Pubmed
van de Wetering,
Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box.
1991,
Pubmed
Virbasius,
Transcriptional activation through ETS domain binding sites in the cytochrome c oxidase subunit IV gene.
1991,
Pubmed
Wang,
Evolutionarily conserved Ets family members display distinct DNA binding specificities.
1992,
Pubmed
Wasylyk,
The c-ets proto-oncogenes encode transcription factors that cooperate with c-Fos and c-Jun for transcriptional activation.
1990,
Pubmed
Wasylyk,
The c-Ets oncoprotein activates the stromelysin promoter through the same elements as several non-nuclear oncoproteins.
1991,
Pubmed
Waterman,
A thymus-specific member of the HMG protein family regulates the human T cell receptor C alpha enhancer.
1991,
Pubmed
Watson,
Mammalian ets-1 and ets-2 genes encode highly conserved proteins.
1988,
Pubmed
,
Xenbase
Woods,
Identification of nucleotide preferences in DNA sequences recognised specifically by c-Ets-1 protein.
1992,
Pubmed
Ye,
Identification of the promoter region of human interleukin 1 type I receptor gene: multiple initiation sites, high G+C content, and constitutive expression.
1993,
Pubmed
