Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.
Contreras R
,
Cheroutre H
,
Degrave W
,
Fiers W
.
???displayArticle.abstract???
A simple and efficient method for direct in vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts were translated in vivo in Xenopus laevis oocytes and in vitro in reticulocyte and in wheat germ cell-free protein synthesizing systems.
Contreras,
Proceedings: Controlled transcription of the synthetic gene for tyrosine transfer RNA.
1976, Pubmed
Contreras,
Proceedings: Controlled transcription of the synthetic gene for tyrosine transfer RNA.
1976,
Pubmed
Contreras,
Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.
1977,
Pubmed
Contreras,
A simple apparatus for injection of nanoliter quantities into Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase
Derynck,
Isolation and structure of a human fibroblast interferon gene.
1980,
Pubmed
Derynck,
Expression of human fibroblast interferon gene in Escherichia coli.
1980,
Pubmed
Downey,
Studies on the kinetics of ribonucleic acid chain initiation and elongation.
1970,
Pubmed
Downey,
Determination of nucleotide sequences at promoter regions by the use of dinucleotides.
1971,
Pubmed
Fiers,
Complete nucleotide sequence of SV40 DNA.
1978,
Pubmed
Gheysen,
Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-t antigen gene.
1982,
Pubmed
Kleppe,
Studies on polynucleotides. CXIX. Transcription of short double-stranded deoxyribonucleic acids of defined nucleotide sequences.
1972,
Pubmed
Kozak,
How do eucaryotic ribosomes select initiation regions in messenger RNA?
1978,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Paterson,
Efficient translation of prokaryotic mRNAs in a eukaryotic cell-free system requires addition of a cap structure.
1979,
Pubmed
Remaut,
Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
1981,
Pubmed
Roberts,
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
1973,
Pubmed
Shine,
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
1974,
Pubmed
Smith,
Analysis of RNA initiated in isolated mouse myeloma nuclei using purine nucleoside 5'[gamma-S]triphosphates as affinity probes.
1978,
Pubmed
Terao,
Studies on polynucleotides. CXX. On the transcription of a synthetic 29-unit long deoxyribopolynucleotide.
1972,
Pubmed
Volckaert,
Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure.
1976,
Pubmed
Wei,
Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus.
1974,
Pubmed
Wimmer,
Sequence studies of satellite tobacco necrosis virus RNA. Isolation and characterization of a 5'-terminal trinucleotide.
1968,
Pubmed
