Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cytosine methylation in CTF and Sp1 recognition sites of an HSV tk promoter: effects on transcription in vivo and on factor binding in vitro.
Ben-Hattar J
,
Beard P
,
Jiricny J
.
Abstract
We methylated specific cytosine residues within or immediately around the CTF and Sp1 binding sites of the Herpes simplex virus thymidine kinase promoter. The efficiency of transcription in vivo was reduced at least 50-fold compared with transcription from the unmethylated promoter. However, methylation within the CTF recognition site had no effect on the affinity of CTF for this site in vitro. Methylation of the Sp1 site resulted in only a small decrease in the affinity of this factor for its recognition site. In vivo studies showed that the same gene inserted in different vector DNAs was regulated differently by methylation in the promoter. These results show that cytosine methylation can inhibit transcription by a mechanism other than directly blocking the binding of transcription factors.
Becker,
Genomic footprinting reveals cell type-specific DNA binding of ubiquitous factors.
1987, Pubmed
Becker,
Genomic footprinting reveals cell type-specific DNA binding of ubiquitous factors.
1987,
Pubmed
Ben-Hattar,
Methylation of single CpG dinucleotides within a promoter element of the Herpes simplex virus tk gene reduces its transcription in vivo.
1988,
Pubmed
,
Xenbase
Briggs,
Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.
1986,
Pubmed
Buschhausen,
Inhibition of herpes simplex thymidine kinase gene expression by DNA methylation is an indirect effect.
1985,
Pubmed
Buschhausen,
Chromatin structure is required to block transcription of the methylated herpes simplex virus thymidine kinase gene.
1987,
Pubmed
Busslinger,
DNA methylation and the regulation of globin gene expression.
1983,
Pubmed
Chodosh,
Human CCAAT-binding proteins have heterologous subunits.
1988,
Pubmed
Collins,
Alterations in DNA helix stability due to base modifications can be evaluated using denaturing gradient gel electrophoresis.
1987,
Pubmed
Dobrzanski,
Inactivation by sequence-specific methylations of adenovirus promoters in a cell-free transcription system.
1988,
Pubmed
Dorn,
A multiplicity of CCAAT box-binding proteins.
1987,
Pubmed
Feavers,
Interaction of two nonhistone proteins with the estradiol response element of the avian vitellogenin gene modulates the binding of estradiol-receptor complex.
1987,
Pubmed
Fox,
The effect of HhaI methylation on DNA local structure.
1986,
Pubmed
Fried,
CAP and RNA polymerase interactions with the lac promoter: binding stoichiometry and long range effects.
1983,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Graves,
Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene.
1986,
Pubmed
Harrington,
Cytosine methylation does not affect binding of transcription factor Sp1.
1988,
Pubmed
Höller,
Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated.
1988,
Pubmed
Jackson,
O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation.
1988,
Pubmed
Janson,
Identification of proteins interacting with the enhancer of human U2 small nuclear RNA genes.
1987,
Pubmed
Jiricny,
Oligonucleotide duplexes containing inosine, 7-deazainosine, tubercidin, nebularine and 7-deazanebularine as substrates for restriction endonucleases HindII, SalI and TaqI.
1986,
Pubmed
Jiricny,
A human 200-kDa protein binds selectively to DNA fragments containing G.T mismatches.
1988,
Pubmed
Keshet,
DNA methylation affects the formation of active chromatin.
1986,
Pubmed
Knebel-Mörsdorf,
Reactivation of the methylation-inhibited late E2A promoter of adenovirus type 2 by a strong enhancer of human cytomegalovirus.
1988,
Pubmed
Langner,
DNA methylation of three 5' C-C-G-G 3' sites in the promoter and 5' region inactivate the E2a gene of adenovirus type 2.
1984,
Pubmed
,
Xenbase
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McClelland,
The effect of sequence specific DNA methylation on restriction endonuclease cleavage.
1981,
Pubmed
McKnight,
The distal transcription signals of the herpesvirus tk gene share a common hexanucleotide control sequence.
1984,
Pubmed
,
Xenbase
McKnight,
Transcriptional selectivity of viral genes in mammalian cells.
1986,
Pubmed
Michaeli,
pBR322 DNA inhibits simian virus 40 gene expression in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase
Murchie,
Base methylation and local DNA helix stability. Effect on the kinetics of cruciform extrusion.
1989,
Pubmed
Razin,
DNA methylation patterns. Formation and function.
1984,
Pubmed
Razin,
DNA methylation and gene function.
1980,
Pubmed
Siebenlist,
E. coli RNA polymerase interacts homologously with two different promoters.
1980,
Pubmed
Singleton,
Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions.
1982,
Pubmed
Superti-Furga,
The -117 mutation in Greek HPFH affects the binding of three nuclear factors to the CCAAT region of the gamma-globin gene.
1988,
Pubmed
Watt,
Cytosine methylation prevents binding to DNA of a HeLa cell transcription factor required for optimal expression of the adenovirus major late promoter.
1988,
Pubmed
Weisshaar,
Reactivation of the methylation-inactivated late E2A promoter of adenovirus type 2 by E1A (13 S) functions.
1988,
Pubmed
,
Xenbase
Wells,
Unusual DNA structures.
1988,
Pubmed
