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Biochem J
2006 Dec 01;4002:291-301. doi: 10.1042/BJ20060490.
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CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Marquis J
,
Paillard L
,
Audic Y
,
Cosson B
,
Danos O
,
Le Bec C
,
Osborne HB
.
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CUG-BP1 [CUG-binding protein 1 also called CELF (CUG-BP1 and ETR3 like factors) 1] is a human RNA-binding protein that has been implicated in the control of splicing and mRNA translation. The Xenopus homologue [EDEN-BP (embryo deadenylation element-binding protein)] is required for rapid deadenylation of certain maternal mRNAs just after fertilization. A variety of sequence elements have been described as target sites for these two proteins but their binding specificity is still controversial. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant CUG-BP1 we selected two families of aptamers. Surface plasmon resonance and electrophoretic mobility-shift assays showed that these two families differed in their ability to bind CUG-BP1. Furthermore, the selected high-affinity aptamers form two complexes with CUG-BP1 in electrophoretic mobility assays whereas those that bind with low affinity only form one complex. The validity of the distinction between the two families of aptamers was confirmed by a functional in vivo deadenylation assay. Only those aptamers that bound CUG-BP1 with high affinity conferred deadenylation on a reporter mRNA. These high-affinity RNAs are characterized by a richness in UGU motifs. Using these binding site characteristics we identified the Xenopus maternal mRNA encoding the MAPK (mitogen-activated protein kinase) phosphatase (XCl100alpha) as a substrate for EDEN-BP. In conclusion, high-affinity CUG-BP1 binding sites are sequence elements at least 30 nucleotides in length that are enriched in combinations of U and G nucleotides and contain at least 4 UGU trinucleotide motifs. Such sequence elements are functionally competent to target an RNA for deadenylation in vivo.
Audic,
Postfertilization deadenylation of mRNAs in Xenopus laevis embryos is sufficient to cause their degradation at the blastula stage.
1997, Pubmed,
Xenbase
Audic,
Postfertilization deadenylation of mRNAs in Xenopus laevis embryos is sufficient to cause their degradation at the blastula stage.
1997,
Pubmed
,
Xenbase
Barreau,
Mammalian CELF/Bruno-like RNA-binding proteins: molecular characteristics and biological functions.
2006,
Pubmed
Bonnet-Corven,
An analysis of the sequence requirements of EDEN-BP for specific RNA binding.
2002,
Pubmed
,
Xenbase
Charlet-B,
Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.
2002,
Pubmed
Cosson,
Oligomerization of EDEN-BP is required for specific mRNA deadenylation and binding.
2006,
Pubmed
,
Xenbase
DeMaria,
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
1996,
Pubmed
Delaunay,
The Drosophila Bruno paralogue Bru-3 specifically binds the EDEN translational repression element.
2004,
Pubmed
,
Xenbase
Ezzeddine,
EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila.
2002,
Pubmed
,
Xenbase
Faustino,
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
2005,
Pubmed
Gold,
Diversity of oligonucleotide functions.
1995,
Pubmed
Good,
A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator.
2000,
Pubmed
,
Xenbase
Gotoh,
Involvement of the MAP kinase cascade in Xenopus mesoderm induction.
1995,
Pubmed
,
Xenbase
Gromak,
Antagonistic regulation of alpha-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein.
2003,
Pubmed
Grosset,
A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex.
2000,
Pubmed
Ho,
Transgenic mice expressing CUG-BP1 reproduce splicing mis-regulation observed in myotonic dystrophy.
2005,
Pubmed
Karlsson,
Biosensor analysis of drug-target interactions: direct and competitive binding assays for investigation of interactions between thrombin and thrombin inhibitors.
2000,
Pubmed
Kiledjian,
Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex.
1997,
Pubmed
Kino,
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
2004,
Pubmed
Klug,
All you wanted to know about SELEX.
1994,
Pubmed
Ladd,
The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing.
2001,
Pubmed
Ladd,
Cardiac tissue-specific repression of CELF activity disrupts alternative splicing and causes cardiomyopathy.
2005,
Pubmed
Lewis,
XCL100, an inducible nuclear MAP kinase phosphatase from Xenopus laevis: its role in MAP kinase inactivation in differentiated cells and its expression during early development.
1995,
Pubmed
,
Xenbase
Loflin,
Unraveling a cytoplasmic role for hnRNP D in the in vivo mRNA destabilization directed by the AU-rich element.
1999,
Pubmed
Mankodi,
Expanded CUG repeats trigger aberrant splicing of ClC-1 chloride channel pre-mRNA and hyperexcitability of skeletal muscle in myotonic dystrophy.
2002,
Pubmed
Michalowski,
Visualization of double-stranded RNAs from the myotonic dystrophy protein kinase gene and interactions with CUG-binding protein.
1999,
Pubmed
Moraes,
CUG-BP binds to RNA substrates and recruits PARN deadenylase.
2006,
Pubmed
,
Xenbase
Mukhopadhyay,
Coupled mRNA stabilization and translational silencing of cyclooxygenase-2 by a novel RNA binding protein, CUGBP2.
2003,
Pubmed
Myszka,
Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors.
1997,
Pubmed
Ostareck-Lederer,
Cytoplasmic regulatory functions of the KH-domain proteins hnRNPs K and E1/E2.
1998,
Pubmed
Ostareck-Lederer,
Control of mRNA translation and stability in haematopoietic cells: the function of hnRNPs K and E1/E2.
2004,
Pubmed
Paillard,
EDEN and EDEN-BP, a cis element and an associated factor that mediate sequence-specific mRNA deadenylation in Xenopus embryos.
1998,
Pubmed
,
Xenbase
Paillard,
A functional deadenylation assay identifies human CUG-BP as a deadenylation factor.
2003,
Pubmed
,
Xenbase
Paillard,
c-Jun ARE targets mRNA deadenylation by an EDEN-BP (embryo deadenylation element-binding protein)-dependent pathway.
2002,
Pubmed
,
Xenbase
Paris,
Changes in the polyadenylation of specific stable RNA during the early development of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Philips,
Disruption of splicing regulated by a CUG-binding protein in myotonic dystrophy.
1998,
Pubmed
Poleev,
A trans-acting factor, isolated by the three-hybrid system, that influences alternative splicing of the amyloid precursor protein minigene.
2000,
Pubmed
Savkur,
Aberrant regulation of insulin receptor alternative splicing is associated with insulin resistance in myotonic dystrophy.
2001,
Pubmed
Shen,
Biosensor analysis of the molecular interactions of pentosan polysulfate and of sulfated glycosaminoglycans with immobilized elastase, hyaluronidase and lysozyme using surface plasmon resonance (SPR) technology.
2003,
Pubmed
Suzuki,
Regulation of alternative splicing of alpha-actinin transcript by Bruno-like proteins.
2002,
Pubmed
,
Xenbase
Takahashi,
The CUG-binding protein binds specifically to UG dinucleotide repeats in a yeast three-hybrid system.
2000,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Timchenko,
CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms.
1999,
Pubmed
Timchenko,
Novel proteins with binding specificity for DNA CTG repeats and RNA CUG repeats: implications for myotonic dystrophy.
1996,
Pubmed
Timchenko,
Molecular basis for impaired muscle differentiation in myotonic dystrophy.
2001,
Pubmed
Timchenko,
Overexpression of CUG triplet repeat-binding protein, CUGBP1, in mice inhibits myogenesis.
2004,
Pubmed
Timchenko,
RNA CUG repeats sequester CUGBP1 and alter protein levels and activity of CUGBP1.
2001,
Pubmed
Timchenko,
Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
1996,
Pubmed
Voeltz,
AUUUA sequences direct mRNA deadenylation uncoupled from decay during Xenopus early development.
1998,
Pubmed
,
Xenbase
